Introduction: Hypothesis: My hypothesis for this experiment‚ the study of the effects of different wavelengths on leaf pigments‚ if chlorophyll a absorbs blue-violet light‚ chlorophyll b absorbs blue light‚ carotene absorbs blue-green light‚ and xanthophyll absorbs blue light‚ then wavelengths of greatest absorption will be 400‚ 450‚ 500‚ and 450 respectively. Independent Variable: Wavelengths Dependent Variable: optimum wavelengths of light for absorption Standard Variable: method of
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Tittle : Absorption Spectra and the Beer-Lambert Law. ɛ Purpose : Understanding of Beer –Lambert Law Introduction : When light passes through the colour solution ‚ the molecules of the solution absorb the quantity of light at a particular wavelength . The amount of light which is absorbed by solution depend on two things. 1. The length of the pathway of light 2.Concentration of colour solution. These are all explained by the Beer-Lambert Law
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Extraction and Quantitative Determination of B-Carotene Introduction The study of B-Carotene is important because it is good for vision‚ immunity‚ and overall health. The purpose of this experiment is to extract and find quantitative amount of Beta-carotene from a carrot sample. The raw product(carrots) was extracted using simple distillation. The purity of the product will be characterized using percent yield‚ thin layer chromatography‚ Beer’s Law‚ and UV-vis. Beer’s law is used because in this
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Methods We began this experiment by turning the Spectrophotometer to 605 nm absorbance‚ and setting up each of the tubes as stated in Table 7-2*‚ ignoring the DPIP until the rest of the sample was set up. Once all of the samples were set up accordingly‚ we blanked the spectrophotometer‚ and added the DPIP to the sample right before‚ inverting the sample and taking a reading at the Zero time mark‚ and placing the sample in the designated light apparatus‚ Sample 1 was wrapped in aluminum foil to
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1. The standard curve is a graph that shows a relationship between concentration and absorbance of a solution. A standard curve was experimentally created in this experiment using 10mL solutions of phenol red with concentrations 10µM‚ 7.5 µM‚ 5.0 µM and 2.5 µM then the absorbance of each sample was measured using a spectrophotometer. This generated curve with resulting average absorbances of 1.273nm‚ 1.0275nm‚ 0.585nm‚ 0.285nm and 0.124nm provided a means to determine the phenol red concentrations
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The purpose of this lab was for students to familiarize and perfect the simple distillation method to produce 4-Methylcyclohexene and to gain experience using an IR spectroscopy to characterize the product of the reaction in favor of equilibrium. B. Results and Discussion: C. Experimental: 24 mL of 4-methylcyclohexanol‚ 5mL of phosphoric acid‚ and 1mL of concentrated sulfuric acid was added to a 250 mL round bottom flask and mixed thoroughly with a magnetic stirrer. A simple distillation
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Absorption Spectroscopy Author: Lab Partners: Instructor: Chem 152 Date Work Performed: Date Work Submitted: Abstract A Beer’s Law analysis was used to determine the molar absorptivity of a Red-40 food dye. Using a spectrometer‚ the best wavelength to use for the analysis of this dye was determined to be 501nm. A series of dilutions were performed and the concentrations were calculated to find ‘E‚’ the molar absorptivity‚ which was determined to be 18035 M-1 cm-1. Introduction
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Abstract This report investigates the absorbance of methylene blue and carmine red using a spectrophotometer to determine the absorption spectra of both solutions. The concentration of the unknown solution of methylene blue was found to be 1.07 x 10-5 M by using the molar extinction coefficient‚ with absorption of 0.547. It was also found that the results concluded confirmed beer’s law with an R2 value of 0.9989. Introduction Spectrophotometry is the quantitative measurement of the absorbance
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Introduction: Experiments 1 and 2 main aims are as follows: • To able to use the Beer-Lambert law to explain the relationship linking concentration and absorbance of the coloured solutions methyl orange and bromophenol blue. • To be able to rightly operate a spectrophotometer to assess the absorbance at various wavelengths • To be able to identify the main component parts of a spectrophotometer • To be able to draw a ‘Standard Curve’ from the data collected
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In the year 1803‚ Thomas Young first introduced the idea of trichromatic color representation. Young proposed that we have three “particles” in the eye that are each sensitive to a specific range of visible light that allows us to see color. Years later‚ Hermann von Helmholtz continued Young’s theory. Helmholtz suggested that the three different “particles” were categorized by their response the different wavelengths of light that hit the retina. Today‚ we know that the “particles” that Young and
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