University of Texas at Tyler Lab 3C: Purification of L-Lactate Dehydrogenase By Affinity Chromatography on Cibacron-Blue Sepharose David Alexander 10-15-2014 Dr. Black Chem 4135.001 Abstract: Like the previous experiments‚ the ultimate goal of this lab was to purify the enzyme sample. However‚ this is the last lab for purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally
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that iron serves as the catalyst in the light-producing chemical reaction. The Luminol first reacts with the hydroxide molecules to form two negative charges‚ or dianion. The dianion then reacts with oxygen to form a 5-aminophtalic acid and nitrogen gas. After‚ the oxygen forms with the hydrogen peroxide and the
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> # Ho’s Maple Lab Test Solution: Semester 1 2012 (1) # Question 1; > evalf(100*sin(95)‚38); 68.326171473612098369957981656827095404 > # Queston 2; > f:=x->3*sin(1/4*x^4)-sin(3/4*x)^4; (2) > # Find 1st derivative; > D(f); (3) > # Find turning/stationary point in the interval [1‚2]‚ 1st derivative expression = 0‚ 10 significant figures!; > evalf(fsolve(3*cos((1/4)*x^4)*x^3-3*sin((3/4)*x)^3*cos((3/4)*x)= 0‚x=1..2)‚10); 1.562756908 (4) > # Find 2nd derivative at x= 1.562756908; 10 significant
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IB Chemistry Experiment 4 Composition of a Post-1983 Penny Lab Report Background: Through the use of percent composition one can easily find out how much of a certain element is in a compound or an item such as a post-1983 penny. The experiment will be using HCl a strong acid to dissolve all the zinc inside the outer copper shell. The reaction follows as such; Zn+2HCLZnCl2+H2‚ but there will be no reaction with copper. The portion that will be measured is the mass of copper left after all of
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b.) The mass decreased or increased in each cylinder‚ depending on if the solution was hypotonic‚ hypertonic or isotonic. In Cylinder (A) the mass decreased‚ so liquid was released from the potato‚ because the cells in the potato would have burst if even more was absorbed‚ whereas the length stayed the same. In Cylinder (B) the mass and length increased‚ which means the potato cells absorbed some Sodium Chloride (NaCl)‚ so the cells don’t shrivel up and die. For Cylinder 3 the mass and length also
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Osmosis Lab Report Hypothesis: Osmosis will occur when there is an uneven distribution of solute in a solvent. The higher the solute in solvent‚ then there will be a higher rate of osmosis through the diffusion gradient forming a hypertonic or hypotonic solution. Solvent with equal or no solute forms an isotonic solution. Materials: Distilled water‚ sucrose‚ dialysis tubing‚ string‚ 250 ml beaker. Procedure: To demonstrate and isotonic solution we needed 3 inches of dialysis tubing. We
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eLearning 2009 Publication No. 91860 Rate of Reaction of Sodium Thiosulfate and Hydrochloric Acid Rate Laws Introduction The purpose of this demonstration is to investigate the effect of sodium thiosulfate concentration on the rate of reaction of sodium thiosulfate with hydrochloric acid. The reaction‚ which produces solid sulfur‚ will be followed by measuring the time needed for the reaction mixture to become opaque. The results will be analyzed graphically to determine the order of
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SCIE211 Phase 2 Lab Report Title: Speciation Instructions: You will need to write a 1-page lab report using the scientific method to answer the following question: • What would happen if a species within a population were suddenly split into 2 groups by an earthquake that creates a physical barrier like a canyon? When your lab report is complete‚ post it in Submitted Assignment files. Part I: Use the animated time progression of speciation to help you write up your lab report. Part II: Write
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Separating Substances: Identifying Food Dyes with TLC Background The color of food is an integral part of our culture and enjoyment of life. Who would deny the mouth-watering appeal of a deep-pink strawberry ice cream on a hot summer’s day or a golden Thanksgiving turkey garnished with fresh green parsley? Even early civilizations such as the Romans recognized that people "eat with their eyes" as well as their palates. Saffron and other spices were often used to provide a rich yellow color
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December 8‚ 2013 Soil Analysis Lab Report Intro: Soil is produced over hundreds of years through the weathering of rocks and the decomposition of organic material. Soil contains nutrients that are taken from decomposed animals and plants which turn to detritus. Nutrients such as nitrogen are added to the soil in these ways by organisms such as nitrogen fixing bacteria. Soil is important because it is the fuel to the plants that we need in order to survive and for all species to survive. Soil
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