be a perfect ideal antibiotic. In this experiment six antibiotics will be tested to determine which is the most effective on gram-negative bacterium Escherichia Coli and gram-positive bacterium Bacillus Cereus. In previous class experiments‚ gram-staining was conducted to determine the qualities of gram-positive
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Hong Kong Diploma of Secondary Education Examination Biology and Combined Science (Biology part) School-based Assessment Sample Tasks Teachers may use the sample tasks for non-profit making educational and research purposes with proper acknowledgement. ©香港考試及評核局 保留版權 2009 Hong Kong Examinations and Assessment Authority All Rights Reserved 2009 HKDSE Biology SBA Practical Task: Topic: I (e) Presence of protease in pineapple and / or kiwifruit Scenario: Last week‚ Mary prepared some jelly
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BAsic ReseARch PAPeR BAsic ReseARch PAPeR Autophagy 9:5‚ 1–16; May 2013; © 2013 Landes Bioscience Inhibition of the autophagic flux by salinomycin in breast cancer stem-like/progenitor cells interferes with their maintenance Wen Yue‚1‚2‚† Ahmed hamaï‚1‚2‚† Giovanni Tonelli‚1‚2 chantal Bauvy‚1‚2 Valérie Nicolas‚2‚3 hugo Tharinger‚2‚3 Patrice codogno1‚2 and Maryam Mehrpour1‚2‚* 1 † These authors contributed equally to this work. Keywords: breast cancer stem-like/progenitor cell
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BIO304: Forensic DNA Fingerprinting Lab DNA Fingerprinting‚ method of identification that compares fragments of DNA. It is sometimes called DNA typing. With the exception of identical twins‚ the complete DNA of each individual is unique. A DNA fingerprint is constructed by first extracting a DNA sample from body tissue or fluid such as hair‚ blood‚ or saliva. The sample is then segmented using enzymes‚ and the segments are arranged by size using a process called electrophoresis. The segments are
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pylori were assessed by gram staining to be assuring as a spiral form. Then‚ helical bacteria were suspended in Brucella broth (Merck‚ Germany) supplemented with 10% fetal calf serum and 2% L-cystein‚ and incubated overnight at 37°C in a microaerophilic rotary shaker (100 rpm). When
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The introduction of the microscope as a tool for the biologist brought about a complete reappraisal of the micro- composition of biological tissues‚ organisms and cells. In the infancy of its application to organic materials‚ it was the implement of anatomists and histologists in particular‚ where previously unimagined structures in cells were revealed. More recent developments in biological specimen preparation have come from biochemists and physicists who have used the microscope to examine cells
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Objectives Protein Isolation: Protein isolation for a western blot uses detergents and mechanical force to separate seeded cells from their container. Eukaryotic cells are attached to the surface of a flask by cadherins. In the past‚ we’ve separated the cells from the flask by breaking these bonds with a protease‚ but in order to keep the proteins intact‚ a different method needs to be used to extract the proteins. In protein extraction for a western blot‚ we use detergents to lyse the membrane
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Bulimia nervosa‚ also known as bulimia‚ is an eating disorder that affect people all over the world. Bulimia id characterized by binge eating followed by purging. Binge mean to eat a large amount of food in a short amount of time. Purging mean to try to get rid of the large amount of food consume. It’s believe that bulimia is associated with other mental disorder such as depression‚ anxiety‚ and problems with drugs and alcohol. It’s estimated that 6.5 million people suffer from bulimia globally.
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loss of potassium‚ sodium and chloride from the body as a result of purging behaviours. • Potential for gastric rupture during periods of bingeing. • Inflammation and possible rupture of the oesophagus from frequent vomiting. • Tooth decay and staining from stomach acids released during frequent vomiting. • Chronic irregular bowel movements and constipation as a result of laxative abuse. • Peptic ulcers and pancreatitis Health Consequences of Anorexia Nervosa In anorexia nervosa’s cycle of
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tissues were fixed in neutral buffered formalin and embedded in paraffin‚ sectioned at 5 μm and subsequently stained with hematoxylin/eosin to see the architecture of hepatic tissue and inflammatory cell infiltration. Sirius red staining for fibrosis and Prussian blue staining for iron deposition were also done in liver sections. Sections were then studied and photographed under light microscope (Zeiss Axioscope) at 40 magnifications. Collagen deposition was semi quantitatively measured using NIH Image
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