REPORT NAME :princess cucumber TOPIC : 3.8 & 8.2 : PHOTOSYNTHETIC PIGMENTS RESEARCH QUESTION INTRODUCTION The photosynthetic pigments are responsible for absorbing and trapping light energy the major pigments of photosynthesis are the chlorophylls. The two chlorophylls found in green plants are chlorophyll a and chlorophyll b. other pigments include carotenoids and phycobilins‚ sometimes referred to as accessory pigments. Carotenoids occur in all photosynthetic organisms‚ while phycobilins
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Experiment 3: Isolation of Chlorophyll from Spinach and Chromatography Performed February 14th and 16th By Kyle Herzog Organic Chemistry 344 Section 807 Spring 2012 Objective: The purpose of the experiment is to extract pigments from spinach leaves and separate them by column and thin layer chromatography‚ determining Rf values for the pigments. Equations: Rf= Distance travelled by a given compound/Distance travelled by a solvent solution 1. Crude- 4.25/5.5= 0.773 2. Carotene-
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Chemistry Laboratory I Experiment #6 - Column and Thin-Layer Chromatography (TLC) of a Spinach Extract Pre-Lab Assignment (1) List 5 ways that TLC can be used in an organic chemistry lab experiment. To identify an unknown‚ to monitor the course of a reaction and assess the purity of its product‚ to determine the best solvent for a column chromatography separation‚ to determine the somposition of each fraction from a column chromatography separation‚ and to determine whether a substance purified
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Chromatography – Analyzing Analgesics by TLC and Isolation of β-Carotene by Column Chromatography Introduction/Background: Flavonoids are an important group of additives that can be defined as pure substances either natural‚ extracted from raw materials or synthetic. Chromatography is the separation of two or more compounds or ions caused by their molecular interactions with two phases – one moving and one stationary (Weldegirma 2012). Three types of chromatography are used
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INTRODUCTION Plant pigments consist of four main chemicals. These are Chlorophylls‚ The carotenoids‚ Anthocyanin and Betacaine. The aims of this experiment is to separate the plant pigment into different colours using solvents and column chromatography. The hypothesis of this experiment is that this plant pigment from spinach will contain the chlorophyll pigment or may contain the others too. Chlorophyll: Chlorophyll a is the main component of the plant pigments. This is used for the most
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COLUMN AND THIN LAYER CHROMATOGRAPHY Maria Janine B. Abarientos‚ Kuia B. Allarde‚ Aliana Keshia P. Andino Mary Viadelle E. Andrada and Nina Marian Robelea G. Ang Group 1 2C Pharmacy Organic Chemistry Laboratory ABSTRACT In this experiment‚ the techniques column and thin layer chromatography was used to separate and determine the purity of the colored components of siling labuyo and malunggay leaves. The results obtained relied on differential solubilities and adsorptivities of the components to
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Paper chromatography is one of the easiest methods of chromatography. It is a method of planar chromatography (stationary phase is in form of a plane). Paper chromatography follows the basic principle of chromatography‚ which states that substances or components are distributed in between the stationary phase and the mobile phase. It is an analytical technique‚ where only a small amount of a sample is used for separating and identifying its components. Like any other method of chromatography‚ paper
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Mr. Sousa Organic Chemistry ACL 8 January 2015 Chromatography Chromatography is a physical method of separating substances based on their properties‚ by distributing their components between a mobile and stationary phase. Chromatography is useful for observing mixtures and solvents‚ since it can be used to determine the relative bond strength of various compounds‚ a substances phase‚ and it can also the identity of unknown substances. Chromatography allows for the separation of chemical mixtures
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OBJECTIVES: The objective of this experiment was to extract plant leaf pigments and determining them by using the Rf values obtained from the paper chromatography technique. The hypothesis of the experiment was that all of the five listed pigments would be present in the extracted plant leaf according to the Rf values. PROCEDURE/APPARATUS: The equipments used were a 18 x 150 mm test tube with stopper‚ graduated cylinders‚ Erlenmeyer flask‚ mortar and pestle‚ metric ruler‚ tall jar‚ acetone‚ tiny
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three smaller experiments as a result of the different properties chlorophylls possess and the need to test them individually to receive the most accurate results. Preparation for the lab began with selecting a medium to large spinach leaf and deveining it. Once the spinach was prepared‚ it was ground in 5 mL of 100% acetone‚ creating a green acetone extract. The extract was filtered through a funnel with a kimwipe and into a test tube. The volume of the filtered extract was estimated‚ and one half
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