First‚ the amount of total protein removed by the Affinity Chromatography is difficult to quantify because there appears to be an error in the analysis data for 65% cut precipitate. The protein from the previous purification step limits the amount of protein that can be recovered by the subsequent purification step. In this case‚ the total protein recovered by 65% cut was 159 mg‚ but Affinity Chromatography recovered 350 mg of protein. Purification steps cannot gain protein unless
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notes on the relationship between the structure‚ properties and functions of glycogen and cellulose. 10) Write notes on the 3 main types of column chromatography (not HPLC)‚ explaining their mode of action and principal uses. 11) Write notes on TWO of the following chromatographic techniques: Thin layer chromatography‚ gel (size exclusion) chromatography‚ ion exchange
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Extraction * 6 Precipitation and differential solubilization * 7 Ultracentrifugation * 8 Chromatographic methods * 8.1 Size exclusion chromatography * 8.2 Separation based on charge or hydrophobicity * 8.3 Ion exchange chromatography * 8.4 Affinity chromatography * 8.4.1 Metal binding * 8.4.2 Immunoaffinity chromatography * 8.4.3 Purification of a tagged protein * 8.5 HPLC * 9 Concentration of the purified protein * 9.1 Lyophilization
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mango 4 ounces of salmon 1 cup of raw almond butter ½ head of cabbage 2 lemons ¼ cup of Honey 2 tablespoons of fresh ginger ½ tablespoon of red chili 1 head of spinach Cilantro Basil ½ head of lettuce (large leaves only) Salt Preparation: To begin‚ remove the lettuce leaves from the head‚ and pluck the largest spinach leaves from the bunch. Place them in a bowl of chilled water to soak.
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LEE ET AL.: JOURNAL OF AOAC INTERNATIONAL VOL. 88‚ NO. 5‚ 2005 1269 DIETARY SUPPLEMENTS Determination of Total Monomeric Anthocyanin Pigment Content of Fruit Juices‚ Beverages‚ Natural Colorants‚ and Wines by the pH Differential Method: Collaborative Study JUNGMIN LEE U.S. Department of Agriculture‚ Agricultural Research Service‚ Pacific West Area (PWA)‚ Horticultural Crops Research Laboratory Worksite‚ 29603 University of Idaho Ln‚ Parma‚ ID 83660 ROBERT W. DURST and RONALD E. WROLSTAD Oregon
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IDENTIFICATION OF UNKNOWNS BY TLC AND MP IN COMBINATION Introduction: Thin layer chromatography (TLC) is one of the most valuable techniques in organic chemistry. This is a best method of separating and identifying mixtures of two or more compounds. The separation is accomplished by the distribution of the mixture between two phases: one that is stationary and one that is moving or mobile. Chromatography works on the principle that different compounds will have different solubilities and adsorption
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solutions: Lab report: outline Ink chromatography Chromatography is the separation of a mixture by passing it in solution or suspension or as a vapor (as in gas chromatography) through a medium in which the components move at different rates. It is used to separate substances. Examples of chromatography are finger-printing‚ medicines‚ and separating/ testing histamines. http://www.biotecharticles.com/Agriculture-Article/Applications-of-Chromatography-1299.html The purpose of this lab
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◆ Bring in a can of spinach; a can opener (electric‚ if possible); small disposable bowls; and disposable spoons. Ask one student to act as the recorder again. ◆Have students name the five senses as the recorder lists them as column headings on the board. Then‚ use the can opener to open the can of spinach. ◆ Encourage students to share words that describe how it sounds. Spoon the contents of the can into bowls. Give each student a bowl containing a small serving of spinach. Ask students to describe
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List of Chemistry Terms used in daily life α (alpha)‚ β (beta) α- (β-‚ γ-) ray spectrometer α-addition (alpha-addition) α-cleavage (alpha-cleavage) α-decay (alpha-decay) α-effect α-elimination α-expulsion in photochemistry α-oxo carbenes α-particle (alpha-particle) ’A’ value -factorquantity ab initio quantum mechanical methods abatement in atmospheric chemistry abeo- abiological abiotic abiotic transformation absolute activation analysis absolute activity‚ quantity absolute
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CHM130 Lab 9 Chromatography Name: Karlee Rose A. Data Table (12 points) Paper # Color Source Solvent Distance Component Moves Distance Solvent Moves Rf value 1 Yellow M&M Candies 0.1% Salt Solution 28.88mm 42mm 0.69 2 Yellow Reese’s Pieces 0.1% Salt Solution 16.95mm 32mm 0.53 3 Purple Grape Soda 0.1% Salt Solution 32.15mm 51mm 0.63 4 Purple Grape Koolaid 0.1% Salt Solution 12.12mm 31mm 0.39 5 Red Easter Egg Dye 0.1% Salt Solution 1.18mm 7mm 0.17 6 Red Dry Erase Marker
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