the right numbers for our calculations. Mixture is defined as a substance made by mixing other substances together‚ in this case we will be dealing with salt and sand of course to undergo the process of separation. Distillation‚ Filtration‚ and Chromatography are known alternatives to separate the
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Theoretically‚ the spinach extract at 18oC should have a faster rate of reaction than the 5oC extract because higher temperature allows substrates to move more rapidly hence increases the chance of collision with the enzymes. Moreover‚ the experiment was low in accuracy as
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Post Lab #4- Column Chromatography Organic Chem 3418-2 March 3‚ 2011 Theoretical Background- The fluorene and fluorenone mixture was separated by first dissolving the mixture in heptane. Since “like dissolves like”‚ fluorene dissolves with the non-polar heptane and the polar fluorenone dissolves in the polar ethyl acetate solvent. This phenomenon was illustrated in class before the experiment‚ when it was pointed out why water will not dissolve fluorene‚ fluorenone‚ or transstilbene
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RADICAL HALOGENATION AND GAS CHROMATOGRAPHY Abstract In radical halogenations lab 1-chlorobutane and 5% sodium hypochlorite solution was mixed in a vial and put through tests to give a product that can then be analyzed using gas chromatography. This experiment was performed to show how a radical hydrogenation reaction works with alkanes. Four isomers were attained and then relative reactivity rate was calculated. 1‚1-dichlorobutane had 2.5% per Hydrogen; 1‚2-dichlorobutane had 10%; 1‚3-dichlorobutane
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Heat on the pH of the Vegetable and Their Pigment PURPOSE The purpose of this experiment is to see how pH levels of certain vegetables change while being cooked in four different mediums (frying‚ boiling‚ steaming‚ roasting)‚ and how the varying pH levels change the coloring of the vegetables. We will analyze how the different method of cooking/heating changes the levels of pH. BACKGROUND INFORMATION This experiment is based around the different pigments in vegetables‚ of which we researched and
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Abstract This study was undertaken to determine the relationship of different wavelengths of light and the rate of photosynthesis in spinach leafs. The rate of photosynthesis was measured every five min under light colors of white‚ green‚ red‚ blue and yellow under a light intensity of 2000 lux. The rate of photosynthesis was measured by the spinach disk method in which we replaced the air from the disks with sodium bicarbonate using a vacuum. Under photosynthesis‚ oxygen‚ a product of photosynthesis
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Figure 1: Affinity chromatography of fumarase with the Ni2+-NTA-agarose column. Extract (9.9 mL) containing yeast (3.76g) in extraction buffer containing 0.1% Igapel CA-630 and protease inhibitors were pumped through Ni2+-NTA-agarose column. Fractions were collected by 1.5 mL portions by use of wash buffer (20.0 mL)‚ imidazole elution buffer (26.3 mL)‚ and wash buffer (10.0 mL)‚ again. Absorption readings were taken for all fractions with a Cary50 set at 280nm. The fumarase activity was determined
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Abstract Pigments extracted from different greens have different polarities and may be different colors. Mixed pigments can be separated using chromatography paper. Chromatography paper is able to separate mixed pigments due to their polarity and solubility. Pigments of chlorophyll a‚ chlorophyll b and beta carotene will be separated on chromatography paper because each has its own polarity and solubility‚ which results in different distance traveled up the paper. Beta carotene is non-polar so it
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The main objective of the present work is to characterize some pigments from the tomb of Nakht-Djehuty (TT189)‚ time of Ramesses II (c.1279–1212 BC)‚ El-Assasif district‚ El-Qurna necropolis‚ Luxor (ancient Thebes)‚ Upper Egypt. The characterization of the examined wall paintings was carried out by means of optical microscopy (OM)‚ scanning electron microscopy (SEM) equipped with an energy dispersive X-ray detector (EDS)‚ X-ray diffraction analysis (XRD)‚ Fourier transform infrared spectroscopy (FT–IR)
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Thin Layer Chromatography of Unknown Samples and Paprika By: Uyen Huynh Date: June 18‚ 2014 Lab Partners: Philip Murray Professor and section: M. Pandey‚ CHM2210L.601 Data: Analysis of Drug Store Items Knowns Rf Values Aspirin .91 cm Acetaminophen .85 cm Ascorbic Acid .86 cm Unknown .97 cm Identity of unknown mixture Acetaminophen Analysis of Paprika Pigments Spot Number Rf Color 1 1 Red-orange 2 1 Red-orange Calculations
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