TITLE AND AUTHOR Lab 7 Analysis of purified Concanavalin A via:Hemagglutination INTRODUCTION The purpose of this lab was to test the biological activity of ConA by performing a hemagglutination assay. If ConA is active then agglutination will occur due to ConA’s free receptors being able to bind to the glucose residues on the sheep’s red blood cells. If ConA is not active then no agglutination will occur. To test the hemagglutination reaction‚ two types of ConA solutions
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There are quite a few variations between normal and tumor cell division. Normal cell division can be broken into four phases: G1‚ S‚ G2‚ and M. During the G1 phase‚ RNAs are produced‚ proteins are synthesized and through the P53 gene (also known as the “Guardian of the Genome”)‚ cells are checked for damage and those that are found are forced to go through apoptosis where the cells are forced to “commit suicide” to prevent replication. Through the S phase‚ the DNA is duplicated and in the G2 phase
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treatment of a pH shock and neutralization‚ by depicting a constant percentage of immobile cells. Prior to the lab series‚ it was expected that the positive control would present a decreasing percentage of immobile cells‚ and the negative control would present an increasing percentage of immobile cells. The experimental groups‚ testing the hypothesis‚ was inferred to show a constant number of immobile cells‚ after the 60-minute light treatment. Colchicine is a substance that reduces inflammation
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Stage Number of Cells in Part 1 Number of Cells in Part 2 Interphase 11 9 Prophase 7 4 Metaphase 5 3 Anaphase 9 7 Telophase 4 3 Cytokinesis 2 2 Create a Graph that represents the time spent in each stage of the cell cycle. Stage Time Spent in the 1st stage Time spent in the 2nd stage Interphase 21% 25% Metaphase 13% 7% Anaphase 9% 16% Telophase 19% 18% Cytokinesis 20% 23% 18% 11% Hypothesis After researching‚ I predicted that I would see different numbers of cells (which
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BioLab3 Lab Report 7 Cellular Respiration Answer Key Student Name: I. The ATP Cycle Define the following terms: Autotroph Heterotroph Aerobic respiration Anaerobic respiration Complete the chart below: Letter Defining Term A B C D II. Anaerobic Respiration Define the following terms: Alcoholic fermentation EXERCISE 1 – Alcoholic fermentation At intervals of 20‚ 40‚ and 60 minutes‚ the tubes are removed. Record the
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3.01 Cell Cycle Lab Report Safety Notes: * Always handle microscopes and glass slides carefully. * Wash your hands after handling the prepared specimens. Materials: * Compound light microscope * Glass microscope slide with prepared onion root tip specimen Purpose: * understand and identify the stages of the cell cycle and mitosis. * apply an analytical technique to estimate the relative length of each stage of the cell cycle. Hypothesis Procedure: I predict that
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The Effect of Temperature on Beet Cell Membranes and Concentration of Betacyanin Released Introduction Membranes are an important feature of plant cells and they act as a barrier that separates the interior of the cell from the external environment (Campbell et al.‚ 2008). Each membrane layer is composed of a phospholipid layer‚ which is semi-permeable and possesses the ability to control the movement of diffusion. Within the centre of a cell‚ the vacuole that is responsible for storage and
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Cell Surface Glycoprotein Receptor Analysis Using Concanavalin A Purpose: During this experiment we compared the hemagglutination reaction of control Con A solution at 2 mg/ml in Con A buffer with the hemagglutination reaction of your own purified Con A sample that you diluted previously at 2 mg/ml in Con A buffer. The purpose of this lab was to determine the strength of the reaction by performing serial dilutions on both the Con A sample and the control Con A sample‚ and determine through observations
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Differentiating Between Prokaryotic and Eukaryotic Cells‚ Distinguish Among Plant‚ Animal‚ and Protist Cells‚ and Identifying the Organelles that are Evident in Them Introduction There are two different types of cells‚ prokaryotes and eukaryotes. Prokaryotes‚ such as bacteria‚ lack a nuclear membrane and other membrane bound organelles. Their genetic material consists of a single molecule of singular DNA. Eukaryotes‚ such as plant and animal cells‚ have a nuclear membrane and other membrane bound
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the female parent. 2. Highlight the letter of each way to describe a diploid cell. a. 2N b. Contains two sets of homologous chromosomes c. Contains a single set of homologous chromosomes d. A gamete 3. Highlight the letter of the number of chromosomes in a haploid cell where 2n=8 . a. 8 b. 4 c. 2 d. 0 4. Is the following sentence true or false? The diploid cell that enters meiosis becomes 4 haploid cells at the end of meiosis.false 5. How does a tetrad form in prophase I of meiosis
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