different colonies were observed‚ the first colony was yellow in color and larger in size and the white colored colony was slightly smaller in size. As instructed‚ each colony was prepared for gram staining‚ one slide for the large yellow colony and one for the smaller white colony. After properly gram staining the slides as directed in chapter six of the lab manual‚ the smears were examined
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Title – Differentiating Organisms using the Gram Stain Introduction The experiment conducted was based upon the known attributes of two different groups of bacteria‚ those that are gram positive‚ and those that are gram negative. Using a specific staining procedure‚ it is possible to differentiate the two types under a microscope The gram stain method of differentiation is possible because of differences in the cell membrane between the two categories of bacteria. Gram positive cells have an extra
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multiple types of testing in order to determine the identity of the unknown organism. The tests performed to identify the unknown organism included Gram Staining‚ Fluid thioglycollate/aero-tolerance test‚ Methyl Red-Vogues Proskauer‚ SIM test‚ Lysine Decarboxylase test‚ Lactose Fermentation test‚ Simmons Citrate test‚ and TSI test. The Gram staining technique showed that the organism was Gram negative and bacilli shaped. The fluid thioglycollate test showed that the organism was a facultative anaerobe
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Questions 1. Describe the major differences between gram positive and gram negative bacteria cell walls. The gram negative bacteria cell wall is a thin peptidoglycan layer and an outer cell membrane with a lipopolysaccharide layer. The gram positive bacteria cell wall is a single thick peptidoglycan layer. This wall forms in a mesh like formation of three layers of alternating material. 2. From the procedure that you have carried out‚do you feel that the Gram positive stain is a simple procedure
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Observation The authors of this experiment‚ The Effects of some Inhibitors on the Rates of Photosynthesis and Respiration by Chlorella‚ knew that the rates of photosynthesis and respiration of many plants are affected by an adding chemicals. (Yaun and Daniels‚ 2000) The authors in this experiment would like to know in which ways different inhibitors affect photosynthesis and respiration distinctly. Objectives How certain inhibitors affect photosynthesis How certain inhibitors affect respiration
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KARYOTYPING Karyotyping refers to use of a microscope to examine the size‚ shape‚ And number of Chromosomes in a sample of body cells. Extra‚ missing‚ Or abnormal positions of chromosome pieces can cause problems with a persons growth‚ development‚ and body functions. In karyotyping‚ the chromosomes in a cell are stained with a dye to make the bands of each chromosome visible. Banding helps show differences in structure among the chromosomes and helps arrange them into pairs. The chromosomes
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Macromolecules Lab Purpose: to determine a method of testing for macromolecules. Materials: Knowns: Unknown: Test Solutions: Glucose solution Unknown solution Iodine solution Gelatin solution Benedict’s solution Starch solution Biuret solution Oil Brown paper Water Procedure: 1. Create a data table. 2. Label 5 test tubes with known solutions. 3. Add 10-20 drops of each known solution to respective test tubes‚ do not mix pipets! 4. Add 3-5 drops of
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Question 1 of 12 2.0 Points All of the following are considered microbes except A.viruses B.bacteria C.protozoa Correct D.worms Answer Key: D Question 2 of 12 2.0 Points Microbiologists investigate problems by designing and carrying out experiments. What is true about the scientific method? A.a hypothesis is the definitive explanation to account for the observation and therefore does not need to be tested B.a prediction is the factor that can change
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carried out in properly supervised manner to compare the unknown bacteria to the six known that were mentioned above. First it was the Gram staining procedure used to categorize bacteria in two groups‚ gram positive and gram negative. Gram staining is one of the most useful test used in the clinical setting to identify bacterial colonies due to their broad staining spectrum. (Black‚ 2008‚ pp. 70-71) The basis of the Gram stain is that gram positive bacteria retains the color of the primary dye‚ the
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further tests had to be conducted to fully confirm. Bacteria A showed a white opaque growth in both of its plates. Secondly both bacteria had to be gram stained‚ so that they could be identified as gram-positive or as gram-negative. The same gram staining method was used for both bacteria‚ which consisted of inoculating each bacteria into a
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