Effectiveness of Basella rubra (Alugbati) Fruit Extract as a Counterstain in Gram Staining Investigators: Astilla‚ Yasmin Marie Cala‚ Maria Victoria Bianca Ko‚ Mariell Lizardo‚ Francis Dominic Mariano‚ Maria Cristina Querol‚ Maria Zennah Quinto‚ Camille Rosales‚ Ana Mariella Valmonte‚ Kyla Nicole Villalon‚ Gian Angelo 4MT-2 Statement of the Problem Can Basella rubra fruit extract be used as a counterstain in Gram staining? Introduction & Background According to H. J. Croxx‚ when microscopists
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BTN 211 TOTORIAL 1 Chapter 1 1. The History and Scope of Microbiology Which of the following distinguish the field of microbiology from other fields of biology? a. the size of the organism studied b. the techniques used to study organisms regardless of their size c. both the size of the organism studied and the techniques employed in the study of organisms d. neither the size of the organism studied nor the techniques employed in the study of organisms regardless of their size Who of
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BIOSC 0050 Oct. 9‚ 2012 Dead or Alive? (Week 2) Post-Lab Assignment Results: The results of the Feulgen’s Schiff’s Stain test are that samples ALV-188-23 and ALV-843-20 turned magenta and samples ALV-882-21 and ALV-694-02 did not turn magenta. The result of the tetrazolium test is that all four of the samples did not turn pink. We were not able to conduct the detailed analysis of cultured plates with growth‚ so not results are available for this test. Discussion: For the Feulgen’s/Schiff’s
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Introduction By examining blood smear by microscopy it can detect infections whether they are viral or bacterial. Blood smears are examined by looking at the white and red blood cells appearances. The aim of the experiment is to compare a normal blood smear and its pathological features to those with an infection or abnormality by the use of microscopy looking at the cells involved with blood. A normal blood smear would contain 3 to 4 white blood cells per field in microscopy. If there is an
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In biology 102 lab‚ I conducted an experiment dealing with worms and how they regenerate or “grow”. We did this study over a specific type of worm called Planaria‚ which are simple‚ small‚ flat worms that reside in aquatic environments and are free-living (Brewton‚ 2017‚ p. 44). To start‚ I went over to the lab counters and obtained my worm with a plastic pipette‚ sucking up the worm with a little bit of water and placing it in a tiny covered plate. The water was essential to include with the worm
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Del Mundo‚ Layah Basella rubra as a biological stain Experimental The alugbati extract turned out to be favorable stain for plant nuclei and organelles especially for Hydrilla verticillata and as substitute for crystal violet or safranin in Gram staining. The extract stained the bacteria red-orange and the plant nuclei‚ and the cytoplasm red and light pink‚ respectively. The study is limited to the ability of Basella rubra (alugbati) to be used as a biological stain 2011 Gel-O-Fury- Saavedra‚ Ralph
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are acidic dyes since they do not cross the cell wall/membrane particularly easily. A background stain is a stain used to stain everything other than the bacteria. The Gram stain is one of the oldest‚ most cost-efficient‚ yet most under-utilized‚ staining method used to identify bacteria. It consists of a basic dye (the primary stain crystal violet)‚ a mordant (Gram ’s iodine)‚ decolorizer (acetone:alcohol) and a counter stain (safranin). A mordant is a compound which helps hold the primary stain
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Microbiology UNKNOWN LAB REPORT Kateryna Petrakova INTRODUCTION Microorganisms cause a great amount of diseases. For healthcare providers it is very important to be aware of what organisms are pathogenic and cause a disease and‚ therefore‚ to find an appropriate treatment. Different microorganisms require various environments in order to replicate and to become dangerous for a person’s health. An integral part of any medical treatment is to be able to recognize and
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experiment‚ gram staining skills develop. More understanding the types and morphology of bacteria. Expected experimental result‚ Escherichia coli (E.coli ) is a negative gram bacteria which stain pink colour ‚ while Staphylococcus aureus ( S.aureus ) is a positive gram bacteria which stain purple colour. Materials Bacteria : Escherichia coli ‚ Staphylococcus aureus Crystal Violet Gram’s iodine Absolute alcohol Safranin Methodology As per manual. Discussion Gram staining is a very importance
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First‚ the matting was placed on the four slides‚ cover-slips as well as the container of blood on top. Blood containers are in dropper bottles. Number each slide with a wax pencil. A drop of blood was placed on a slide and numbered it slide #1. Slide #1: 1. With the wooden applicator stick‚ dip the end into the blood and place a tiny drop onto slide #1 and put cover slip over it. Slide#2 1. Place 1 drop of 0.9% NaCl with the drop of blood (use the filter paper to absorb excess liquid). 2.
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