MICROBIOLOGY SECTION The microbiology section aims at studying yu ailments and diseases from the isolation of suspected causative organisms. The processes involved in the isolation of these organisms include; culturing‚ staining‚ microscopy and sensitivity tests. Samples collected for examination include; stool‚ blood‚ sputum‚ urine‚ vaginal swab‚ wound swab and wound biopsy. Bacterial Culturing and sensitivity Cultures are carried out to isolate suspected organisms from a sample. There are different
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Components of the Respiratory System 5) Which of the following statements about normal microflora of the respiratory system is incorrect? a) The presence of Haemophilus in the pharynx does not indicate disease. b) About 1/3 of the population carries Staphylococcus aureus in their nasal cavity. c)
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#3. Name the types of microscopes. Simple Microscopes Compound Microscopes Scanning Electron Microscope Transmission Electron Microscope #4. Identify these stains: (Functions and Reagents) Simple Stain- CULTURES: Staphylococcus epidermis slant Bacillus megaterium broth MATERIALS USED: Methylene blue‚ Distilled water‚ Slide‚ Inoculating Loop FUNCTION: To observe shape size‚ morphology‚ and arrangement. ---FROM SOLID: (slant) 1. Mark the smears on the underside
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Membranous organelles X Cell wall X Divide by mitosis X Divide by binary fission X Activity Page 4 Sample Name Physical Description (What does it look like?) Example species Gram-positive cocci in clusters Little groups of purple scattered Staphylococcus Aureus Gram-positive cocci in chains Chains of purple dots Streptococcus
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148: 14–23. 27. Bexfield A‚ Nigam Y‚ Thomas S‚ et al. Detection and partial characterisation of two antibacterial factors from the excretions/secretions of the medicinal maggot Lucilia sericata and their activity against methicillin-resistant Staphylococcus aureus (MRSA). Microbes Infect‚ 2004‚ 6: 1297–1304. 28. Bonn D. Maggot therapy: an alternative for wound infection. Lancet‚ 2000‚ 356: 1174. 29. Horobin AJ‚ Shakesheff KM‚ Pritchard DI. Promotion of human dermal fibroblast migration‚ matrix remodelling
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as in colonization and infection by drug-resistant organisms. Treatment regimens for all infections should be based on susceptibility test results when available. There are bacteria that require contact precautions such as Methicillin-resistant Staphylococcus Aureus (MRSA)‚ vancomycin resistant enterococci(VRE)‚ extended-spectrum beta-lactamses(ESBL)‚ Acinetobacter baumannii‚ Clostridium difficile (C-diff)‚ carbapenemase-producing enterobacteriaceae (CRE)‚ and multidrug-resistant bacteria (MDR). We
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Human Hepatitis C In 2013‚ there were an estimated 29‚718 cases of acute hepatitis C virus infections reported in the United States. There were 3.5 million people who were reported to have chronic hepatitis C. Acute hepatitis C is a short-term illness that occurs within the first six months after someone is exposed to the hepatitis C virus. Chronic hepatitis C means the virus remains in the person’s body. Hepatitis C can last a lifetime and lead to cirrhosis or scarring of the liver or liver cancer
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Microbiology 214BA Dr. May June 6‚ 2012 Staphylococcus aureus- Is a facultative anaerobic‚ Gram-positive‚ salt positive‚ cocci shaped bacterium. Staphylococcus aureus is found as normal part of the skin floral in the nasal passages and on the skin. An estimated twenty percent of people naturally have harmless Staphylococcus aureus on their skin and are long-term carries for Staphylococcus aureus. Staphylococcus aureus is the most common strand of Staphylococcus in humans to date‚ spread through skin
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antibacterial activity of the green synthesized silver nanoparticles from aqueous extract of Gum of fresh Coconut was tested by the disc diffusion method against an antibiotic‚ ampicillin(1µg/µl) as a standard drug. The antibiotic resistant bacteria viz.‚ Staphylococcus aureus‚ Bacillus subtilus‚ Escherichia coli and Klebsiella pneumonia were used for the measurement of antibacterial activity‚ and the plates were incubated at 370C overnight to know the zone of inhibition. 2.2. Cell Culture and Nanoparticles
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Oxygen Requirements of Bacteria BACKGROUND The GasPak system is useful for culturing anaerobic bacteria on standard microbiological media because the GasPak generates carbon dioxide and hydrogen. The hydrogen will combine with oxygen present in an anaerobic jar to produce water. This system can reproducibly attain oxygen levels in the parts per million range if used correctly. This is the best method for determining the oxygen requirements of unknown organisms. A candle jar is useful
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