Chromatography: How can we separate a mixture? Purpose The chromatography lab is to understand how molecules with similar molecular properties can be separated with paper chromatography. These differences will be interpreted to see the distinction of separate chemical substances. Pre Lab Questions 1. Explain capillary action as it pertains to water and paper. Capillary action makes water draw up the paper. As paper absorbs water mixes with the solutions in the paper. 2. What is the
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provided for. Good luck!!! CHROMATOGRAPHY (By: Mayflor Markusic) KALIKASAN: Everyday Science in Action Volume 4 No. 1; pages 22-24 a) What is chromatography? 1 2 3 4 ____________________________________________________________ ________________ b) Give examples of mixtures which can be separated through chromatography. 4 5 6 7 ________________________________________ ______________________________________ c) How is chromatography done? 4 5 6 7 ________
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Paper chromatography is an important separation technique that depends upon differences in how strongly the dyes are adsorbed onto the paper (stationary phase) and how soluble the dyes are in the developing solvent (mobile phase). In paper chromatography‚ a small amount of the mixture to be separated is placed close to the edge of a piece of paper. The edge of the paper is then immersed in a developing solution. As the developing solution ascends up the paper by capillary action‚ the. components
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The History of Paint Pigments Chem 111-404 April 24‚ 2013 Introduction: This report expresses five different pigments that were formed during chemical reactions. Three out of the total five reactions went through a metathesis‚ or double-replacment reaction. A metathesis reaction can be defined as “a chemical reaction in which an element or radical in one compound exchanges places with another element or radical in another compound.” (Webster). The other two pigments underwent a neutralization
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Lab 11- Plant pigment and Photosynthesis Zelda Salfati December 20th‚ 2012 BIOL 1012Y Partners Lab: Nicole Mintz‚ Bayla Kronman
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results did not support the hypothesis. Referring to table‚ one can see that blue caused both the spinach and radish plant to grow the most and that green light cause the plants to grow less. Spinach is a long-day plant therefore it was able to grow better under a constant light source. This would have aided in the plant growing faster than other forms of plant species. When experimenting with the spinach plant blue light worked exceedingly well in making the plant grow faster with there being a decent
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Search the web. Some interesting sites are listed below. Note that some of these sites go into much more depth than is reasonable for this course. http://en.wikipedia.org/wiki/Chromatography http://ull.chemistry.uakron.edu/analytical/Chromatography/ http://orgchem.colorado.edu/hndbksupport/TLC/TLC.html this is for TLC – similar to paper http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/C/Chromatography_paper.html http://jchemed.chem.wisc.edu/JCESoft/Programs/CPL/Sample/modules/paprchrom/paprchromdesc
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Liquid Chromatography Purpose: The purpose of this lab was to separate substances based on their polarity by using liquid chromatography. Data Table: Red Dye Blue Dye Run#1 Run#2 Run#3 Run#1 Run#2 Run#3 Start of Band(mL) 1.50 2.20 1.00 2.70 3.00 2.00 End of Band(mL) 2.70 3.00 2.00 6.40 5.50 6.00 Beaker Eluant Observations 1 H2O White powder 2 5%isopropyl Red powder 3 28%isopropyl Blue powder 4 70%isopropyl Oily residual Calculations: W = Vend – Vstart
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experiment‚ trials were conducted to test hypothesis of whether all 40 circular spinach leaves would arise or stay at ground level in differential water temperature’s. Using a fluorescent light source gave us another perspective to keep in mind for it could be an advantage or loss to new current predications. Later in discussion‚ relationship to this experience among two other will
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In this lab‚ we extracted spinach pigments‚ and analyzed what colors of light these pigments absorb. By using TLC plate‚ hexane and acetone‚ I separated the pigments of spinach‚ and discovered that the main pigments were green and yellow. This works because with different polarities‚ pigments move at different rates. Hexane and acetone were also used to separate chlorophyll and carotene from spinach. Since they are polar‚ they can separate organic and inorganic things. From the experiment‚ I know
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