EXPERIMENT NO. 15 PROTEIN CHARACTERIZATION BY ELECTROPHORESIS Abstract The molecular weights of protein extracts were assessed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Two sets of four protein samples‚ standard bovine serum albumin (BSA)‚ invertase‚ egg albumin‚ and casein‚ were prepared; one set containing β-mercaptoethanol (BME) while the other did not. These were then analyzed through SDS-PAGE with 12.5% resolving gel‚ prepared using 2 M Tris-HCl at pH 8
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ABSTRACT The title of our project was Bilimbi fruit as an alternative stain remover. The purpose of this experiment was to find out if Bilimbi can remove stains from our clothes. We all know that teenagers today are very eligible especially in wearing dress. We conduct this kind of study to be able to have another background study about natural bleaching agent and to build natural product that is a solution for a stains The procedure involved was to apply the extract of Bilimbi to the clothes
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significantly after which they can be seen under the light microscope as distinct thread-like structures. Chromosomes are usually studied by obtaining actively dividing tissue‚ fixing the material to kill the cells‚ and to preserve their structure‚ staining the chromosomes with a dye‚ and then observing them with a microscope. By studying mitosis in this way‚ changes that occur in the chromosomes can be observed and the visible events that bring about the process of heredity in somatic (body) cells
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3.3 Microbiological Analysis 3.3.1 Bacteria count using Heamocytometer This method was used to determine the of three types of dessert but does not differentiate the type of bacteria. 25 g of food samples will be homoginesed in a sterile stomacher bag and shaken for two minutes with 225 ml of peptone water to obtain the food mixture. Using separate sterile pipets‚ decimal dilutions of 10-2‚ 10-3‚ 10-4‚ 10-5 will be prepared and others as appropriate‚ of food homogenate by transferring 10 ml of previous
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conversation with miss Qutachi‚ I found that she has not made new friends in the city. On exam‚ I found a few cavities in her molars and generalized staining on her teeth. I asked her if she has any idea on why she developed stains on teeth. I allowed her to led the discussion on stains and she pointed out to coffee and smoking habit as potential reasons for the staining. Once she started talking about smoking‚ I asked her permission to collect some information on smoking history. I learned that she has been
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Compound Microscope Parts A high power or compound microscope achieves higher levels of magnification than a stereo or low power microscope. It is used to view smaller specimens such as cell structures which cannot be seen at lower levels of magnification. Essentially‚ a compound microscope consists of structural and optical components. However‚ within these two basic systems‚ there are some essential components that every microscopist should know and understand. These key microscope parts are illustrated
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Microbiology! 1) Groups of Organisms (7): Eukaryote: TRUE nucleus 1. Fungi 4. Bacteria 2. Algae 5. Rickettsia 3. Protozoa ! Prokaryote: NO Nucleus Ribosome is the only Organelle 6. Mycoplasma (7. Viruses: No cell structure‚ ARE NOT CELLS) Microorganisms are: ! UBIQUITOUS! ! HARMLESS IN THEIR NORMAL HABITAT ! OPPORTUNISTIC (If they leave their normal habitat and get into us) 2) Roles of Microorganisms: (Bacteria and Fungi are “Semi-Good”) - digestion -
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Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright © 2001 by Wiley-Liss‚ Inc. ISBNs: 0-471-37972-7 (Paper); 0-471-22390-5 (Electronic) 12 Electrophoresis Martha L. Booz Chemical Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . What Is the Safest Approach to Working with Acrylamide? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . What Are the Symptoms of Acrylamide Poisoning? . . . . . . What
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Discussion Aloe Vera is greatly useful in the treatment of gingivitis or periodontitis (17). It decreases inflammation‚ bleeding‚ and edema of the gingiva. It is a strong antibacterial in periodontal pockets where normal cleansing is challenging‚ and its antifungal properties help considerably in the difficulty of denture stomatitis (18) .also Aloe Vera is a possible anti-bacterial factor which is mentioned to be useful in fighting the microorganism and controlling periodontal disease (19). Vijayalakshmi
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Brunet Ms Owen October 14th‚ 2012 Part 1 Purpose To culture and observe the various types of bacteria found around Canterbury High School. Materials and Methods Refer to pages 422-425 of Biology 11 McGraw-Hill Ryerson and the handout “Gram Staining Procedure”. Observations Table 1: Locations of Bacteria Samples Quadrant | Location of Sample Obtained | 1 | Floor | 2 | Water fountain head | 3 | Auditorium Chair | 4 | Inside of Boys’ Bathroom Door Handle | Table 2: Growth of Bacteria
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