Theoretically‚ the spinach extract at 18oC should have a faster rate of reaction than the 5oC extract because higher temperature allows substrates to move more rapidly hence increases the chance of collision with the enzymes. Moreover‚ the experiment was low in accuracy as
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Title Page Chelsea Maksin Organic Chemistry Laboratory 1 Column and Thin Layer Chromatography: The Separation of Spinach Pigments Russ Hoburg 02/15/2012 Abstract The main goal in doing the column and thin layer chromatography was to separate spinach extract into its components based on polarity and then to analyze the components. The separation of the spinach extract was done using the column chromatography with the wet/slurry packing method. Alumina was inserted into the column to act as
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of Chlorophyll from Fresh Spinach and Investigation of the Photochemistry of Chlorophyll Chlorophyll a Chlorophyll b E5 - Extraction of Chlorophyll from Fresh Spinach E6 - Investigation of the Photochemistry of Chlorophyll The aim of this experiment is to investigate the photochemistry of chlorophyll. This experiment will be performed in two lab periods. In the first lab period you will extract chlorophyll‚ the green pigment in leaves‚ from spinach. In the second lab period
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Effects of Photosynthesis of Spinach Leaves in Different Light Conditions Introduction Photosynthesis is the process of converting solar energy‚ carbon dioxide (CO2)‚ and water (H2O) into carbohydrates (CH2O) and oxygen (O2). Sometimes the end product of photosynthesis is glucose (C6H12O6). Photosynthesis takes place in the chloroplasts of plant cells. Here membranous thylakoids are stacked in grana surrounded by the stroma. During the light reactions‚ pigments within the thylakoid membranes
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Column and Thin Layer Chromatography: The Separation of Spinach Pigment Abstract Spinach extract was separated into fractions containing compounds of similar polarity by column chromatography. Based on solid-liquid phase partitioning‚ this separation technique exploited the different polarity of the compounds in the spinach extract. Three fractions with different colors were obtained. The extract and its fractions were analyzed using thin layer chromatography (TLC). The TLC results showed that
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The central message of this advertisement is if you want to be strong like Popeye‚ eat their spinach. I can recall waking up Saturday morning watching the cartoon Popeye as a child. Popeye was always trying to impress Olive Oil. Popeye wasn’t the only one with eyes for Olive Oil‚ his nemesis‚ Pluto also had a very hostile interest towards Olive Oil as well. The cartoon would start out with Popeye planning something nice for Olive Oil‚ the day would be going perfectly. Pluto would notice Olive
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Abstract This study was undertaken to determine the relationship of different wavelengths of light and the rate of photosynthesis in spinach leafs. The rate of photosynthesis was measured every five min under light colors of white‚ green‚ red‚ blue and yellow under a light intensity of 2000 lux. The rate of photosynthesis was measured by the spinach disk method in which we replaced the air from the disks with sodium bicarbonate using a vacuum. Under photosynthesis‚ oxygen‚ a product of photosynthesis
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Experiment 3: Isolation of Chlorophyll from Spinach and Chromatography Performed February 14th and 16th By Kyle Herzog Organic Chemistry 344 Section 807 Spring 2012 Objective: The purpose of the experiment is to extract pigments from spinach leaves and separate them by column and thin layer chromatography‚ determining Rf values for the pigments. Equations: Rf= Distance travelled by a given compound/Distance travelled by a solvent solution 1. Crude- 4.25/5.5= 0.773 2. Carotene-
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A Spectrophotometric Analysis of the Absorption of Green Light Versus Red Light Absorption in Spinach Leaves The goal of the experiment was to determine if green light had less ability to absorb than red light in spinach leaves. This was done by separating the photosynthetic pigments (chlorophyll a‚ chlorophyll b‚ carotene and xanthophylls) from one another using paper chromatography. The separated pigments were then analyzed for their absorption spectrum using a spectrographometer. When the
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this lab experiment‚ we observed photosynthesis in spinach leave and investigated the effect of sodium bicarbonate on photosynthesis. The experiment was to determine how many disks of spinach leaves float at the end of every ten minutes. Our main focused and question was how using different amount of concentration of sodium bicarbonate would affect the process of photosynthesis producing carbon dioxide. Our hypothesis was we will see more spinach disk floating because they are producing carbon dioxide
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