The plate count agar‚ CFC agar and cetrimide agar are used and provided different evidences on the isolation of Pseudomonas from the soil experiment. Firstly‚ the plate count agar is a medium for the enumeration of viable organisms in food‚ water‚ waste water and also from clinical samples‚ and thus it is non-selective to any species. Whereas the CFC agar is a selective agar which contains reduced amounts of cetrimide but also cephaloridine and fucidin are added to allow the isolation of most Pseudomonas
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The aseptically inoculate nutrient broth tubes A and B did not have any microbes inoculated into the tubes so it should not have been cloudy and that was what was seen. Tube C should have been cloudy because it had E. coli inoculated into the nutrient broth and that was also seen. A simple streak was also performed and it looks has if the loop went over previous streaks which should not have been done. A complex streak was also done. No colonies grew towards the end of the streak‚ this could be due
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Discussion After doing a series of different biochemical tests for the isolated organisim from the sputum sample‚ it was concluded that the unknown Gram positive organism was Streptococcus pyogenes. The sputum sample was first inoculated in the agar plates MAC‚ SBAP‚ CHOC and also a direct gram stain of the sample was performed. After a day of incubation‚ the MAC agar gave a negative result‚ therefore it is not a Gram-negative bacteria and non-lactose fermenting. The SBAP and CHOC showed growth
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POLHN Microbiology Student Name: SOKOPETI FAKAOSIFOLAU. Assessment 6 – Gram negative bacilli 1. Which of the following is not true of Enterobacteriaceae? a. Gram negative bacilli b. Oxidase positive c. Reduce Nitrate to Nitrite d. Ferment glucose with acid production 2. Which of the following organisms does not belong to the Enterobacteriaceae group? a. Shigella flexneri b. Eschericia coli c. Proteus mirabilis d. Pseudomonas aeruginosa 3. Name the four species of Shigella
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A Comparative Study of Dalandan and Kamias as Decolorizer in Gram’s Staining Staphylococcus aureus and Escherichia coli An Undergraduate Research Presented to The College of Medical Technology Centro Escolar University In Partial Fulfillment Of the Requirements of the Degree Bachelor of Science in Medical Technology By: Aduana‚ Frances Mae D. Barnachea‚ Erika Joy V. Decleto‚ Pearl Yvette P. Lagmay‚ Ira Alexis C. Paris‚ Dublin Justin Patron‚ Chronielle Albert C. Radovan‚ Denzel James C
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Microbiology Final exam Study reference 1. Gram Stain * Verify if bacteria are present or not. * Controls – positive (purple) – S.aureus negative ( red/pink) – E.coli 2. Endospore Stain Positive controller – B. magneterium Green spore- Positive Pink (vegetative ) – Negative 3. Acid fast Positive control – M. smeagmatis Blue – negative Pink /red- positive 4. Motility Positive control - P.vulgaris A. Non motile is negative test B. Motile is a positive test 5. Carbohydrates
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ASSAYING PLANT SUBSTANCES FOR ANTIBACTERIAL ACTIVITY PURPOSE To test plant extracts to see if they possess antibacterial activity. PROCEDURE First create a list of plant extracts being tested and choose 2 extracts per group. The plants being tested are Oregano‚ Tea tree‚ Eucalyptus and Colloidal Silver. Obtain two nutrient agar plates and using a permanent marker draw four quadrants on the bottom of each agar plate. Using a sterile pipet transfer 250 ml of E. coli broth to the middle of
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Bio Lab Report Title: Gram-Negative and Gram-Positive contamination in yogurt. Introduction: There is bacteria that is beneficial and harmful in the human. Yogurt was choosed to be the best example that contains this bacteria. What happens to be the difference between these two types of bacteria‚ and how are they harmful? The bacteria is considered “Gram-Negative‚” and the substance is considered contaminated when there is more negative bacteria than positive bacteria. This will prove or disapprove
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University of Phoenix Material Appendix B – Identifying Bacteria Bacteria identification is accomplished in a number of ways. Two common tools microbiologists use to identify unknown bacteria include dichotomous key and biochemical tests. The dichotomous key is useful when a microbiologist only needs to know which group an unknown microbe belongs to on a general level. When a microbiologist needs to identify a specific bacterium‚ biochemical tests are used. PART ONE: GENERAL BACTERIA IDENTIFICATION
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Mystery bacterium I would not say science is storybook fun‚ but who knew it become a mystery. Trying to find out what was in our number seven vial would become a battle we were willing to take on. As I began the test of deciding if our little bacteria friend was gram positive or negative‚ Jordan my science teammate‚ was putting together a smear plate. In as little as ten minutes we had discovered by the pink oval shapes we were observing‚ our microbe friend was a gram negative rod. We had narrowed
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