To fully understand this lab we first need to know what gram-negative and gram-positive bacteria are and what it’s composed of. Gram-positive bacteria take up the class of bacteria that stain crystal violet in the method of bacterial differentiation. Where gram-negative bacteria are a class of bacteria that does not retain crystal violet. Instead they are counter-stained pink by Safranin‚ and because of this‚ identifying positive is possible. One of the many differences between gram-negative and
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Gram Stain: Gram is an empirical method to distinguish the species of bacteria into two groups Gram-positive and Gram-negative based on the physicochemical properties of the cells. First‚ a smear was prepared by use a sterile transfer loop that been flamed to removes some bacteria from slant agar and placed on the slide; mixed with one drop of water and let air dried. After dried‚ heat fixation the slide by passed the slide over a flame quickly 2-3 times to stick bacteria to the slide. Next‚ the
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Bacteria Morphology April 2‚ 2015 Abstract – The Purpose of this exercise is to gain experience in bacterial morphologies in prepared wet-mounted slides and interpreting the findings of bacteria through direct and indirect staining technique. Hypothesis – The experiment will allow for further insight into stained organisms‚ allowing extended contrast‚ differentiating shape and structure through utilization of microscope. Procedure – Instructions followed as per procedure‚ specimens
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belts‚ caps‚ etc‚ are the most preferred clothing for today’s youth. This study discusses compositions and methods of reducing or preventing back staining of indigo dyes on the weft yarn and pocket of denim garments. The dye‚ as it is removed from the denim material‚ post treatment with cellulase or by a conventional washing process may cause ‘back staining’ or ‘redeposition’ on the denim material; e.g : re-coloration of blue threads and blue coloration of white threads‚ resulting in less contrast between
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| Gram Negative Unknown | Biology 3444-006 | | Lena Wallace | 11/7/2011 | | Abstract: The purpose of this lab was to identify an unknown bacteria culture using differential tests. The identification of the unknown culture was accomplished by identifying the bacteria based on its specific metabolic characteristics and morphology. It is suggested that culture 11 is a sample of Enterobacter aerogenes. Introduction: This experiment was centered on metabolic and biochemical testing
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3.1. Isolation and identification of Bacterial isolates An enrichment culture technique was used for the isolation of bacteria responsible for biodegradation of phorate in soil. Screening of these bacterial species for phorate degradation in liquid cultures in our previous study (Jariyal et al.‚ 2014)‚ resulted in identification of bacterial species B. aerophilus strain Imbl 4.1 ‚ Brevibacterium frigoritolerans strain Imbl 2.1 and Pseudomonas fulva strain Imbl 5.1. However‚ these bacterial species
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The pGLO lab is a lab where students attempt to put the genes that make a jelly fish glow into E. Coli. After a process called transformation‚ the process in which a cell takes up and expresses a new piece of genetic information‚ the E. Coli will be able to glow and will be antibiotic resistant. The students first need to learn a couple of techniques before they are able to begin this lab. The first technique they will need is how to keep their environment sterile. They must learn to only open their
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1. Make sure you label both plates on the bottom‚ with your name‚ date and name of pathogen you have chosen (listed above under materials) Then label numbers 1-5 on one plate and 6-10 on the other as there are 10 disks that need to be placed. Spread out in a circle formation like pictured below (This is the final product; look at this for reference on how to label) Now you are ready to start culturing. Start with one of your plates you can do both at the same time but to avoid making any errors
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Lakeli is absolutely stunning‚ but for his beauty he has paid the price. He is a beautiful 12 week old deaf Great Dane. While we all think white Great Danes are stunning most of them are going to be either blind‚ deaf‚ and occasionally both. What is a Double Merle and how are they created? A few things to keep in mind about the Merle pattern as you read. •Merle is a pattern not a color. •Merle is a bleaching pattern. •The Merle gene creates mottled patches of color in a solid color coat‚
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The tube that contained my unknown material was tube number twenty. I did a gram stain‚ oxidase test‚ and used three different medias to narrow down and identify my unknown organism. The first step I took in identifying was a gram stain. This gram stain would reveal whether my organism is gram positive or gram negative which would narrow my choices from fifteen options down to six. After completing the gram stain‚ my unknown was the color pink. This revealed my unknown as being gram negative.
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