Blue white selection is a widely used method in screening recombinants in cloning. This is based on the gene product of lac z gene. The plasmid vectors contain this gene which produces β galactosidase enzyme. When a gene is inserted close to lac z gene‚ the reading frame will be distorted and the gene is inactivated. So the transformed cells will not produce this enzyme and are called competent cells. After the recombination‚ the bacterial cells are grown in a medium containing X gal (5-bromo-4-chloro-indolyl-β-D-galactopyranoside)
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quickly spread to the other wet-nurses and servants—she passed from hand to hand in a succession of fleeting maternity‚ leaving her swaddled in mourning clothes. They preferred to keep the child covered‚ for even a fleeting glance of her chilled milk-white skin and blood stained lips created a deathly shiver. The child was hungry too‚ it was said; what stores of food the kingdom retained were never enough‚
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The story White Angel is one of a defining moment. Bobby Morrow‚ the focal character‚ remembers in great detail his life as a nine year old in the late 1960’s‚ and how his brother’s death changed his life completely. Bobby and his sixteen-year-old brother Carlton do everything together‚ and Bobby looks to Carlton as something of a guardian angel or god. In reality though‚ Carlton leads Bobby to a life of drugs and risk. Eventually‚ Carlton’s risky behavior catches up with him‚ and leads him
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Balram finds that the liberated individuals have set up innumerable technology outsourcing companies in the India of Light that “virtually run America now” (The White Tiger 4). He lacks the education or business acumen to build a technology start-up. Instead‚ he puts his driving skills‚ knowledge of cars and drivers and the capital he has stolen from Ashok to good use by setting up a business that serves the need of the burgeoning IT industry. He provides them with unmatched logistical solutions
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Dead White Males Essay Dead White Males is an Australian play written by David Williamson. Throughout the play‚ the two ideologies Liberal Humanism and Post Structuralism are represented and hotly debated. The debate of these two ideologies had been the cause of many conflicts and drama from the beginning to the end of the play‚ and characters Dr Grant Swain and Shakes were the main characters behind this turmoil. Swain believes in the ideology Post Structuralism‚ and he is a university literary
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Bhullar 1 Aziz Bhullar" Jaqueline Seydel" English 10" 29 May 2014 " The Survival of Tomiko Higa" " Tomiko Higa is the author of the book The Girl With the White Flag. The book depicts the realities of war through the eyes of the protagonist‚ who is a little girl known as Tomiko. Tomiko lives on the island of Okinawa and resides with her family. When the war of Okinawa starts‚ she is separated from her family and struggles to survive. She has to forage for food‚ shelter‚ and her lost
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Anthropology November 20‚ 2012 The White Tiger Response The White Tiger is a brilliant collaboration of wit and darkness. The main character‚ a servant of his own kind‚ is a witty and somewhat likable killer from the underbelly of India. You can not help but enjoy this killer while also being disappointed with this character. Blaram’s story brings up a truth about Indian culture‚ as well as one- third of the world’s population who suffer from poverty. He explains the white world and the dark world
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me an American. Even before setting foot in Israel three years ago‚ I knew exactly where I could find one. The tiny T-shirt shop in the central block of Jerusalem’s Ben Yehuda Street did offer other designs‚ but the one with a bright white “Drink Coca-Cola Classic” written in Hebrew cursive across the chest was what drew in most of the dollar-carrying tourists. While waiting almost twenty minutes for my shirt (depicted in Fig. 1)‚ I watched nearly every customer ahead of me ask for “the
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I chose to do my audience analysis on Philippa Gregory’s books The White Princess. The book is a historical fiction book based on the lives of King Henry the 7th and Elizabeth of York. The primary audience for this book is for people who are interesting about the history and the people of the British monarchy. The reason that I believe that this is the primary audience for this book is because most people do not want to learn about Elizabeth of York unless they are interested in her and her life
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Dorothy Parker’s "Arrangement in Black and White" is set during a dinner party for the host’s friend‚ Walter Williams‚ an African American musician. Though the party is celebrated in his name‚ most of the conversation takes place between the host and the main character‚ the woman with pink velvet poppies. From the conversation‚ the audience can deduce that though this woman admires Walter Williams’s musical talent‚ she is unable to let go of the racist sentiment against his African American heritage
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