The total protein content was estimated as proposed by Lowry et al. A bovine serum albumin stock solution (1mg/ml) was prepared in sodium hydroxide (1N). Five different concentrations (0.2‚ 0.4‚ 0.6‚ 0.8‚ 1 ml) of the prepared solution were taken in different test tubes. In another set of test tubes‚ 0.1 and 0.2 ml of the extract were taken. In each test tube‚ the volume was made up to 1 ml‚ followed by addition of the prepared alkaline solution (5 ml) at room temperature. The solutions were left
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purpose of this lab was to find the theoretical and experimental percentage yields of the double displacement reaction between the solutions Lead (II) Nitrate (PbNO3) and Potassium Iodide (KI). It is important to obtain amounts of Lead (II) Nitrate and Potassium Iodide as close to 1.44g as possible. This reaction creates Lead (II) Iodide and Potassium Nitrate. The precipitate during this reaction is Lead (II) Iodide. The balanced equation is Pb(NO3)2 + 2KI= PbI2 + 2KNO3. In this lab the Lead (II) Iodide
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Concentration vs Rate of reaction Brandon Introduction A reaction rate is the speed at which a chemical reaction will occur. Heat‚ concentration of a chemical‚ Surface area or a catalyst‚ will affect the rate of reaction. The concentration of a chemical will speed up of the reaction because the reactant particles have become more crowded therefore increasing the chance of productive collisions. In this practical the chemicals will be Sodium thiosulphate and Hydrochloric acid which will produce
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Physical and Chemical Reactions Lab #4 Alesa Cannon Objective: To investigate the physical and chemical properties of a pure chemical substance. Procedure: 1. Half-fill one well of a 24-well plate with 6 M HCl and half-fill a second well of the 24-well plate with 6 M NaOH. Suck up these chemicals into their labeled pipets for later use. 2. Perform the following steps on each of the substances to be tested. Complete all tests of one substance and record your observations before proceeding
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It was originally hypothesised that the reaction time would decrease with higher concentrations of the reactants‚ potassium iodate and sodium bisulfite as collision theory stated that more molecules present in a given volume meant a greater chance they would collide. Furthermore‚ the time was expected to decrease with higher temperatures as it influenced the reactant particles by raising their kinetic energy. However‚ the data collected from this experiment only partially supported this hypothesis
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The procedure was taken from “From Drosophila cDNA in E. coli plasmid to homologous human proteins” lab manual (4). - Colony Picking: Two E. coli colonies were grown on agar plates and treated with ampicillin. They contained the plasmid with genes for ampicillin resistance and Drosophila cDNA sequence. - Plasmid Isolation: We used the QuickLyse Miniprep Plasmid DNA purification systems to isolate the plasmid DNA. Indeed‚ the bacterial cells were removed from the liquid broth and were resuspended
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The purpose of this lab was to find out the reaction of iron nails and CuSO4‚ copper sulfate solution. Before starting the experiment‚ there were two possible outcomes of the reactions. CuSO4 + Fe → FeSO4 + Cu or 3CuSo4 + 2Fe → Fe2(SO4)3 + 3Cu. The ion chart showed that iron can only make two charges‚ +2 and +3. As the liquid evaporated‚ the weight changed because the liquid added to the nail’s weight. In a real world situation‚ scientists can use ratios to determine how much of a substance that
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Constant of an Unknown Ester Hydrolysis Reaction Abstract The experiments to follow determined that the equilibrium concentrations of the reaction: ester + water ↔ alcohol + acid‚ are equal to 0.0363 moles of ester‚ 0.2852 moles of water‚ and 0.0268 moles each of alcohol and acid. Using this information the equilibrium constant was determined to be 0.06938. 1. Introduction In this lab the equilibrium constant‚ Kc‚ for the acid catalyzed reaction between an unknown ester and water
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Good Morning‚ today I xxxx am here to talk to you about ‘’The Earth” Let me begin by a famous quote- “ The Earth provides enough to satisfy every man’s needs‚ but not every man’s greed.” ― Mahatma Gandhi The urgent message is “Save the Earth”. It’s a message that has been heard before and consistently being spread throughout the world. In this time that I have to talk to you‚ I want to show to you‚ how deep a mess we are in‚ with environmental crisis and how we can get out of it. It warns us
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For those of you who came to the review in class today‚ The stoichiometry question from class. I read the answer to the wrong decimal place. --What is the total mass of oxygen in 0.26mol of Cu2O? Find the percent of oxygen in Cu2O first. The molar mass of Cu2O is 143‚ the mass of just the oxygen in it is 16. % Composition of oxygen would be 16/143 times 100 is 11.19%‚ To determine the mass of Cu2O in 0.26 mol‚ multiply by the molar mass of Cu2O. This gives
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