Chlorophyll and Carotenoid Pigments from Spinach Purpose The purpose of this experiment was to isolate ß-carotene‚ chlorophyll-A‚ and chlorophyll-B from spinach using column chromatography. Spinach was dehydrated using ethanol‚ and the pigments were extracted with dichloromethane. The spinach extracts were dried using CaCl2. Then‚ the solid pigments were run through a column using a non-polar solvent‚ hexane. The polar absorbent material in the column separated the different pigments by allowing the least
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crystal constituents of the compound. Thin layer chromatography can be used as a physical method to segregate compounds from natural sources. E.g. Spinach leaves are visibly green‚ but consist of a variety of components that have more colour than others. This experimental procedure uses compounds from spinach leaves that are exposed to chromatography‚ TLC plate to indicate the different pigments within the spinach extract. ‘Column chromatography in chemistry is a method used to purify individual
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BIOLOGY 1010L – BOTANY Laboratory Module 8. Plant Physiology (2): Plant Pigment Paper Chromatography All organisms need energy for their metabolic processes. They also need “food” to produce that energy. Plants are autotrophs (self-feeders). Plants produce their food through a process called Photosynthesis. The food that they produce is the sugar glucose. Animals and other organisms are heterotrophs (other- feeders). They must consume other organisms (plants) in order to eventually get their
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LABORATORY REPORT FOR BIO411 Experiment 5 : Cellular Respiration Experiment 6 : Photosynthesis Title: Cellular Respiration Objective: To observe and determine cellular respiration in yeast/onion cells. * Measure respiration rate using different substrates. * Measure respiration rate at different temperature. Introduction: In this laboratory experiment‚ we are given 3 task. The first one is respiration in yeast. Second is respiratory indicator and the third one is observing
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spectrum) by James Clerk Maxwell; this included: cosmic rays‚ visible light‚ x-rays‚ and radio waves. For plants to use sunlight‚ they have to absorb it. So pigments absorb light energy. Chlorophyll is a pigment in leaves that reflect green light waves. This is the most important pigment in photosynthesis and there are also accessory pigments used in plants. Also in this lab there is the internal and external anatomy of a typical leaf. Materials and methods: The first procedure dealt with the
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Introduction The purpose of this lab is to determine which pigments in a plant support or effect photosynthesis‚ based on starch production‚ which wavelengths of light are involved in photosynthesis‚ and identify plant pigments found in a plant leaf by means of paper chromatography. Life on Earth is dependent entirely on the energy from the Sun‚ not only to keep the planet at a suitable temperature but also to provide the energy required to sustain life. The energy of the Sun‚ in the form of
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ABSTRACT Rising energy prices and environmental problems led to the increase in alcohol demands in many aspects‚ like in making fuels. Many people are doing researches about making their own product so as to lessen their everyday expenses. Right now‚ Filipino scientists are in think of the search for alternative fuels‚ with the experts from the University of the Philippines in both Diliman and Los Banos campuses searching all over the archipelago for energy sources from the forests to the bottom
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Plant Pigments and Photosynthesis Problem: We are going to separate and identify pigments and other molecules from cell extracts through a process called chromatography. We will also test the theory that light and chloroplasts are required for light reactions to occur. Background: Pigments are chemical compounds which reflect only certain wavelengths of visible light. This is what makes them seem colorful. Many things contain pigments including flowers‚ corals‚ and even skin. More important than
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Column Chromatography ________________________________________________ You have already performed two chromatography experiments: gas chromatography and thin layer chromatography. All chromatography experiments involve passing a mixture of analytes through a system that includes a mobile phase and a stationary phase. The partitioning of the analytes between these two phases determines the rate at which they pass through the system‚ and (in theory) allows them to be separated from one another. Column
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the silica. More eluent was added and drained until it was level with the top of the sand. Extraction of pigments 3-4 baby spinach leaves were gathered and placed into a mortar with a small amount of sand. 8mL of methanol was measured out and added to the mortar. A stemmed funnel lined with a filter was clamped and placed into a 125mL separatory funnel (with stopcock closed). The spinach was crushed with a pestle until it turned a dark green
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