Kuroda et al. Skeletal Muscle 2013‚ 3:5 http://www.skeletalmusclejournal.com/content/3/1/5 Skeletal Muscle Open Access RESEARCH Canonical Wnt signaling induces BMP-4 to specify slow myofibrogenesis of fetal myoblasts Kazuki Kuroda1‚4‚ Shihuan Kuang1‚2‚ Makoto M Taketo3 and Michael A Rudnicki1* Abstract Background: The Wnts are secreted proteins that play important roles in skeletal myogenesis‚ muscle fiber type diversification‚ neuromuscular junction formation and muscle stem cell function
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Laboratory 8 - Endocrine System Endocrine Gland Histology PhysioEX 9.0 – Endocrine System Physiology (Exercise 4) Endocrine Gland Histology Objectives 1. To understand the function of the endocrine system 2. To learn about the major endocrine glands‚ the hormones they produce‚ and the effects they initiate 3. To familiarize the student with histological identification of various endocrine glands Background The endocrine system consists of cells and tissues that secrete chemical
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of bacterium that allows it to survive poor environmental conditions are called endospores. Their tough outer covering is made of keratin and is also the reason they are resistant to heat and chemicals. The strength of keratin also makes it resist staining. Due to this drastic measures like steaming the bacterial emulsion is used to force primary stains such as malachite green into endospores in the Schrieffer Fulton method. This stain can be left on the slide for 15 minutes or more to stain the spore
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2013). Gram positive bacteria occupy a multilayered thick peptidoglycan‚ and teichoic acid. Teichoic acids assist with distinguishes gram positive bacteria from other bacteria. The Gram positive bacteria are easier to kill. The gram reaction of dye staining is blue or violet. Gram-negative bacteria cell wall can be described as having a thin peptidoglycan‚ no teichoic acid but it does have an outer membrane lipopolysaccharide‚ lipoproteins‚ and phospholipids. The lipopolysaccharide is on top of the
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bath to flatten the sections. The sections were picked up onto the microscope slide‚ drained it in vertical position for several minutes. The slides were then placed on a slide holder in the oven at 37 °C overnight. Step 5: Staining The slides were placed in a staining rack and were dipped into xylene for 5 minutes and repeated once in new xylene for another 5 minutes. Then‚ the slides were dipped into
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NTRODUCTION Often in the study of microbiology‚ the need to identify an unknown microbe may arise. This aids in the recognition of which microbes are harmful or pathogenic as well as those that may be beneficial to us in some way. This report will detail how a similar project was performed in order to identify two unknown micobes using the techniques that were learnt during the course of the semester. We embarked on the project with the knowledge that we would have to identify a Gram(+) as well
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8.1 MICROWAVING Principle: Microwaving is a method used for heating of tissue sections and cellular components with the use of a microwave which provides heat by electromagnetic waves that has a high frequency‚ with a wavelength if one millimetre to one meter and an intermediate between infrared and shortwave radio wavelengths (Miffin‚ 2002). This allows fast fixation. Technique: Electromagnetic waves are commonly used for carrying out energy and are said to be powerful for non-invasive processing
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MICR 300A GENERAL MICROBIOLOGY 1 (4)‚ Fall 2014 Lecture (01) M/W 9:50 am – 11:05 pm‚ SH C244 Lab (02) M 11:15 am – 1:45 pm; Lab (03) W 11:15 am – 1:45 pm; ASCL 226 Instructor (Lecture): Dr. Moon H. Lee Office Hours: Mon/Wed 8:45 am-9:45 am; 11:15 am – 12:15 pm Office: BIOS 262‚ Tel: (323) 343-2064‚ Email: mlee@LABioMed.org Instructor (Lab): Dr. Hao Howard Xu Office Hours: Mon‚ 2:40 pm – 4 pm Office: Tel: (323) 343-2188‚ Email: hxu3@calstatela.edu
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Observation of Bacteria on Chicken and Handrail and the Effect of GermX® and Alcohol on Them. Sujit Bhimireddy‚ Daryl Onwuchuruba‚ Naveen Sood‚ and Manasa Tripuraneni Biology 106 Abstract Bacteria are found everywhere on this planet. This experiment is aimed at observing some of the bacteria that surround us in places that we use everyday. We will look at bacteria found on a piece of chicken and bacteria found on a handrail. The effectiveness of antibacterial substances such as GermX® and
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Title: Cell Structure І Objectives: 1. Prepare specimens for staining. 2. Identify unique and standard structures of the microscopic world. 3. Identify and use different stains for the different types of organelles. Results: a) Epidermal cells of plants: Upper surface or Rheo discolours leaf Low power: 40X Upper surface or Rheo discolours leaf Low power: 40X Upper surface or Rheo discolours leaf High power: 100X Upper surface or Rheo discolours
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