Introduction In this experiment‚ the growth of four cultures‚ which are Escherichia coli‚ Staphylococcus aureus‚ Saccharomyces cerevisiae (yeast) and Penicillum (mold) were examined under a range of acidic to alkaline environments to determine the pH requirements of the four species. By conducting this methodology‚ the range of acceptable pH limits‚ including the optimum pH for each organism‚ can be determined. The range of pH over which an organism grows is defined by three cardinal points: the minimum
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resembling grapes that is was a gram positive cocci. A Catalase test was then performed using hydrogen peroxide. A positive catalase test was observed when bubbles started to form on the culture distinguishing Staphylococcus spp. form on the culture distinguishing Staphylococcus spp. from Streptococcus spp. Finally‚ a Mannitol Salt Agar was streaked and incubated at 37 degrees Celsius for 24
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COMPARISON BETWEEN PLACENTA AGAR AND NUTRIENT AGAR ON GROWTH OF Staphylococcus aureus AND Escherichia coli Chloe Dominique Acero Kristine Marie Gonzales Hannah Marie Hermosisima Patrisha joy Morales Joanna Keziah Ramos Group 4 BSMT3E Background of the Study Placenta is an organ characteristic of a true mammal during pregnancy‚ joining mother and offspring‚ providing endocrine secretion and selective exchange of soluble‚ but not particulate
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plate seemed to show that E. coli did not have lipases because it did not have clear zones. Staphylococcus aureus seemed to have these clear zones meaning it can secrete lipases. The bacteria Bacillus subtilis did not grow well to see if there was a clearing. After doing some research‚ it was shown that B. subtilis is lipase positive so there should have also been some clear sones like the Staphylococcus aureus (Watson). The DNAse agar could not have been analyzed because HCl was not available to
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------------------------------------------------- ------------------------------------------------- EVALUATION OF EFFECTIVITY OF SOAPS AND DETEGENTS AGAINST BACTERIA BACTARIUM EVALUATION
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bacteria. Enteric. 2. The outer layer of the skin is the _________ while the deeper layer is the _________ cutaneous‚ keratin. dermis‚ sebum. mucous‚ epidermis. epidermis‚ dermis. sebum‚ keratin. 3. A massive lesion caused by the spread of Staphylococcus aureus infection (often on the neck and upper back) is called a ________ boil. furuncle. Carbuncle. abscess. pustule. 4. Scalded skin syndrome is caused by exotoxins (exfoliatins)
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2. Material and methods 2.1 Sample collection The following 14 samples of Staphylococcus aureus were used in this study. All samples were obtained from food contact surfaces at three food premises in University of Putra Malaysia (UPM). Samples were collected from the surface of each material according to standard swabbing techniques. The collection was carried out during weekdays (15:00-17:00) after finished the preparation of foods. Sterile swabs were removed from the coded test tubes that contain
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blue onto the smear for 30 second‚ rinsed with water‚ blotted dry and observed the slide using oil immersion. Gram Stain For comparison purpose‚ I used two bacteria for this experiment. They are E. coli‚ a gram negative bacteria and Staphylococcus‚ a gram positive bacteria. I prepared a heat fixed smear of both bacteria. First‚ I used crystal violet as my primary stain‚ put few drops of it on the smear and let it sit for 30 seconds.
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Horack’s article “Staphylococcus epidermidis”‚ S. epidermidis is described as “gram-positive cocci bacteria that are part of the normal flora on the skin and nasal passages.” The article goes on to say that the species was originally named Staphylococcus Albus by microbiologist Rosenback in 1884. When viewed under a microscope S. epidermidis will appear in chains‚ pairs‚ or grape-like clusters (Horak 1). Taxonomically‚ the species S. epidermidis falls in the genus Staphylococcus‚ which is in the
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Catalase Test showed catalase positive results. This is a key characteristic of the Staphylococcus species (Bergey). Ex 2-2 Colony Morphology showed circular white opaque colonies with smooth‚ entire margins when grown on TSA. This is key in helping to determine whether the EI was a Staphylococcus spp or a Mircroccus spp since Mircroccus spp. commonly show yellow color colonies when grown on TSA and Staphylococcus spp are commonly opaque white colonies when grown on TSA (Bergey). Additionally‚ Ex
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