Abstract This experiment was carried out to monitor the ability of the Barley Amylase Enzyme to effectively break down starch in solutions that are increasing in neutral pH. To do this the experiment was carried out so that tubes containing a reaction solution of the Amylase enzyme and starch were simultaneously mixed. The reactions were then introduced to I2-KI‚ which stopped the reactions‚ at two minute intervals. Each of these trials was repeated three times to ensure proper accuracy. After
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I. Title: Analysis of a Commercial Bleach II. Purpose: III. Materials: Commercial bleach‚ 5% NaClO. (5 mL). Hydrochloric acid (HCl)‚ 3M. (6 mL). Potassium Iodide (KI). (6 g). Sodium thiosulfate solution (Na2S2O3)‚ 0.100 M. (70 mL). Starch solution 2%. (3 mL). Safety goggles Apron Digital scale Buret Buret clamp Erlenmeyer flask Pipet bulb Ring stand Ttransfer pipet‚ 5 mL and 25 mL Volumetric flask‚ 100 mL Stopper Wash bottle Distilled water Weigh boat Beakers‚ IV. Procedures:
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Laboratory ReportTitle: Observations of Chemical ChangesDate: 9/9/13Name: Purpose: The purpose of this experiment is for the student to gain knowledge and experience regarding how avariety of chemicals react to one another. In addition‚ the student should also gain a betterunderstanding of the household chemicals that they use in their day to day lives.Procedure:Observe the chemical changes that occur by mixing a list of chemicals (provided in the lab manual).Add 2 drops of the first chemical
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hyperthyroidism‚ menopause and lung disease can cause excessive sweating. The most common symptom people with hyperhidrosis is wetness. There are several test a doctor can perform to test for this. The starch-iodine test consist of an iodine solution being put on the area. After it dries‚ starch is then put on it and if there is excessive sweat‚ the area will turn blue. The paper test consist of a special paper being put on the area. The more the paper weighs‚ the more excessive sweat there is.
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To determine if the reaction had gone to completion‚ a Starch/Iodine test was conducted. A change in the strip to a deep blue color would signal an excess of sodium hypochlorite reagent present. An excess is the reagent symbolizes the end of the reaction because there is no longer any reactant left to consume reagent. After the reaction was deemed completed‚ the product was isolated and purified by ether extraction‚ aqueous extraction and evaporation. To determine how sodium hypochlorite acts as
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Dialysis section is divided into two parts: test for copper(II) ion and test for starch. For Test for Copper(II) ion part‚ take 1 mL of the water from the dialysis beaker and place it in a test tube‚ and then add a few drops of concentrated ammonium hydroxide and record the observations. Lastly‚ for Test for Starch section‚ take 1 mL of the water from the dialysis beaker and place it in a test tube. Add a few drops of iodine solution and record
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how the concentration of Iodine changes with time and how varying concentrations of KI and H2O2 solutions affects the rate of reaction. 2 - Use an iodine clock: add 2cm3 of 0.1moldm-3 sodium thiosulphate (Na2S2O3) and 1cm3 of 1% starch solution at the start of the reaction. The added sodium thiosulphate reacts with the iodine as it’s formed: I2 + 2Na2S2O3 -> 2NaI + Na2S4O6 When the sodium thiosulphate is all used up the iodine remains in solution and reacts with the starch indicator to make the blue
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have become a huge importance to industry and sparked a large interest into the exploration of enzyme activity in microorganisms. Amylase is one of the most widely used enzyme required for the preparation of fermented foods. Apart from food and starch industries‚ in which demand for them is increasing continuously‚ amylase is also used in various other industries such as paper and pulp‚ textiles‚ and medical labs. The global market for enzymes was about $2 billion in 2004 (Sivaramakrishnan et
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that Mio and other flavor drops perform diffusion when put in water as an example. For the lab part we put a mixture of starch and water into a plastic Ziploc bag and submerging it into a solution of water and iodine. The bag was semi-permeable and so some of the iodine leaked through into the bag showing osmosis. The iodine‚ being the indicator‚ changed the color of the starch mixture to have streaks of light gray with a purple tint almost. We also learned that molecules tend to move from areas
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OBJECTIVE: 1. To distinguish the bacteria abilities to metabolize various substrates and end products formed. 2. To observe the growth of different bacteria species in term of structures and its morphology based on different chemical substance applied. 3. To observe physiological and immunological properties utilized by different species of bacteria. INTRODUCTION: Bacteria biochemical testing can determine the types and numbers in terms of colony forming units of bacteria present in a
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