if the unknown organism is a Gram positive or Gram negative. This is the initial step that must be taken before any other lab procedure may continue on to ensure the purity is present‚ the arrangement of the cells‚ and the shape the cell has. The staining of the cell starts off with using the primary stain‚ Crystal Violet which is a purple color‚ to begin with. Next‚ Gram Iodine is added on‚ which is our mordant and this causes a complexion with the primary stain‚ results in formation of insoluble
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stored at room temperature (approximately 25°C). Use and Lab Safety: The materials supplied are for use with the method described in this kit only. Use of this kit presumes and requires prior knowledge of basic methods of gel electrophoresis and staining of DNA. Individuals should use this kit only in accordance with prudent laboratory safety precautions and under the supervision of a person familiar with such precautions. Use of this kit by unsupervised or improperly supervised individuals could
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Restriction Endonuclease Digestion of DNA from E. coli cells and Analysis by Agarose Gel Electrophoresis Introduction The main goals of this experiment are testing an alternative procedure called “boiling lysis”‚ evaluating the quality of the purified plasmid for restriction digests‚ and identifying the mislabeled plasmid. The plasmid DNA from a carrier E. coli strain was purified by the boiling lysis. In the boiling lysis method‚ the bacterial cells were given momentary heat treatment
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The prokaryotes Student name : Lina Qasem AL-hyasat . Engineer : Ahmad Shrab . Introduction This lab is purposed to familiarize basic equipment and techniques used in the study of microorganisms. In addition‚ learn some basic techniques used in identifying prokaryotes and make and view microscope slides of some common prokaryotes. In this lab ‚ I worked two experiments ‚ the first
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Rocky Mountain Spotted Fever Rickettsia rickettsia was named after the first person who discover Rocky Mountain Spotted fever‚ Howard Taylor Ricketts. The unicellular aerobic gram negative non-motile‚ non-spore forming‚ coccobacilli bacterium are 0.3-0.5 x 0.8-2.0 um. It is in the family Rickettsiaceae and order Rickettsiales of the α-Proteobacteria. It is a unicellular microorganism that lacks a distinct nucleus and membrane- bound organelles‚ while it is a three-layered cell wall and its outer
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Gel electrophoresis is a routine laboratory procedure in biochemical studies that takes advantage of a protein’s amphoteric nature to determine its molecular weight and charge by running the sample through a gel matrix under the influence of an electrically charged field. A popular example of gel electrophoresis is Sodium dodecyl sulfate-polyacrylamide electrophoresis or SDS- PAGE which has been used in this experiment to supposedly determine albumin and casein’s molecular weights respectively. The
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Classification of Epithelia Lines Examples Simple squamous Surfaces involved in passive transport Alveoli Endothelium Loops of Henle Simple cuboidal epithelia Surfaces involved in secretion and absorption Some glands Ducts Collecting tubule of kidney Simple columnar epithelia Surfaces involved in high rates of secretion and absorption Protection Stomach and small intestine where it has microvilli Collecting tubule of kidney Fallopian tubes where it is ciliated Pseudostratified Usually ciliated
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classification of the invisible bacterial life. Bacterial classification is based on several major properties‚ CLASSIFICATION OF BACTERIA BASED ON GRAM STAINING This method of classification of bacteria was discovered by Hans Joachim Christian Gram 1883‚ a Danish Physician‚ while attempting to differentiate bacteria from human tissue by different staining methods. He noted differences in stain retention by bacteria due to
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Name Microbiology 2210 Answer Key Practice Test 1 Directions: Choose the most correct response to answer the statement. ___1. Archaea a. belong to the kingdom Protista b. have petidoglycan in their cell walls c. belong to the domain Bacteria
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DMLS/FT/1B/02 Admin number: P1138687 Summary This experiment aims to observe mitosis and meiosis on cell specimens. It involves the modelling of the stages of meiosis and also the staining procedures to identify mitosis in onion root tip. There are two parts to the procedure of the experiment whereby the first is staining and observing mitosis in onion root tips and the second being observing and comparing mitosis between whitefish blastula and onion root tip. The results are that the five stages of
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