ABSTRACT The exercise was about the techniques in proper sectioning of plant tissues and staining techniques so that the study of plant structure will be easier. In free hand sectioning‚ the plant organ must be 45° upright and is held between the thumb and the base of the forefinger. A razor blade was used to cut the plant thinly in cross sections. Obliquely cut tissue is a big mistake since the surface of the tissue will not be even upon viewing under the microscope. Iodine-Potassium-Iodide
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surface stains‚ where the staining substance is spilled out onto the surface or material and is trapped in the fibers‚ pores‚ indentations or other capillary structure of that surface. The material that is trapped coats the underlying material‚ and the stain reflects back light according to it’s own color. Applying paint‚ spilled food‚ and wood stains are of this nature. A secondary method of stain involves a chemical or molecular reaction between the material and the staining material. Many types
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I am writing this letter to give my highest possible recommendation for Ms. Adebimpe Adelaja. I am Professor and Vice Chair for Strategic Technologies in the Department of Pathology and Laboratory Medicine‚ UC Davis. I had the opportunity to get to know Adebimpe when I served as a mentor for the Hugh Edmonson Summer Internship. On the first day of the internship‚ I was able to have a discussion with each student and select the student I would work with for the next 8 weeks. Adebimpe distinguished
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section interval‚ floated on the hot water bath at 500 centigrade for straightening and then picked up on the egg albumin (Mayer’s albumin) coated glass slides. The slides were allowed to dry in the warm plate at 400 C for 2 hours and stored. III.7 Staining Protocol: Deparaffinization: Slides with paraffin section were deparaffinized in xylene for 15 minutes (2-changes). Hydration did with descending grades of alcohol for 5 minutes each as
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made its identity obscured to be the diagnosed at the time when it was first discovered. This was because MTB was neither Gram negative or positive when it was Gram stained. Finally‚ the scientists employed different way of staining called acid-fast staining. Acid fast staining This technique allowed the scientists to diagnose the MTB. However‚ its treatment still remains somewhat problematic despite of its effective antibiotic treatment. This is because it requires a long and multiple antibiotics
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was able to correlate a specific pathogen caused a specific disease. We use his postulates because‚ if followed‚ they provide accurate data. 2. In 1884‚ Hans Christian Gram described a method of staining bacterial cells while not staining surrounding animal tissues; however‚ he thought the staining method he developed was faulty because not all bacteria stained. In a letter to the editor of the journal in which Gram published his findings‚ write your response to Gram’s concern. 3. Compare
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distinct edges. Part 4: Direct Staining: Slide One: There were two clusters that were fairly easy to recognize. All of the cells were cocci. Some of the cells were large while others were practically nonexistent. Slide Two: There were different layers of cells. The cells were rectangular in shape and varied in size. A nucleus was visible in each cell. Slide Three: This slide was a mixture of different shapes. Nothing was recognizable. Part 5: Indirect Staining: Chains of both cocci and
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Lab 4 – The Cell Answer Key Procedure 4.3 Draw a picture of a single Elodea cell and label all visible structures. See the diagram in your lab manual. Without staining‚ the only structures that should have been clearly visible should have been the cell walls and the green chloroplasts. Is this cell prokaryotic or eukaryotic? ______Eukaryotic_______________________ What evidence do you have to support this claim? The presence of organelles (chloroplasts) and its large size.
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the experiment to test this question is coffee‚ Coke‚ tea. Some people have more stained teeth then others‚ and that is affected by their diets. Different liquids can to different things to your teeth‚ and the most frequent thing done to teeth is staining. The reason for this project is to test what different liquids stain the teeth the most‚ so that people can prevent their teeth standing by not drinking as much of certain liquids. The next most common type of tooth stain is from a persons
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the size (length of the DNA molecule) of the product. Materials needed: Agarose TAE Buffer 6X Sample Loading Buffer DNA ladder standard Electrophoresis chamber Power supply Gel casting tray and combs DNA stain Staining tray Gloves Pipette and tips Recipes: TAE Buffer 4.84 g Tris Base 1.14 ml Glacial Acetic Acid 2 ml 0.5M EDTA (pH 8.0) - bring
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