Hartpury College Fdsc Equine Science Animal Microbiology Diagnosis and treatment of disease UIN XGJ-10-1 Amanda Finch Date Set: 20th September 2010 Date due: 17th November 2010 Contents page Page Title page i List of contents
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Screening and identification of rhizobacteria isolated from heavy metal contaminated soils INTRODUCTION As a result of human activities such as mining‚ agriculture and industrial activity‚ heavy metal pollution has become one of the most serious environmental problems today. Elevated levels of heavy metals not only decrease soil microbial activity and crop production‚ but also threaten human health through the food chain. Soil microorganisms can degrade organic contaminants‚ while metals need
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cause eye infections in men and women which can lead to blindness. It can also be passed from mom to baby during birth in the form of eye infections and pneumonia. HOW WAS IT DISCOVERED? c. Trachamotis was first discovered in 1907 by Giemsa staining by Halberstaedter and von Prowazek who took scrapings from individuals infected with trachoma and inoculated orangutans eyes with the samples. This demonstrated how the disease could be spread between infected individuals. In 1929‚ there was an
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1. EGG INOCULATION The fertile hen’s egg can be used to cultivate and propagate various types of viruses. Because of the ability to alter their tropism and to adapt to a new host species‚ many viruses become capable of growing in chick embryo tissues wherein they frequently attain a much higher concentration than in the tissues of the natural host. STRUCTURE OF AN EGG The extra-embryonic membranes of the chick embryo arise from three germinal layers: the endoderm‚ mesoderm and ectoderm
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Identification and characterization of Pierisin gene in P. napi The strategy was followed to amplify the Pierisin-6 gene was given in the supplementary Fig. 1. The mRNA of pierisin-6 gene was purified from the total RNA of fifth instar larvae of P. napi‚ the conserved region was amplified using gene specific primers (supplementary table-1)‚ cloned into pGEM®-T vector and sequenced. Further‚ 3’ and 5’ untranslated regions of pierisin-6 gene were identified by 3’/5’ RACE-PCR. The amplified cDNA sequence
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1. By using two specific examples of an infectious disease and cancer (lymphoma or leukemia‚ etc) describe the application of the following techniques in detection and diagnosis. a) Flow cytometry analysis “Feline Panleukopenia (FP) is a highly contagious viral disease of cats caused by the Feline Parvovirus‚” (American Veterinary Medical Association‚ 2013). This disease is a secondary infection that caused by the apoptosis of infected animal’s cells and reduces the expression of interleukin-2
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1.Summarize the formation of friction ridge skin and how it relates to the permanence of fingerprints. The skin over most of our bodies is fairly smooth. ’Friction Ridges ’‚ however‚ are found on the digits‚ palms and soles. They are called ’friction ’ ridges because of their biological function that helps us grasp and hold onto objects. Sometimes compared to fine lines found in corduroy‚ unlike corduroy‚ ridges vary in length and width‚ branch off‚ end suddenly and‚ for the most part‚ flow with
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proteins can be detected with in cells by a technique known as Cytochemistry. Cytochemistry is the practice of fixing‚ staining‚ and examining cells (Lab Manual‚ Chp. 5). Cytochemistry is an in situ experiment‚ meaning that any work performed is done on a normal‚ intact cell. The practice of fixing is important because it prevents the loss of components from their locations‚ likewise staining is equally important because it helps us to easily identify components in a cell by increasing the contrast between
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Question and Answer 1. Paper reported that Olfm4 deletion induces colon adenocarcinoma in APCmin mice‚ so why after miR-34 deletion‚ olfm4 positive stem cell number was increased and tumor incidence was also increased? Their research was working on the colon tumorigenesis‚ but we are working on the small intestine. Also in our model‚ colon tumor is very rare‚ could because of different genetic background. The function of Olfm4 may be also tissue specific. 2. Do these changes caused by the genetic
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Tobacco -a preparation of the nicotine-rich leaves of an American plant‚ which are cured by a process of drying and fermentation for smoking or chewing. Shorts of smoking cigarettes: Irritation and inflammation of the stomach and intestines Increased risk of painful ulcers along the digestive tract Reduced ability to smell and taste Premature wrinkling of the skin Higher risk of blindness Gum disease (periodontitis) Long effects of smoking cigarettes : 1. Smelling like smoke There’s no mistaking
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