Abstract: It is important to be able to identify pathogenic bacteria that may be causing harm. Tomato crops can be affected by several different pathogenic bacteria. By using Koch’s postulates‚ it was determined that Pseudomonas syringae was the bacteria causing rot. There are four criteria that must be met when using Koch’s postulates. They are that the organism must be fund in all infected‚ the organism must be isolated in pure culture then once reinnoculated in a healthy host‚ must cause the
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Having the ability to collect and identify unknown microorganisms is vital in health and medicine. This capability is important for a variety of reasons‚ such as knowing the causative agent of disease‚ knowing if the microorganism obtains any beneficial properties and knowing the correct microorganism to use to create a successful antibiotic. Implementing the experimental methods learned thus far in the microbiology laboratory allowed an unknown bacterium to be identified as a result of this study
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lutea‚ Pseudomonas fragi‚ Micrococcus luteus‚ Alcaligenes faecalis‚ Clostridium sporogenes‚ and Micrococcus roseus. There were several qualitative tests that could be conducted to determine the identity of the unknown species‚ for example‚ Gram staining‚ Fermentation‚ Catalase‚ Oxidase‚ Starch Hydrolysis‚ Litmus Milk‚ MOI medium‚ and the Gelatin Test. All tests and techniques used were performed in accordance with The Microbiology Lab Manual. Materials and Method: A stock broth culture of
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nanoparticles by Bacillus sp.: The microorganisms in the most contaminated toilet Sink were isolated by dilution technique. The dominant bacterial strains are identified as Bacillus sp.‚ on the basis of morphological and biochemical characteristics. Gram’s staining: Gram positive and rod shape. They were serially diluted and spread on nutrient agar plates. The starch has been hydrolyzed and a clear zone by the addition of iodine solution. Visual identification: When bacterial biomass mixed with silver nitrate
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Traditional Analysis of Unknown Colony Morphology Color- light yellow pigmentation Form- circular Elevation- convex Margin- entire [staphylococci] DNase Test This test is used to detect if the bacteria contains any deoxyribonuclease activity. Because no color change was observed from blue to clear my unknown bacteria displayed a negative result. Blood agar plate This test is used to detect the hemolytic activity in the bacteria. A darkish green color on the media around the
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UNKNOW BACTERIA LAB REPORT UNKNOWN 36 Introduction The purpose of this lab was to identify two unknown bacteria from a mixed culture. The reason for identification of unknown bacteria was to help students recognize different bacteria through different biochemical tests and characteristics. This is important in the medical field because identification of unknown bacteria can help treat a patient by knowing the contributing source of a disease. Also knowledge of different bacteria helped others
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Review for Microbiology Test #1 (Lesson 1-5) Lesson 1 What is the #1 killer of man worldwide? Heart Disease. What is the #3 killer in the US? Infectious Disease. What is the importance of MO in our world? List 6-8 reasons MO are important. Can’t live in Germ Free World‚ Keeps Economy running‚ Agriculture‚ Medication‚ Baking‚ Cosmetics‚ Paints‚ Fertilizers‚ Helps develop immune system‚ Decomposition of dead plants and animals to enrich the soil. What are the 4 groups of people most prone to
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Sarah Helms Thursday/ 11:15am #1 Escherichia coli There are many types of microorganisms and ways to treat each one. Knowing the differences of each is vital to treat a patient correctly. The purpose of this report is to explain the process and steps used to identify a certain microorganism referred to as the unknown. Materials and Methods A test tube with an unknown microorganism will be retrieved. Once the test tube is retrieved‚ a steak for isolation will be completed in order to produce
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appearance was observed and recorded. There was growth that appeared pinkish in color. The first test we did was basic stain using a heat fixed emulsion which kills the bacteria and allows them to adhere to the slide and thickens their protein for better staining. I then covered the heat fixed emulsion with crystal violet for one minute. The stain was then rinsed with distilled water. The stain was then pat dry with bibulous paper then observed under oil immersion to determine morphology. Upon completion
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to make a presumptive identification of the EI. The EI sample was taken from a door handle to the George Lynn Cross Hall‚ which is touched by hundreds of students entering the building every day. The most important exercises performed include gram staining‚ which
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