does not change. 4. Hypothesis: I think because oil is so thin and has a yellow tint it is substance D based on my research. If substance D is the lipid then I would guess that substance A is the sugar‚ substance B is the protein and substance C is starch. 5. Materials: Supplies Equipment Chemicals 1
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Amylase. Solutions of starch and amylase were held at selected temperatures by various methods of temperature control. Once the solutions reached and maintained the desired temperature they were combined. Samples at timed intervals were then taken and reacted with a reagent to determine the effect the selected temperatures had on the reaction rate of enzyme and substrate. Results indicated that the enzyme functions efficiently at its optimum temperature (50oC) digesting the starch present and that any
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then the gelatin will change color. Hypothesis F1: If you add Benedict’s solution to the urine sample and boil it‚ then it will change color‚ indicating that glucose is present. Hypothesis F2: If you add iodine to the urine sample‚ then it will turn color‚ stating that there is a starch present. Hypothesis F3: If you add Biuret’s solution to the patient’s urine sample‚ then it will change color‚ meaning a protein‚ or child‚ is present. Hypothesis F4: If you put a drop of the urine sample on
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Amylase breaks down the biological macromolecule‚ carbohydrates‚ specifically starch into condensed subunits categorized as monosaccharaides or disaccharides. Two types of variables were human and fungal Amylase. Sum of six trials for both variables were conducted with two minutes intervals was measured for the duration. After every two minutes‚ the components were taken out of their controlled environment and joined with Iodine to observe visual change. The results gave supported facts that human amylase
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Proteins Lipids And Nucleic Acids Professor Anthony Depass Biology 1 9/25/14 Introduction: By completing lab exercise 6 : Biologically Important Molecules‚ we are able to perform tests such as the Benedicts Test for Reducing Sugars‚ Iodine Test‚ Biuret Test‚ Sudan IV Test‚ and lastly the Grease-Spot Test that detect the presence of biologically important carbohydrates‚ protein lipids and nucleic acids. Most known compounds in living organism are if fact carbohydrates‚ lipids‚ proteins
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They act as storehouses of chemical energy (glucose‚ starch‚ glycogen); are components of supportive structures in plants (cellulose)‚crustacean shells (chitin)‚ and connective tissues in animals (glucosaminoglycans); and are essential components of nucleic acids (D-ribose and 2-deoxy-D-ribose). Monosaccharides‚ oligosaccharides‚ and polysaccharides are theclassifications of carbohydrates. This experiment involves the carbohydrates starch‚ glucose‚ fructose‚ maltose‚ xylose‚ and sucrose. These
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The Effect of Temperature on Animal and Fungal Amylase’s Ability to Breakdown Starch. Abstract This experiment was designed to test the reaction of the enzyme amylase at various temperatures. There were two different kinds of amylase being tested‚ one was fungal amylase also known as aspergillus oryzae and human amylase. The changes in temperature effect the rate at which an enzyme and a substrate collide. When the temperature is too high the active site changes shape or denatures‚ once this
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Lecturer’s name: Mr. Paul Davidson Discussion The name of enzyme involved in this experiment is salivary amylase‚ which presents in our saliva. Salivary amylase catalyses the breakdown of complex sugar‚ such as starch‚ into a simple sugar‚ such as glucose. In this experiment‚ the optimum temperature for salivary amylase to function is 37˚C. Above the optimum temperature‚ the kinetic energy in the substrate and enzyme increases. The number of collisions between
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What is the Winkler Method? The Winkler Method is a technique used to measure dissolved oxygen in freshwater systems. Dissolved oxygen is used as an indicator of the health of a water body‚ where higher dissolved oxygen concentrations are correlated with high productivity and little pollution. This test is performed on-site‚ as delays between sample collection and testing may result in an alteration in oxygen content. How does the Winkler Method Work? The Winkler Method uses titration to determine
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the best methods is the iodine-thiosulfate titration procedure. Iodide ion‚ I-‚ is easily oxidized by almost any oxidizing agent (It has many electrons to lose!). In an acid solution‚ hypochlorite ions oxidize iodide ions to form iodine‚ I2. The iodine that forms is then titrated with a standard solution of sodium thiosulfate. The analysis takes place in a series of steps: 1.Acidified iodide ion is added to hypochlorite ion solution‚ and the iodide is oxidized to iodine. 2H+ (aq) + ClO-(aq) +
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