Austin Peay State University Department of Chemistry CHEM 1021 IDENTIFYING CARBOHYDRATES (adapted from Blackburn et al.‚ Laboratory Manual to Accompany World of Chemistry‚ 2nd ed.‚ (1996) Saunders College Publishing: Fort Worth) Purpose: To become familiar with some of the characteristic reaction of carbohydrates. To identify an unknown carbohydrate. Caution: Wear eye protection because of the possible shattering of dropped glassware and because acids are used in this experiment
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with iodine and is oxidised to dehydroascorbic acid rapidly in acidic medium. first Considering iodine is only slightly soluble in water‚ the above titration cannot be performed directly by a standard iodine solution because of the insignificant end point. Thus‚ alternative method‚ back titration‚ will be employed. Two equations are involved. First‚ standard potassium iodate(V) (KIO3)‚ potassium iodate(KI) and dilute sulphuric acis(H2SO4) react to find out the excess quantity of iodine. Below
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The first lab will investigate the movement of glucose and starch across a selectively permeable membrane through a process of diffusion. The movement of a solute through this membrane is called dialysis . Diffusion is a form of passive transport. It is the movement of molecules from areas of higher concentration to those of lower concentration‚ until there is an even concentration. This movement is random because it is a result of kinetic energy. Diffusion is a slow process‚ but there are many factors
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as the optimal temperature needed for the enzyme to correlate with the bacteria and fungi. The enzyme’s break down within the starch was observed through different temperatures and time periods. The Starch was placed in both the fungal and bacterial amylase where they were then placed on spot plates. Through the iodine test‚ it was concluded whether the breakdown of starch occurred or not. The experiment
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BIOLOGICALLY IMPORTANT MOLECULES Abstract Compounds containing the element carbon‚ called organic compounds‚ are the most important substances that make up living organisms. There are thousands and thousands of different organic compounds. To identify the major types of organic compounds in living organisms ‚ several biochemical test were used . Introduction Organic molecules are those primarily made up of carbon‚ hydrogen and oxygen. The common organic compounds of living organisms are
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Who Took Jerell’s iPod? Lab Purpose: Standardized Tests The purpose of testing the organic nutrients (vegetable oil‚ glucose‚ starch‚ powdered egg white) with each of the different indicators (brown paper towel‚ benedicts‚ iodine‚ biurets) was to determine the color it would turn with positive traces of the nutrient. The purpose of testing just the Distilled H2O was to determine the color it would turn with negative traces. Nutrient in the Different Foods The purpose of testing each of the
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of the enzyme diastase and investigate its effect on the rate of starch digestion. The rate will be determined by the amount of time it takes to completely digest the powdered starch‚ the complete digestion will be indicated by the color change of the solution by iodine. Hypothesis: My hypothesis is that an increase in enzyme concentration will cause the faster digestion of starch. This means that it will increase the rate of starch digestion. Background information explains that substrates bind
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CHM130 Lab 3 Identification of Chemical Change Name: Paige Miller A. Data Table (24 points) Place your completed data table here: Reactants Predictions Before Combining Observations After Combining Starch + I2 I2 will turn Starch black I2 turned starch black Food coloring + NaOCl NaOCI will turn blue Coloring and NaOCI did not combine‚ NaOCI separated to the bottom Food coloring + CH3COOH CH3COOH will turn blue CH3COOH darkened the blue color Food coloring + NaOCl + CH3COOH Will
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CSBE – Class 12 – Biology – Experiment 12 Experiment No. 12 Aim- To study the effects of temperature on the enzymatic activity of salivary amylase on substrates starch. Materials required- Test tubes‚ beakers‚ pipettes‚ funnels‚ thermometer‚ cotton‚ starch‚ iodine‚ potassium iodide‚ sodium chloride‚ thermocol box‚ buffer solution of pH 6.8 & match box. Principle- Salivary amylase is an amylolytic enzyme found in saliva released from salivary glands. All the enzymes show optimum enzymatic activity
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Joanne Kathleen Saori T. Suzuki‚ Laurent Santos‚ Val Justin D.M. Tongco* College of Science‚ University of Santo Tomas‚ Manila Philippines Abstract Salivary amylase‚ found in humans‚ is enzyme that catalyzes the hydrolysis of starch into simpler compounds. Its enzymatic activity is affected by several factors‚ such as temperature and pH. The rates of enzymatic activity of salivary amylase in different temperatures and pH were measured and resulted to be very near 50 C and 7 respectively. However
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