SH Lymphatic structures and organs | Slide: Blood‚ humanStain/colour: H&ELink: http://vslide.med.unsw.edu.au/flashslide.jsp?fn=aacr_b33.svs&mag=80 Prominent features: * Identify the different kinds of blood cells. * Note the large number of RBCs compared to WBCs –doughnut shaped‚ no nuclei. Use as “measuring stick” – 7 microns. * Platelets are much smaller – purple fragments. * Monocytes: large‚ bean-shaped nuclei * Neutrophils: small granules in cytoplasm‚ multi-lobed
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There are two major steps that affect the sensitivity and specificity of AQD-Tn mAb probe: blocking time and staining time. First‚ blocking time was investigated. Different time periods were examined and it was narrowed down to 15minutes as the sufficient blocking time. Smaller time intervals were studied to further shorten the blocking time. As shown in Figure
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were then co-incubated with different nanoparticles for 4h. After removing the culture medium‚ the cells were incubated with a diluted DCFH-DA reactive oxygen species (ROS) probe for 20 minutes and observed under a microscope after washing away the staining solution. Evaluation of in vitro therapeutic effect of human tracheal epithelial cells‚ HTEpiC‚ were procured from the Cell Bank of the Chinese Academy of Sciences in Shanghai. These cells were grown in a specialized culture medium and exposed to
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Observation of mitosis in garlic root tips Abstract The purpose of this experiment was to establish the different stages of mitosis which occur in the tip of a garlic root. The root tip which was used had already been pre-stained with an aceto-orecein stain. The stain made the root tips more visible so that under a light microscope it would be much easier to distinguish where the root tip was. While under the light microscope the objective was to be able to identify and distinguish each of the stages
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bioreactive has been producing the more favorable results‚ and as in this figure bioreactive shows better results as expected. 8a. Summarize the results from figure 4 in your own words. During the 14-day measurement the MCS’s cells show more staining for Annexin V in the static lungs than the bioreactive as well increased PCNA for the bioreactive lungs. C10 contained higher levels of Annexin V during the 11-day measurement for static lungs‚ less were present in the bioreactive lungs. 8b.
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bacillus or rod shaped and found in probiotics • Spirillum Pulli is spirillum or spiral shaped‚ and evident in rat bite fever Exercise 3: Gram stain What is the function of the iodine solution in the Gram stain? If it were omitted‚ how would staining results be affected? Omitting the iodine in the gram’s stain would mean‚ the crystal violet that goes into the cell would be incapable of forming the Crystal Violet Iodine (CVI) composite. The CVI complex molecule possesses a bigger size than that
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If there is any update‚ it will be posted no later than this Saturday (2/8/14) Identify the following individuals’ major contributions to science Ehrlich – magic bullet Hooke –cell theory and microscope Jenner - cowpox Lister- disinfectant (phenol) Koch- kochs postulate Lancefield- immunology to classify via serotypes Linnaeus- nomenclature Pasteur- pasteurization‚ vaccine‚ microbes in nonliving matter‚ aseptic techniques‚ fermentation. van Leeuwenhoek- described live organisms with Hooke’s microscope
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‘slice extremely thin sections for viewing under the microscopes’ as Robert Hooke found that cutting a cork in a thin section gets a clearer view. As technological advances advanced new techniques also developed over time for example staining techniques. Staining techniques was developed to view and understand tissues under the microscope by using dyes to stain tissue and visualise
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1. The final yield of the cells can be determined by use of an automated cell counter or by use of a hemocytometer (Neubauer chamber). In our laboratory‚ we determine the number of purified cells in a Neubauer-improved‚ bright line chamber with V-slash with a depth of 0.1 mm and a counting area of 0.0025 mm2 from Marienfeld (Lauda Königshofen‚ Germany‚ PN # 0650030). 2. For determination of the final cell yield‚ mix a 10 µl aliquot of the purified cells (Figure 9D) with 10 µl of trypan blue solution
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that lens‚ do you know the difference between “magnification” and “resolution” power? Magnification: Makes image bigger to see detail. Resolution: Clarity of an object‚ resolution is more important than magnification. You have learnt many staining techniques through the lab exercises: What dye did you use in simple stain? Crystal Violet What dyes did you used in Gram stain? Crystal violet‚ gram’s iodine‚ and safranin violet. Why did you have to heat up the slide when you did the acid
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