The methylene blue staining procedure is used to measure yeast viability based on the assumption that the methylene blue will enter the cells and be broken down by living yeast cells that produce the enzymes which breaks down methylene blue‚ leaving the cells colourless. The non- viable cells do not produce this enzyme (or enzymes) and as such the methylene blue that enters the cells are undegraded causing the cells to remain coloured (the oxidized form concentrates intracellularly). The coloured
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pathogens and their related epidemiology has become increasingly more important. The purpose of this study was to identify an unknown bacterium in a controlled laboratory environment over a 5 week period. Utilizing a variety of differential testing and staining methods learned in the microbiology course‚ students were to determine the identity of an assigned unknown organism. Observations were made and recorded each week to narrow down the scope of identification. Data has been presented in the tables
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Bacteria that Grow in Milk Jennifer Beagley and Katie Anderson Starr’s Mill High School 193 Panther Path Fayetteville‚ Georgia 30215 10th Grade Table of Contents 1. Abstract 15 2. Introduction 1-3 3. Materials 4 4. Procedures 5-6 5. Results 7 6. Discussion8-9 7. Conclusion 10 8. Acknowledgments 11 9. Bibliography 12 10. Appendix 13 11. Forms 14 Introduction The type of bacteria found in milk can help society in many ways. For example‚ if testing
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Pets as carriers of soil inhabitant microbes into our homes GIZELL VASQUEZ Biology department‚ Pathogenic microbiology‚ Crosby Jones‚ Angelo State University‚ San Angelo‚ TX 76909 There are approximately 78.2 million owned dogs in the United States (1)‚ and according to the United States government the current population of the nation is 308.7 million. The portion of dogs to humans is 4:1. These statistics are of importance because these pets physiologically observed their environment by smell
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smear my bacteria on a liquid medium. I then proceeded to incubate the medium for 24-48 hours. 1. GRAM STAIN The next step I took in finding my unknown bacteria was to gram stain it. This is used to differentiate the bacteria. The different staining reagents are: crystal violet‚ grams iodine‚ acetone-alcohol‚ and grams safranin. Under the microscope it was a pink color‚ which means Gram Negative. Also‚ the shape was a rod. 2. KLIGER’S IRON
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shapes would distort. BM SC Negative Stain CULTURES: Bacillus subtilus Staphylococcus epidermis MATERIALS: Nigrosin‚ Clean slide‚ Distilled water‚ Sterile toothpicks FUNCTION: This technique is very useful in situations where other staining techniques do not show clear morphology or size. PROCEDURE: 1. Place a small drop of nigrosin near on end of the slide. Mix a loopful of broth culture in the drop. (When from a solid medium‚ add a loopful of water) 2. Draw a second slide
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Microbiology Laboratory Report Identification of Unknown Bacteria 6O BIO 251-7H1 Gram Stain Summary: Out of 28 Unknown microorganisms‚ slant 6O was randomly selected‚ and subjected to testing in order to identify it’s species. Seventeen of the unknown bacteria were classified as Gram-negative bacilli: Corynebacterium pseudodiphtheriticum‚ Enterobacter aerogenes‚ Eschirichia coli‚ Flavobacter capsulatum‚ Helicobacter pylori‚ Klebsiella pneumonia‚ Klebsiella oxytoca‚ Proteus hauseri‚ Proteus
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Work Plan for Isolation‚ Purification‚ Identification and Starter Culture Activity of Lactoccocus lactis Submitted by: M.Usman Akram B.S. (Hons.) Dairy Technology mh.usman@hotmail.com Mobile : +923217773736 University of Veterinary and Animal Sciences‚ Ravi Campus Pattoki Lactoccocus lactis Classification: Scientific classification | Kingdom: | Bacteria | Division: | Firmicutes | Class: | Bacilli | Order: | Lactobacillales | Family: | Streptococcaceae | Genus:
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My dichotomous key was the “key” thing to finding my unknown out. After the gram staining of the gram negative‚ the O-F test was the first test to start my process off; it produced yellow through out both tubes. This meaning it is a fermentor of glucose and can be oxidative of glucose. It is also considered O-F because the mineral oil
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Gram staining is a technique that was invented by Hans Christian Gram in 1882 (Cantey & Doern‚ 2015). First‚ a drop of water was placed on a microscope slide. Using a loop and aseptic techniques (working by an open flame to prevent contamination‚ and sterilizing
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