EXPERIMENT 1: PREPARATION OF BIOLOGICAL MATERIALS FOR MICROSCOPIC EXAMINATION Introduction: Cell is the smallest structural and functional unit of an organism‚ and is often called the building blocks of life. All organisms are composed of one or more cells‚ they are classified into 2 groups which are unicellular organisms and multicellular organisms. Plants and animals are made up of cells too. There are two basic classes of cells‚ prokaryotic and eukaryotic cells. The structurally simpler‚ prokaryotic
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giant cluster tightly packed together. 4. What is the difference between direct and indirect staining? (1) Direct staining stained bacteria with basic dye (methylene blue‚ carbol fuchsin‚ crystal violet‚ etc) which its coloring power is in positive ion‚ and reacts with the negatively charged cell. Thus‚ the bacterial cell will be stained with the basic dye’s color. In other words‚ direct staining
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lab an analyzed under a microscope. There are three suspect bacteria‚ which are Bacillus‚ Mycoplasma‚ and Escherichia. A discussion of staining procedures‚ as well as anatomical differences will be discussed for each specimen. Bacillus are Prokaryotic cells‚ that are rod shaped bacteria and can from long chains. Bacillus are also spore forming. When gram staining a Bacillus sample‚ they are found to be gram positive. However‚ there are some Bacillus that are gram negative. Escherichia are similar
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sputum sample and will be testing for one of the following genera: Bacillus‚ Escherichia and Mycoplasma. In order to determine which one of the three may be the source of the infection we have to recognize the characteristics of each one and the staining protocol needed to identify them. All three of the above mentioned genera are considered to be members of the Prokaryotic cell family which includes bacteria. They have certain identifiable features that distinguishes them from Eukaryotes such as:
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PTK7 Regulates Epididymal Duct Coiling Through Regulating Myosin II Activity in the Surrounding Mesenchyme Cells of the Epididymal Duct. By Angela Washington Research under the supervision of Dr. Barry T. Hinton of the Department of Cell Biology at the University of Virginia Health Systems Summer Session III 2012 Abstract It is very clear that the epididymis plays a crucial role in the maturation of spermatozoa and without a fully developed and functional epididymis male infertility will
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become directly stained. In direct staining‚ the organisms must be fixed by a process such as heat. Fixing the slide prevents the organism form washing off the slide before visualization. This is accomplished by passing a smear of the bacteria through flame. The heat sets the proteins of the organism thus causing the bacteria to attach to the slide. The organism can become damaged from the setting process and the use of heat prior to staining. In indirect staining‚ the negatively charged colored portion
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as per usage. NEED FOR TESTING •To estimate the quality parameters •To standardize •To meet company standards •To adhere to customer requirements Gray Scales It is a scale graded up to ‘5’ used for evaluating change in color and staining. The specimens are manually evaluated on a 5-4-3-2-1 scale where class 5 means no shade change and class 1 means a very great shade change. An average of rating is taken as a result. Intermediate ratings like class like 3.5 or
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This was compleated by growing the bacteria on a petri dish with agar‚ gram staining‚ then examning under a microscope. The bacteria identified was M. Roseus and B. Megaterium. The identity of these bacteria was mostly determined by the positve gram stain results and the shape they where under the microscope. Both of these bacteria
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Notes for WK2 – 10 September Lesson 1 * State the resolution and magnification that can be achieved by an electron microscope. * explain the need for staining samples for use in electron microscopy Lesson 2 * calculate linear magnification of an image such as photomicrograph or electron micrograph Key words * Resolution= the ability to distinguish 2 separate points as distinct from each other. * Magnification= the number of times greater an image is than the actual object
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Background: Lipid and protein composition varies in tissue types. Results: When comparing liver‚ muscle‚ and adipose tissue‚ adipose tissue had the highest percent lipid and liver tissue had the highest percent protein. Conclusion: Based on analysis of the gels‚ the liver appeared to have the most variety in proteins when comparing liver‚ muscle‚ and adipose tissue. Significance: In order to determine the composition of a tissue‚ specific macromolecules can be extracted‚ quantified‚ and analyzed
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