Introduction In order to understand what HPLC‚ that is‚ High Performance Chromatography‚ it is necessary to understand the meaning of the term chromatography itself. Chromatography Chromatography is defined as a procedure by which inert materials and drug principles encountered in pharmaceuticals preparations are separated by fractional extraction‚ adsorption‚ or ion exchange on a porous solid. Chromatography is particularly useful as a means of separating and purifying complex and closely
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Post Lab Report Experiment 3 - Chromatography – Analyzing Analgesics by TLC and Isolation of β–Carotene by Column Chromatography Chemicals 1. Acetaminophen (C8H9NO2) 2. Aspirin (C9H8O4) 3. Caffeine (C8H10N4O2) 4. Ibuprofen (C13hH18O2) Introduction In this experiment‚ several analgesics were analyzed by Thin Layer Chromatography (TLC) and the composition of an unknown tablet was identified. We define chromatography as the separation of two or more compounds or ions by
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Candy Chromatography By: Avani Reddy 7th Grade Introduction Ever wondered why candies are different colors? Ever wondered why candies are different colors? Many candies contain colored dyes. Bags of M&Ms or Skittles contain candies of various colors. The labels tell us the names of the dyes used in the candies. But which dyes are used in which candies? We can answer this by dissolving the dyes out of the candies and separating them using a method called chromatography. Research
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heart complications‚ kidney problems‚ asthma and digestive disorders. Each ounce of Malunggay contains seven times the Vitamin C found in oranges‚ four times the Vitamin A of carrots‚ three times the iron of spinach‚ four times as much calcium as milk and three times the potassium of bananas. Spinach is well-known in stabilizing the blood sugar among diabetics‚ it serves as an anti-oxidant‚ an anti-cancer agent‚ it prevents from atherosclerosis or thickening and hardening of arteries and it aids in the
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Analytical techniques Introduction * The analysis technique chosen is gas chromatography‚ I’m going to talk about it and I am elected chromatography studies to identify and quantify known materials do. * The principles of gas chromatography * The functions of gas chromatography * Theories of instruments and techniques etc. PRINCIPLES Gas chromatography (GC) was first demonstrated for analysis of liquid in the classic paper by james and martin (1952). It was quickly recognised
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this lab experiment‚ we observed photosynthesis in spinach leave and investigated the effect of sodium bicarbonate on photosynthesis. The experiment was to determine how many disks of spinach leaves float at the end of every ten minutes. Our main focused and question was how using different amount of concentration of sodium bicarbonate would affect the process of photosynthesis producing carbon dioxide. Our hypothesis was we will see more spinach disk floating because they are producing carbon dioxide
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ACKNOWLEDGMENTS The researchers would like to extend their heartfelt thanks to the following people who helped to make this investigatory project successful. The researcher would never forget to acknowledge their Science & Technology III teacher‚ Mrs. Letecia O. Zaldarriaga for extending her invaluable time‚ patience in explaining‚ giving important ideas and correcting their write ups. Heartfelt gratitude where also given to the parents of the researchers‚ who uplifted the researcher emotionally
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January 27‚ 2014 Introduction This laboratory has its first objective to familiarize with the thin layer chromatography (TLC) technique. Second‚ each student has to identify the unknown components by comparing it to one of the tree dissolved liquid analgesics: Acetaminophen‚ Aspirin and Caffeine. Finally‚ calculating the Rf value by measuring the mobile and stationary phase. Chromatography is a technique used to separate mixtures into their compounds. It’s based on a mobile phase and a stationary
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Bangabandhu Sheikh Mujibur Rahman Science and Technology University‚ Gopalganj. Title : Assignment on HPLC (High-performance /High-Pressure Liquid Chromatography) Introduction: HPLC is an advanced form of liquid chromatography used in separating the complex mixture of molecules encountered in chemical and biological systems‚ in order to recognize better the role of individual molecules by using significantly higher pressure (50–350 bar). Compounds are separated by injecting a sample
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Proteins (ACTIVITY 8) Gallemit‚ Lacei Machelle• • January 17‚ 2013 ABSTRACT Chromatography is one of the most useful methods of separating minute amounts of complex mixtures into the pure substances contained therein. Chromatographic separations are effected by allowing the components in the mixture to be distributed in two phases‚ one being stationary‚ and the other‚ mobile. Partition chromatography involves two main distribution systems‚ one system is composed of a gas and a liquid and
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