Aim What is the effect of Lead ions on the enzyme Amylase. And does it have an inhibitory effect‚ which causes the substrate‚ in this case starch to be blocked from the reaction process in the enzyme catalyst. Also is the effect reversible or irreversible‚ which is put on the amylase. Method Apparatus and substances required Test tube holder 2% starch solution 6 boiling tubes labelled 1 to 6 1% lead nitrate solution 6 test tubes labelled A to E‚ ( F) Dimple tiles 5 test tubes labelled A1
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clean” and “Magic power”. Principal: Amalyse can catalyse the breakdown of starch into maltose. In this practical‚ solutions of the 2 washing powders will be filled into 2 identical wells on the starch agar plate separately. Starch will be broken down by the amylase disused to the star-agar. A clean zone will be formed around the wells when iodine solution is added and flushed. The higher the amylase activity‚ the more the starch will be broken down. Hence‚ a larger and clearer zone will be observed
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electrons from aldehyde or ketose group of reducing sugars to form cuprous oxide‚ a red brown precipitate. Materials: The materials used in order to detect starches was: test tubes‚ distilled water‚ Benedict’s solution‚ starch were used. Negative Control: H20 Positive Control: Starch Obtain nine test tubes and number them 1-9 Add to each tube the materials to be tested. Add 2ml of Benedict’s solution to each tube. Place all the tubes in boiling water- bath for 3 minutes and observe color changes
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BIOPLASTICS Bioplastics are a form of plastics derived from renewable biomass sources‚ such as vegetable fats and oils‚ corn starch‚ pea starch or microbiota. There are a variety of materials bioplastics that can be composed of‚ including: starches‚ cellulose‚ or other biopolymers. APPLICATIONS OF BIOPLASTICS Biodegradable bioplastics are used for disposable items‚ such as packaging and catering items (crockery‚ cutlery‚ pots‚ bowls and straws). They are also often used for bags‚ trays‚ containers
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Abstract This lab was focused on determining the optimal temperature of the enzyme amylase responsible for catabolizing starch polymers and to see how different temperatures affected the rate as well as how effectively the enzyme worked. To proceed with the experiment the group set up four different test tubes for each‚ bacteria and fungal amylase‚ and labeled them accordingly with different temperatures as well as different solutions . Then the spot plates were placed on the time and temperature
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precipitate. A non-reducing sugar will be verified by a negative test of Benedict’s solution before hydrolysis by acids‚ followed by a positive test of its hydrolysis into monosaccharide components. The iodine test is done to identify glycogen and starch. The polysaccharides combine with the iodine which will give out a black-blue coloration. There are two tests that are commonly used to test for protein which are the Millon’s and Biuret’s test. A Millon’s test detects the amino acid tyrosine and
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molecules through dialysis tubing. We used the I2K1 because it is small enough to diffuse and the starch is too large to diffuse out. By detecting the presence of particular molecules on each side of the dialysis tubing‚ we determined which molecules passed through the tubing and which did not. When the starch molecules react with iodine to form a dark blue compound. We used the I2K1 to test for starch and the Benedicts test to test for reducing sugar. Based on the information my hypothesis for this
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follow the activity of amylase because it has a major role in the life of living organisms and is found abundantly in them. Amylase is a catalytic enzyme which hydrolyzes starch into maltose and dextrin at a certain temperature (Biology.kenyon.edu‚ 2015). In plants such as fruits and vegetables carbohydrates are referred to starch which is polysaccharide and is converted into disaccharide and eventually into monosaccharide such as glucose with the help of amylase. (Fullick‚ Locke and Bircher‚ n.d
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are enzymatic desizing and oxidative desizing. Acid steeping is a risky process and may result in the degradation of cotton cellulose while rot steeping‚ hot caustic soda treatment and hot washing with detergents are less efficient for the removal of the starch sizes. * Enzymatic desizing : Enzymes are solubilizing bio catalysts‚ mainly proteins‚ thermo labile (readily changed or desized by heat) and highly specific in their action. A
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together) Polysaccharide (made of many monosaccharides joined together) maltose = α-glucose + α-glucose sucrose = glucose + fructose lactose = glucose + galactose germinating seeds phloem tissue‚ fruit milk β-glucose fructose O maltose starch glycogen cellulose chitin = = = = polymer of glucose polymer of α-glucose polymer of β-glucose polymer of glucosamine (glucose with an amino acid attached) chloroplast stroma muscle cells plant cell wall exoskelteton of arthropods O O O O cellulose
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