Review all chapters in your exercise manual beginning with the introduction INTRODUCTION: (Covered 9.4.14 II Week 1) Biosafety levels1: basic level of containment. Hand washing or wearing gloves 2: Appropriate for working with human body fluids. Autoclave‚ sharps containers‚ lab coats 3: appropriate for working with pathogens that can be transmitted via respiratory route. Self-closing‚ double doors and sealed windows 4: Highest level. Aerosol pathogens; pathogens with no vaccine/treatment. Separate
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PSBPP103 Differential Gram’s staining EXPERIMENT NO. 1 AIM: THEORY: Page No: C1 Date: To Gram stain the given bacterial suspension and to differentiate between gram positive and gram negative organism. Visualization of microorganisms in the living state is very difficult‚ not just because they are minute‚ but because they are transparent and almost colorless when suspended in an aqueous medium. To study their properties and divide microorganisms into specific groups for diagnostic purposes
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prokaryotes are and The two (2) genera of bacteria that are capable of producing spores are and b) c) d) An example of a Gram positive bacteria is example of a Gram negative bacteria. and is an e) Three (3) common differential staining methods used in microbiology are ‚ and "Ubiquitous" in microbiology means The minimum inhibitory concentration (MIC) means Of more than three thousand (3000) species of bacteria‚ only 10% cause disease. The three (3) diseases and their causative
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Through processes know as wet mounts and simple stains‚ observing living microorganisms through a compound microscope can generate a clearer understanding of their appearances and movements. Microorganisms are living things that for the most part cannot be seen through the naked eye. They live on and among humans‚ as well as plants‚ animals‚ and all that is a part of the Earth. Harmless as most seem to be to humans‚ microorganisms are an important relevance of study for human interest in varied perplexities
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Week 1 Chapter 3 Staining Lab Quiz SCI/250 SCI250 Week 1 Chapter 3 Staining Lab Quiz (6.7 points each) Check or underline the correct answer Section: Multiple Choice 1. Which of the following stains is used frequently to identify Mycobacterium and other bacteria whose cell walls contain high amounts of lipids? Gram stain Schaeffer-Fulton stain Acid-fast stain Lipid stain Spore Stain 2. Which of the following stains is used to classify microorganisms based on their cell wall
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Study guide for Microbiology Chapter 2 Name________________ 1. List and describe the sequence steps routinely used to identify bacteria. These are referred to as the “five I’s” in your text. 2. Define / describe each of the following as they apply to microbiology: a. Culture f. pure culture b. Inoculum g. contaminated culture c. Inoculation h. mixed culture d. Colony 3. Microbiologists employee a number of approached to acquiring a pure culture
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application of simple stains or dyes and hence appear as nonstaining entities in Gram-stain preparations. However‚ once stained‚ endospores are quite resistant to decolorization. This is the basis of several spore stains such as the Schaeffer-Fulton staining method which also differentiates the spores from sporangia and vegetative cells. However this method is not suitable for taxanomic purposes.
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tuberculosis distinguishable from nearly all other bacteria. Acid-fast staining is‚ therefore‚ known as a differential stain. Mycobacterium and some Nocardia species are considered acid fast because‚ during the acid-fast procedure‚ they are able to retain the primary dye even when decolorized by a powerful solvent known as acid alcohol. Most other bacterial genera are easily decolorized by acid alcohol. Ziehl/Neelsen Acid-Fast Staining Procedure In later years‚ Ehrlich’s technique was improved upon
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harvard.edu/node/222 BSP‚ S. (2010). How is EM different from light microscopy? Retrieved April 25‚ 2015‚ from http://bsp.med.harvard.edu/node/222 Tortora‚ G.‚ Funke‚ B.‚ & Case‚ C. (2010). Staining Techniques. Retrieved April 25‚ 2015‚ from http://www.cliffsnotes.com/sciences/biology/microbiology/microscopy/staining-techniques
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Purpose/Question being addressed: The purpose of this experiment is to determine which kind of drinks stain your teeth the most. The drinks that will be tested are tea‚ coke‚ and coffee. B. Research Facts/ Hypothesis: Research shows that the darker the drink is that you drink the yellower your teeth will become. Teeth are naturally white or off-white in color but build- up from foods and drinks such as coffee and tea over
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