As a result of the tests ran‚ I was able to identify the unknown bacteria. For Unknown A‚ I ran six tests. I first isolated the bacterium from the second bacterium and found a clear growth (Table 1‚ Figure 1). Secondly‚ I ran a gram stain and found a gram positive‚ cocci bacterium (Table 1‚ Figure 2). Third‚ I ran a catalase test in which was negative (Table 1). From here‚ I determined a starch hydrolysis test would be necessary to distinguish between different bacteria. The result was negative (Table
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trait? The separation is based on differences in the structure of the cell walls What is the iodine used for in this staining technique? The iodine in this staining technique is used after the crystal violet is applied. The iodine then affixes this and then a decolorizer is applied to take away the primary stain. Why is it important to heat fix the bacteria prior to the staining procedure? It is
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The Role of Agarase in Agar-Degrading Bacteria Abstract Agar-Degrading (agarolytic) Bacteria is physiological class of bacteria capable of utilising agar as a sole carbon source. This ability is made available by the use of agarases - enzymes which break down agarose into oligosaccharides. This physiological class branches through genii‚ regardless of Gram Stain status or morphology. Through a review of scientific literature we can find identification methods‚ optimum conditions and the
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bacteria need to be separated and isolated before they can be identified. Moreover‚ gram staining was used to understand the basic morphology of these bacteria. As these plates were incubated and grown‚ the presence of two separate bacteria colonies was visible. The colonies from the mannitol salt were used to incubate a TSB broth to grow the gram-‐positive culture. The purity of this broth was tested using gram-‐staining technique. A circular colony from the TSA plate was used to incubate a TSB broth
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Title: Confirming the Presence of Fermentation in the Conversion of Milk to Kefir Authors: Michael Ahrens‚ Nicholas Fiore‚ Garrett Hages‚ Melissa Cullom University of Kansas‚ Biol 402‚ Fall 2014‚ 3:00pm room 6040 Abstract: In this experiment milk was fermented into Kefir‚ then a series of tests were used to confirm that fermentation actually occurred. The tests used to confirm the fermentation were a gas production test‚ pH test‚ Gram stain‚ and turbidity test. The results showed during the conversion
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[either flexneri or sonnei‚ we used both in our lab during the semester]. Procedure {and observations}: Observe bacterial colony morphology. {Colonies are large‚ beige or cream-colored‚ with irregular borders.} Prepare two slides for gram staining and viewing under a microscope. {Either my gram-stain slides were bad or the microscopes I chose for viewing were not good. No bacteria were found under the microscope.} Prepare another gram stain of the unknown bacteria. {Gram-positive‚ coccus
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differential tests. The tests used to identify the unknown bacterial culture included: Gram stain‚ mannitol salt agar‚ coagulase tube test‚ and an antimicrobial susceptibility test. The tests selected were based on the results of a gram stain. Gram staining‚ the most commonly used differential stain‚ allows for the fast and easy detection between gram negative and gram positive organisms based on the presence or absence of peptidoglycan. A gram positive organism resists the decolorization process due
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1. What are the advantages and disadvantages of the different types of light and electron microscopes discussed in Chapter 3 that are used to study microorganisms? Focus your response in terms of the following parameters The disadvantage of using a light microscope is its inability to magnify a specimen as great as an electron microscope. An electron microscope can magnify up to 500‚000 times‚ whereas‚ a light microscope can only magnify an object up to 2000 times. The resolving power or resolving
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learned so far in the microbiology laboratory class for the identification of an unknown bacterium which I was using unknown #25. Results Unknown #25 had the following morphology on a streak plate: medium sized butyrous cream colored colony. Gram-staining was utilized as a first step to differentiate between gram-positive and gram-negative. After determining that it was a Gram negative single bacillus‚ biochemical tests performed and their purpose and results are shown in table and flow chart form
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Exercise 5 Introduction: Many different species of bacteria look similar under the microscope and also have the same staining results (ex. Gram stain). To be able to differentiate between the different species‚ one can look at the metabolic differences (fermentation)‚ as well as the environmental condition differences (temperature‚ pH‚ oxygen requirements). Being able to manipulate these conditions in a controlled environment can help to correctly identify the exact bacteria. Different media
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