1. Title: Double unknown identification of a mixed culture of Gram-negative and Gram-positive bacteria 2. Author: Nick Fiore‚ University of Kansas‚ Biology 402‚ Fall 2014 3:00pm room 6040 3. Abstract: The purpose of this experiment was to isolate two unknown bacteria and perform a series of selective and differential tests to correctly identify each. After the bacteria was isolated a series of differential and selective tests following the dichotomous key attached were used to identify each bacteria
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Unknown Experiment Introduction: The purpose of this experiment is to distinguish and indentify an unknown bacterium. There are several tests that can help one eliminate and narrow down the options. The most useful test‚ and the very first one done‚ is a gram stain. This test will tell whether the bacterium is gram-positive or gram-negative. After the type of gram stain is identified‚ the tester has a wide array of differentiating tests at their disposal. Based on the results from these
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Isolation of Bacteria in our Physical Environment Introduction: The purpose of the lab is to apply what has been learned so far in Microbiology‚ and use it to collect and examine microbial species from the physical environment. The physical environment around the lab will be utilized to find out the variety of different microbial species growing in and around the lab. This investigation will help in preparation for the individual projects that will be done at the end of the semester. Materials
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I was able to come to the conclusion that my unknown is Klebsiella pneumoniae through the use of Bergey’s Manual. After performing a gram stain of my unknown I was able to determine that my unknown was a gram-negative rod. While testing the oxygen requirements my test tube had growth throughout the tube‚ indicating my unknown was a facultative anaerobe‚ which can grow in the presence or absence of oxygen. The most important test was whether my unknown was motile or nonmotile. My test tube only showed
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Lab Report: Effects of Lysozyme Introduction: This report discusses an experiment that was done to demonstrate the effects of lysozyme on populations of Gram positive and Gram negative bacteria. Bacteria have a cell wall composed of peptidoglycan that gives the wall its strength. Gram negative bacteria have and extra component of lipopolysaccharide (LPS)‚ that is stabilized with magnesium ions‚ to their cell wall that further protects them. When Gram positive bacteria are treated with lysozyme
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The purpose of this exercise was to use our skills we have learned in the lab to identify two unknown organisms. PROCEDURE I was given unknown #76. I performed the streak plating method with my unknown organisms. To perform this exercise I needed a TSA plate and labeled the bottom of it with my name‚ group number and organism. I also divided the bottom into three sections. After that‚ I sterilized my inoculating loop using the bunsen burner flame. After letting the loop cool‚ I reached into my
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MICROBIOLOGY 3444-007 Gram Negative Unknown Unknown Bacteria #14 LeNaiya Kydd 4/2/2014 Abstract In order to be able to identify the unknown organism that was given to us‚ we had to conduct a number of different tests. These biological tests are used because they help us be able to identify the properties of the unknown we have and be able to compare our observed results with actual results of all the potential organisms. When all of the data of the test are put together it is easy to
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Unable to read the Gram Stain at first it was later confirmed that Unknown #7 was a gram negative bacteria. When introduced to Starch Unknown #7 produced no zone of clearing showing that it did not contain the enzyme amylase. When introduced to the Lipid Unknown #7 did produce a zone of clearing indicating that it did contain the enzyme lipase. When introduced to Casein Unknown #7 produced no zone of clearing indicating that it did not contain the enzyme protease. When streaked onto the Gelatin‚
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cell wall‚ with a large amount of peptidoglycan. Peptidoglycan is a polymer composed of modified sugars cross-linked by short polypeptides. In effect‚ the cell wall traps the crystal violet in the cytoplasm‚ concealing the red dye safranin in gram staining experiment. Certain gram positive species have virulent strains that are resistant to one or more drugs. Gram negative bacteria on the other hand are composed of lipopolysaccharides (LPS)‚ porin channels‚ and murein lipoprotein which is all absent
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performed within a certain time frame the specimen will become contaminated. Part II: Bacterial Anatomy The purpose of this experiment is to become familiar and gain an understanding of how morphology and the arrangement of bacteria relate to staining techniques. Sample 1 In this study a female patient who had a problem that had arisen suddenly after lunch‚ had allergic reaction to something that she ate or drank had sent her to the bathroom with cramps and copious diarrhea. She knew she
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