University of Texas at Tyler Lab 3C: Purification of L-Lactate Dehydrogenase By Affinity Chromatography on Cibacron-Blue Sepharose David Alexander 10-15-2014 Dr. Black Chem 4135.001 Abstract: Like the previous experiments‚ the ultimate goal of this lab was to purify the enzyme sample. However‚ this is the last lab for purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally
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Instructor Biology 1111 4-5 Lab Topic 4: Microscopy Elodea Cells at ___X Elodea Cells at ___X Report Sheet—Lab Topic 4 1. Draw and label each of the organisms available. Cheek Cells at ___X Cheek Cells at ___X Name _______________________________ Date_____________ Instructor ___________________________ Section___________ _________________________ 4-6 Lab Topic 4: Microscopy 2. Fill in the following table: Compound Microscope Dissecting Microscope Types of Light Available Powers
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Submit your completed lab report to the Lab: Photosynthesis Lab assignment link for grading. For information on how this assignment will be graded‚ please visit the Course Information sectionChlorophyll and Accessory Pigments A pigment is any substance that absorbs light. The color we see comes from the wavelengths of light that reflect. Chlorophyll‚ the green pigment common to all photosynthetic cells‚ absorbs all wavelengths of visible light except green. The green reflects back to our eyes
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most effective at scarifying seeds to improve the rate of germination‚ and what concentration of the most effective chemical was the most efficacious. Ten seeds for each of the five chemicals hydrogen peroxide‚ glucose‚ hydrochloric acid‚ isopropyl alcohol and citric acid were soaked in the solution for approximately twelve hours then subsequently rinsed to remove residue of the chemicals. They were then planted in beds of cotton wool inside plastic containers
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Chemical Report The chemical I will be writing this report on is oxygen. The name oxygen comes from the Greek word “oxygens‚” which means “acid producer”. Dictionary.com defines oxygen as “a colorless‚ odorless‚ gaseous element constituting about one-fifth of the volume of the atmosphere and present in a combined state in nature.” Oxygen has many uses today. First‚ oxygen is used by people‚ plants‚ and animals in the respiration process. Tanks of oxygen are used in the healthcare field to treat
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Radial Immunodiffussion (RID) Christian Crespo 18 October 2013 Immunology Lab Report Purpose of the Experiment: The objective of this experiment is to quantitatively observe the foundational reaction in our Immune system; the Antigen-Antibody interactions. The Ouchterlony procedure is what will be used in this lab to detect nature of the antibody interaction. The orientations of the band will provide more information about the interaction of antibody and antigen
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Planarian Lab Report Hanna Klein Bishop Fenwick High School Planarian Lab Report Introduction Planarian worms are free-living flatworms that are not parasitic. Planarians have a three-cavity digestive system. Planarians are usually either carnivorous or scavengers. Planarians may eat other living‚ as well as dead‚ invertebrates‚ detritus or decaying organic matter. Planarians do not realize that are eating a lot‚ and can over eat resulting in death. Most planarians are freshwater forms that can
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Bacterial Transformation Lab Report Backround: The plasmid pGLO contains an antibiotic-resistance gene‚ ampR‚ and the GFP gene is regulated by the control region of the ara operon. Ampicillin is an antibiotic that kills E. coli‚ so if E. coli‚ so if E. coli cells contain the ampicillin-resistance gene‚ the cells can survive exposure to ampicillin since the ampicillin-resistance gene encodes an enzyme that inactivates the antibiotic. Thus‚ transformed E. coli cells containing ampicillin-resistance
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Chromatography lab Purpose: To separate food colorings into their component dyes using paper chromatography. Materials: Chromatography paper‚ Food coloring‚ Ruler‚ Pencil‚ Solvent solution‚ Test tubes‚ Test tube rack. Safety precaution: wear aprons‚ to make sure that you don’t get any of the alcohol on your clothes‚ and if you break a test tube you don’t get glass on you. Procedure: See-attached handout. Results: See chromatography with Audrey’s lab report.
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Introduction The purpose of this lab was to calculate and determine the optimal dosage of ultraviolet (UV) light to expose Saccharomyces cerevisiae (S. cerevisiae) to that would result in a survival rate of 20-40%. Saccharomyces cerevisiae commonly referred to as baker’s yeast is a single-celled eukaryote. Yeast is capable of growing as either a haploid or a diploid cell. The diploid cell can reproduce mitotically or sporulate‚ a process initiating meiosis and forming an ascus with four spores (Sacchromyces
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