Gas Chromatography Purpose: The purpose of the gas chromatography lab is to find out how different substances interact with the surface of a solid. Chromatography is a separation technique that depends on the relative distribution of the components of a mixture between a mobile phase and a solid stationary phase. Chromatography measures the tendency of a substance to interact with the surface of a solid or to remain in a mobile phase. When doing a chromatography lab the mobile phase has to
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Materials and methods Preparation of saponins from sea cucumber: Saponins were isolated from the sea cucumber‚ Holothuria thomasi (purchased from Hurghada‚ Red sea‚ Egypt and identified by Zoology department‚ Faculty of science‚ Tanta‚ Egypt)‚ according to the method described by Hu et al.‚ (2010) with some modification. Air-dried body walls (750 g) of sea cucumber were grinded into powder and extracted with eight liter with refluxing ethanol. The combined extracts were evaporated with rotary evaporator
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OBJECTIVE The purpose of this experiment is to analyze mixtures of compounds prior to‚ during and after a separation scheme. This experiment also allows monitoring reactions of organic molecules‚ and determines the identity of a mixture of compounds. STRUCTURES AND PHYSICAL PROPERTIES OF REACTANTS [1] SOLVENTS | a. Hexane1Molecular Molarity: 86.18 g/molBoiling Point: 69 ºCMelting Point: -95ºCDensity: 0.659 g/mL at 25ºCWater Solubility: Insoluble in waterColor/Texture: Colorless/LiquidHazardous
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5.05 Mixtures and solutions Lab report Ink Chromatography Chromatography is also used to compare and describe chemical substances. The chromatographic sequence of sorbed substances is related to their atomic and molecular structures. A change in a chemical substance produced by a chemical or biological reaction often alters the solubility and migration rate. With this knowledge‚ alterations or changes can be detected in the substance. Chromatography serves mainly as a tool for the examination and
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Chromatographic Separation of Sodium Fluorescein and Methylene Blue In this two-part lab the students will: • Learn the techniques associated with thin layer and column chromatography. • Determine the effects of the stationary phase in separating a mixture by chromatography. • Determine the effects of the mobile phase in separating a mixture by chromatography. • Conduct a separation using column chromatography. In the first week‚ the students will determine the best solvent system for separating
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Hormone Control of Leaf Abscission Lab Report February 24‚ 2011 By Patrick Mills Introduction- Hormones are a chemical are transported within an organism and are highly specific chemical signals between cells. The hormones we used were lanolin‚ auxins(IAA)‚ cytokinins(CYT)‚ ethylene(ETH)‚ and gibberellins(GA) and each has a different purpose. Auxins will control cell elongation‚ cytokinins promote the act of cell division‚ ethylene causes the ripening of fruit‚ and gibberellins will elongate
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How Chromatography Helps The Human Race Chromatography is the separation of a mixture by passing it in solution or suspension or as a vapor (as in gas chromatography) through a medium in which the components move at different rates. “Chromatography is done by making of a mixture move past the solids‚ or across the surface of a solid‚ like paper. The mixture is poured onto a solid surface. As the different components of the liquid run down the solid‚ some of them move more slowly than other. A component
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Introduction Chromatography is a technique used to separate a mixture into its individual components. In this experiment‚ chromatography is done by using a thin layer chromatography (TLC) plate and filter paper. The word ‘chromatography’ itself means the separation of colours. However‚ nowadays‚ both coloured and colourless compound are used in chromatography by various methods. In chromatography‚ the separation process of mixture is mainly based upon the polarity and molecular weight of sample
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³Malunggay andSpinach Powder´ ACKNOWLEDGMENTS The study aimed to introduce the combination of Malunggay and Spinach powder inorder to make a natural‚ nutritious and practical supplement for all especially to thosehealth-conscious individuals.Nevertheless‚ this study wouldnt be possible without the everlasting love‚ care andassistance from the researchers family‚ who supported her in the procurement of the muchneeded logistics and references‚ cultivated and propagated the Malunggay and Spinachplants
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Column Chromatography of Plant Pigments Paul Ibarbia‚ Gene Paolo Jasmin‚ Gianpaolo Jimenez and Lorenzo Labicane* Department of Biology‚ University of Santo Tomas‚ Manila‚ Philippines Abstract Column chromatography of plant pigments is the separation of plant pigments extracted from Capsicum frutescens (siling labuyo). No slurry is prepared of the eluent; eluent with the stationary phase is set and then carefully poured into the column. The eluent are the solvents which are responsible for the
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