types of bacteria? Gram Stain This separation is based on differences in what physical trait? The structure of the cell walls. What is the iodine used for in this staining technique? Crystal violet is first used to stain the sample and iodine is used to affix it. Why is it important to heat fix the bacteria prior to the staining procedure? This procedure is done to kill any bacteria that may be on the slide already and this will insure a clean slide. If you had a bacteria sample that you knew
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When I received my slant culture‚ I immediately made a streak plate and started the Gram Stain protocol1. In the first two slides‚ I made and stained‚ my results were ambiguous. Without putting the slide into the microscope‚ I noticed one was pink and the other purple. To resolve it‚ I discarded my slides and started again and was extremely careful in proceeding the Gram Stain. I concluded that my unknown A was gram-negative bacilli. Using the dichotomous key for gram-negative bacilli I started
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In the unknown identification labs‚ we have identified our unknown as Pseudomonas aeruginosa. Pseudomonas aeruginosa is Gram negative and rod shaped that we found to be motile in the lab. Our strain of P. aeruginosa formed colonies that were round in shape and had scalloped margins on nutrient agar. On our agar slant‚ the P. aeruginosa colonies had a filiform appearance on the edges. I think we correctly identified our unknown as P. aeruginosa because we performed several different tests‚ eleven
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Our purpose for this experiment is to observe how well organisms grow on three different media types such as chemical defined‚ complex‚ selective‚ and differential media. Experimental Methods In this lab‚ we use the procedure from Chemically Defined‚ Complex‚ Selective‚ and Differential Media. We divide the agar plates that have the unknown in third‚ write our initials‚ and date on the bottom of the agar plate. We also divide the agar plates into quadrants‚ label each organism in their quadrant
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The catalase test is used to differentiate staphylococci (catalase-positive) from streptococci (catalase-negative). The enzyme‚ catalase‚ protects the bacteria from the toxic by-products of oxygen metabolism. This enzyme is produced by bacteria that respires using oxygen. The catalase-positive bacteria include strict aerobes. Catalase-negative bacteria may be anaerobes‚ or they may be facultative anaerobes do not respire using oxygen as a terminal electron acceptor. The test reaction is very fast
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A streak culture was performed on a Tryptic Soy Agar Plate for isolation and purity and then incubated at 37 degrees Celsius for 24 hours. A gram stain was performed to determine whether the bacterium was a gram negative or a gram positive. After performing the gram stain‚ I concluded that by the appearance of purple spherical clusters resembling grapes that is was a gram positive cocci. A Catalase test was then performed using hydrogen peroxide. A positive catalase test was observed
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Pseudomonas aeruginosa Pseudomonas aeruginosa is the bacteria of my initial unknown project. Determining which bacteria I had was completed by many steps. The one fact that I had‚ was that it was a gram negative bacteria because on the gram stain it appeared pink. Its cell wall is composed of a plasma membrane‚ periplasmic space‚ peptidoglycan and an outer membrane (lipopolysaccharide and protein). By looking at the agar plate it was easy to tell that it was not a swarmer and it did not have any
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Phelps‚ Biology 205 MW 2:10pm. Unknown Organism Paper The unknown organism tested in the Mesa lab was collected from in between the toes of my puppy‚ Riley. The original culture grown consisted of dozens of visibly different organisms of varying colony colors and growth patterns. I chose to test a smaller‚ red colony for my experiments. Based on its gram reaction and oxygen requirements‚ the following tests were performed to reach my presumptive ID: Test Performed Result Gram Stain +
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Gram Negative Unknown Lab Report Abstract The Unknown Gram Negative bacterium inoculated in a Tryptic Soy broth medium was randomly selected from a group of other unknowns. In order to identify this unknown the seven different types of biochemical tests will be conducted on this unknown bacterium to identify it out of 6 possible bacteria; Escherichia coli(E. coli)‚ Enterobacter aerogenes (E.aerogenes)‚ Klebsiella pneumoniae (K.pneumoniae)‚ Proteus mirabilis(P.miranilis)‚ Pseudomonas aeruginosa(P
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The first test conducted on unknown bacteria 32 was the Gram stain. From this stain‚ unknown 32 was found to be a Gram-positive cocci. This test eliminated all possible Gram-negative bacteria‚ Gram-positive rods and Gram-positive spirillium. Next‚ the endospore test determined whether or not the Gram-positive bacteria contained endospores. With the use of malachite green‚ steam‚ and safranin it was found that unknown bacteria 32 did not contain endospores. This eliminated Gram-positive cocci Sporosarcina
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