University of Texas at Tyler Lab 3C: Purification of L-Lactate Dehydrogenase By Affinity Chromatography on Cibacron-Blue Sepharose David Alexander 10-15-2014 Dr. Black Chem 4135.001 Abstract: Like the previous experiments‚ the ultimate goal of this lab was to purify the enzyme sample. However‚ this is the last lab for purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally
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Acid Base Titration Purpose: The purpose is to calculate the molarity of a NaOH solution by titrating the base with 5mL of standard HCl solution in each trial. By adding the base with unknown molarity to the acid with 0.10M the molarity of NaOH can be calculated. The base‚ NaOH‚ helps bring the pH of the acid‚ HCl‚ closer to seven‚ which neutralizes it. When using the buret the amount of NaOH used is able to be determined. Then by writing a balanced chemical equation and using the titration
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Roy Levin Bio 11 Lab Dr.Izquierdo Analysis of Macromolecules in Tissue Homogenates of Bos taurusMaterials and Methods The homogenates provided were made by homogenizing tissues in a sucrose phosphate buffer in a 1:20 ratio. The protein concentration in bovine cells was measured by diluting the homogenate with a 1:5 ratio; 50 microliters of homogenate and 200 microliters of water. Then 5 known protein concentration samples which were 0.4‚ 0.8‚ 1.2‚ 1.6‚ 2.0 mg/ml of bovine serum were used to
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Spot-fixing/Match-fixing The great damage done to the Cricket is by match-fixing allegations every now and then. Spot-fixing means that player or the whole team accepts money from the bookies to intentionally play badly. Players also have been taking money to give information about the pitch and the weather conditions. Generally players deny any kind of charges put on them‚ but in some cases players have admitted their involvement in either spot-fixing or illegal activities. For instance
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Practical Report Name: Loh Khin Keong Admin No.: 1237078 Group number: A Date: 11/5/12 Class: DMLS/1A01 Experiment 1A: Determination of Mechanical Properties of Selected Materials (CDIO) 1. Synopsis The objective of this CDIO experiment was to determine the mechanical property of polymeric materials which was the tensile properties .The testing standard to adopt for the tensile testing was ASTM D638. Four different types of polymers were placed into the tensile testing machine. The different specimens
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:___________ Chemistry Laboratory 101__ Date Submitted[1] :___________ Members[2]: Instructor’s Initials[3] :___________ 1. _____________________ 2. _____________________ 3. _____________________ 4. _____________________ Laboratory Report Sheet The Bunsen Burner Activity 1 Objectives:4 1. ________________________________________________________ 2. ________________________________________________________ 3. ________________________________________________________
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store requests more often than not. However‚ the question remains‚ is this input in the form of a survey or review? In this case it is in the form of a survey‚ and after carefully analyzing the responses given by Finale’s customers‚ I have discovered some useful information to guide management actions. Based on my analysis of Exhibit 1‚ I believe Finale’s guests seem to be more couple oriented. Also‚ 41% of the respondents indicated that they visit Finale less frequently‚ which seems to hint to a
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Abstract: This report presents the reason why the ocean does not freeze in the winter. Since the ocean consists of salt water‚ an experiment was done to test the effect of freezing on salt water compared to pure water. The experiment was conducted three times in order to obtain accurate results and eliminate errors. In addition to the salt and pure water experiment‚ a variation of this experiment was created to test other options. In the variation experiment the effect of freezing on sugar water
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the period of oscillations to the length of the pendulum.Sources of error for this procedure included precision in both length and time measurement tools‚ reaction time of the stopwatch holder‚ and the accuracy of the stopwatch with respect to the lab atomic clock. The final result of g takes into account the correction for the error introduced using the approximation. There are opportunities to correct for the effects of mass distribution‚ air buoyancy and damping‚ and string stretching[1]. Our results
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Organic Chemistry II Lab 9 Fermentation of a Carbohydrate: Ethanol from Sucrose * Introduction Ethanol is one of the oldest alcohols and also the least toxic one. Industrially‚ ethanol is made most economically by hydration of ethylene. However‚ ethanol that is intended for human consumption must‚ by law‚ be prepared by fermentation. By either method‚ ethanol‚ of course‚ has the same formula‚ structure‚ and properties. The fermentation takes place with the assistance of enzymes from yeast
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