Lab 4: Photosynthesis and Chromatography of Spinach Leaves Discussion: The chromatography of the spinach leaves was taken to establish what pigments were present in the chloroplasts of the leaves. The solvent carried and separated the different pigments up the cellulose paper. The solvent moved up the paper because of capillary action because of adhesion of the solvent molecules and the paper. The pigments dissolved in the solvent also were carried up the paper and were separated because
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experiment consisted of three smaller experiments as a result of the different properties chlorophylls possess and the need to test them individually to receive the most accurate results. Preparation for the lab began with selecting a medium to large spinach leaf and deveining it. Once the spinach was prepared‚ it was ground in 5 mL of 100% acetone‚ creating a green acetone extract. The extract was filtered through a funnel with a kimwipe and into a test tube. The volume of the filtered extract was
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Collecting Photosynthetic Rates in Spinach (Spinacia oleracea) Introduction: The purpose of this study was to see which light color would cause the spinach sample to photosynthesize the most within the given time. In order to understand photosynthesis‚ we need to know that chloroplasts absorb light energy from the sun‚ the plant then takes that energy along with water and carbon dioxide and changes it to sugar and oxygen. This happens in order for the plants to grow. But‚ light energy comes in a
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Title of Experiment Extraction of Spinach Date that the Experiment was Performed This experiment was performed on Wednesday‚ September 17th‚ 2014 at 2:45 pm in the St Ignatius Science Center Laboratory 323. Partners Names Taylor Jackson and Matt D’Angelo. Taylor‚ Matt‚ and I shared the same data. Purpose/Goals/Objectives The purpose of this experiment was for each student to use column chromatography to separate plant pigments from spinach leaves. Some goals and objectives were to
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experiment‚ trials were conducted to test hypothesis of whether all 40 circular spinach leaves would arise or stay at ground level in differential water temperature’s. Using a fluorescent light source gave us another perspective to keep in mind for it could be an advantage or loss to new current predications. Later in discussion‚ relationship to this experience among two other will
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Community Outreach & Education Program CHROMATOGRAPHY (Adapted from: Forensic Science Activities. University of Colorado Boulder Hughes Initiative. .) DESCRIPTION: Students will use paper chromatography to separate ink molecules and identify the pen used on an unknown sample of handwriting. Students will graph and analyze data they collect using paper chromatography. PURPOSE/GOAL: Students will be able to: • Gain understanding of the purpose of chromatography. • Measure and graph pigment separation
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In this experiment spinach‚ DPIP‚ phosphate solutions were placed under various filters and the percent orange light transmittance through the solution was measured to determine the rate of photosynthesis. This experiment was set up to discover how different wavelengths of light affect the rate of photosynthesis in a spinach‚ DPIP‚ phosphate solution. I proposed that the spinach‚ DPIP‚ phosphate solution under no filter‚ and therefore exposed to all light will have a highest percent orange light
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Use this document to report your findings from the LeafLab Exploration Experiment. The lab report consists of three sections: Data‚ Exploration‚ and Lab Summary. Data: copy any data‚ graphs‚ charts‚ or notes that you have saved in your LeafLab online notebook into this section. Exploration: Answer the questions. The questions in the Exploration section are the same questions in your LeafLab instructions. Lab Summary: Write a 100- to 200-word summary. Data To copy your data from your online
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In this lab‚ we extracted spinach pigments‚ and analyzed what colors of light these pigments absorb. By using TLC plate‚ hexane and acetone‚ I separated the pigments of spinach‚ and discovered that the main pigments were green and yellow. This works because with different polarities‚ pigments move at different rates. Hexane and acetone were also used to separate chlorophyll and carotene from spinach. Since they are polar‚ they can separate organic and inorganic things. From the experiment‚ I know
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Chromatography serves mainly as a tool for the examination and separation of mixtures of chemical substances. Chromatography is using a flow of solvent or gas to cause the components of a mixture to migrate differently from a narrow starting point in a specific medium‚ in the case of this experiment‚ filter paper. It is used for the purification and isolation of various substances. There are two phases in chromatography: 1. Stationary Phase – a solid that does not move. In this experiment was the
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