one to identify distinctive aspects of the bacteria. Some examples of these tests are a gram-stain and an API test. These tests allow one to figure out what properties a bacteria strain contains‚ such as gram negative or gram positive with the gram staining test‚ or what bacteria an unknown is based on positives and negative of the API test. The results of API tests‚ will show extremely accurately what a bacteria is based on previous tests performed. One such bacteria than can be identified is Salmonella
Premium Microbiology Bacteria Gram staining
Method • Methodology is performed through 3 successive days: First Day: Trypan Blue cell viability assay: Description Trypan blue staining is a simple way to evaluate cell membrane integrity (and thus assume cell proliferation or death) but the method is not sensitive and cannot be adapted for high through put screening. Short 96 well assay: EACH condition should be done in triplicate or more. Steps 1. Trypsinize one T-25 flask and add 5 ml of complete media to trypsinized cells. Centrifuge
Premium Cell culture Cell Bacteria
TITLE Lab #4 Soil –vs- Water Microbiology INTRODUCTION There are 4 learning goals for this lab: 1. Collect samples and compile data from at least 2 sources (soil; water) of your choice. 2. Identify at least 2 Prokaryotes (bacteria) and 2 Eukaryotes (fungus) in your samples. 3. Research the importance of bacteria and fungus to Earth in a non-medical context. 4. Compile a high-quality‚ 3+ resource (excluding the lab worksheet)‚ MLA-cited in-text laboratory report using the scientific method
Premium Gram staining Bacteria Fungus
Unknown Lab Report Unknown Organism #6 Ann Le (Phuoc) May 6‚ 2010 Dr. Carrington Microbiology Lab- MW 12:50 Le 1 I. Introduction My unknown organism #6 is Morganella morganii‚ which is a gram-negative bacillus rods commonly found in the environment and also in the intestinal tracts of humans‚ mammals‚ and reptiles as a normal flora. (3‚ 5) This bacterium Morganella morganii‚ was first discovered in the 1906 by a British bacteriologist named H. de R. Morgan. (2) Despite its wide
Premium Hydrogen sulfide Gram staining Agar plate
a practice that aids in Increasing ones knowledge of a laboratory setting and it helps improve on Using sterile technique. Isolates of soil microbes can be categorized and Characterized based on a number of criteria ranging from gram-staining Which is done for this project to enumeration which is quantitative description Based on the amount of microbial colonies available. The Antimicrobial Activity of three different microbes were also tested and results were obtained
Premium Bacteria Microorganism Microbiology
Requirements for Unknown Report You have been provided a mixed culture (a gram positive and a gram negative) from which you should have isolated each type of organism‚ described their colonies on a TSA plate‚ and described their cellular morphology. You were graded on this activity separately. After you have isolated your gram negative in pure culture you will be subjecting it to various biochemical tests and making observations about the cells‚ colonies‚ growth temperature preferences‚ etc. in
Premium Escherichia coli Gram staining
iLab #2 Carbohydrate Tests INTRODUCTION - For this experiment we will be testing four different bacteria with four different tests‚ using glucose‚ lactose‚ and sucrose. Hopefully we will use the information from those test to be able to identify the organisms in each of the samples from the case studies. We will use the results from the four different tests along with the information of how different bacteria react to match up to the case
Premium Metabolism Bacteria Sugar
04/29/2013 G-Unknown code Gram Positive-Micrococcus luteus Gram Negative- Klebsiella pneumoniae Unknown Paper The unknown project was a very good realization of me and my partner going out by our selves. The very first day of the Unknown project I was acquainted with Denise who was very friendly and was very nice as far as assisting me in the project. The first day we were introduced too various different forms of the unknown such as broth‚ Blood agar plate‚ MSA plate
Premium Gram staining Agar plate
Antony R 9:00Am-11Am Unknown Number 94 Unknown Results Streptococcus salivarius The first step in my unknown identification was to carry out a gram stain to observe the cell shape and arrangement. My unknown turned out to be gram positive bacteria cocci shaped with long chain cell arrangement. The unknown was gram positive because the cells stained blue-violet ‚this indicate that my unknown has ability to retain crystal violet-iodine after alcohol application on the cells which is a gram
Premium Bacteria Streptococcus Agar plate
UNKNOWN LAB REPORT UNKNOWN NUMBER 54 Ron Williams 7-28-05 Bio 205 Prof. Curlee Fall 2005 Purpose There are many reasons for knowing the identity of microorganisms. The reasons range from the knowing the causative agent of a disease in a patient‚ so as to know how it can be treated‚ to knowing the correct microorganism to be used for making certain foods or antibiotics. This study was done by applying all of the methods that have been learned so far in the microbiology laboratory
Premium Bacteria Enzyme Glucose