Virtual Lab: Classifying Using Biotechnology Worksheet NOTE: As you read the information in the Microbiology Handbook‚ there may be some terms you are not familiar with – such as 16s ribosomal RNA and Polymerase Chain Reaction. Refer to your text to read background material explaining any terms or processes with which you are not familiar. Record the results of your investigations of each unknown in Table 1 by completing the following steps: 1) Apply the stain to your first
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procedure 29‚ “pH and Microbial Growth” in Benson’s Microbiological Applications: Laboratory Manual in General Biology‚ 12th edition. My group was assigned the microorganism Staphylococcus simulans. Conclusion: The plotted data defines Staphylococcus simulans’ optimum pH level to be 8.0. Based on this‚ I conclude that Staphylococcus simulans is a neutrophile growing best near a neutral pH level. From the results of groups EC-1 and EC-2‚ Escherichia Coli is a alkaliphile. However‚ based on the fact
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to identify the anti-bacterial property of Ashitaba non-polar extract against gram positive organism‚ Staphylococcus aureus. There were six disk namely the different concentrations of Ashitaba extract (12.5 %‚ 25%‚ 50%‚ 100%)‚ the positive control ( Penicillin) and the negative control ( distilled water). Table 4.1 Results of anti- bacterial property of Ashitaba extracts against Staphylococcus aureus sample Zone of inhibition (mm) 12.5 % 25 % 50 % 100 % Distilled water Penicillin
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Methicillin-resistant staphylococcus aureus (MRSA)‚ is a gram positive bacterial that is normally found on the skin and in the throat‚ and is a life threatening staphylococcal infection that may arise within hospitals‚ and “is frequently responsible for abscesses‚ endocarditis‚ impetigo‚ osteomyelitis‚ pneumonia‚ and septicemia.” Treatment usually includes bed rest‚ analgesics‚ and an anti-microbial drug that is resistant to penicillinase‚ an enzyme secreted by many species of staphylococcus. This writer
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to endotoxin-induced shock? Staphylococcus aureus Pseudomonas aeruginosa Klebsiella Proteus Serratia 3. Rheumatic fever caused by Streptococcus pyogenes most likely results from ________ the invasion of heart cells by bacteria. the growth of bacteria on heart cells without invasion. a reaction between heart antigens and antibodies against bacteria. viral infections of the heart muscle. a toxin produced by bacteria. 4. What causes septic shock? Staphylococcus aureus Any organism growing
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Abstract The primary focus of this lab was on microscopy and simple stains. Crystal violet and Carbol fuchsine‚ simple staining components‚ were used to stain the slide in order to see the different microbes in order to determine their cellular shape and identify unknown ones by comparing. Introduction Bacterial cells are usually colorless because cytoplasm‚ for the most part‚ is transparent. Since the bacteria are colorless‚ it is almost essential to add a stain to make the bacteria more visible
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balsamifera) Leaves‚ Onion (Allium cepa) Bulb‚ and Takip Kuhol (Centella asiatica) Leaves Extract as Antibacterial of Salmonella typhimurium‚ Staphylococcus aureus and Pseudomonas aeruginosa II. Table of Contents I. Title Page II. Table of Contents III. Abstract III. Abstract: Diseases and wound infections cause by the bacteria Salmonella typhimurium‚ Staphylococcus aureus and Pseudomonas aeruginosa are serious threats to the society‚ causing minor to major health afflictions. Certain medicines
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different bacterial species have been isolated from fish-processing environments‚ e.g. Exiguobacterium spp. has been isolated from a fish processing plant in Japan (Yumoto et al.‚ 2004) and Staphylococcus spp. in food is often associated with human contact. Furthermore‚ due to the halophilic nature of Staphylococcus spp. (Simon
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GRAM STAINING EXPERIMENT CONDUCTED ON 9/29/2013 Introduction: The Gram stain is a useful stain for identifying and classifying bacteria. The Gram stain is a differential stain that allows you to classify bacteria as either gram positive or gram negative. This gram stain technique was discovered by Hans Christina Gram in 1884. The gram stain procedure separates all bacteria into one of two groups - into gram-negative bacteria which do not stain purple and into gram-positive
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MacConkey test was then used to confirm these results‚ since it has the ability to select for gram negative bacteria (Figure 9). With negative results for this test‚ a variety of bacteria were able to be eliminated from the list of bacteria‚ including Staphylococcus aureus‚ Pseudomonas aeruginosa‚ and Enterobacter aerogenes. The EMB test (Figure 10)‚ helped to further eliminate more bacteria including E. Coli. This confirmed our results along with the negative results for the Indole test and the Sulfur reduction
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