Roy Levin Bio 11 Lab Dr.Izquierdo Analysis of Macromolecules in Tissue Homogenates of Bos taurusMaterials and Methods The homogenates provided were made by homogenizing tissues in a sucrose phosphate buffer in a 1:20 ratio. The protein concentration in bovine cells was measured by diluting the homogenate with a 1:5 ratio; 50 microliters of homogenate and 200 microliters of water. Then 5 known protein concentration samples which were 0.4‚ 0.8‚ 1.2‚ 1.6‚ 2.0 mg/ml of bovine serum were used to
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Chemestry Lab Rates of Reaction (Picture from: http://sciencespot.net/Pages/kdzchem.html) The rate of reaction is how fast or slow a reaction is completed. This is important for factories and chemists all over the world. What happenes during a reaction is atoms or molecules that collide and form new molecules. You can affect this rate of reaction with: temperature of reactants or suroundings‚ surface area of reactants‚ if there is a catalyst present‚ but in theis lab the concentration was changed
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dissolving the para-aminonphenol hydrochloride salt in water‚ decolourising the aqua solution and crystallising the para-aminophenol by neutralizing the salt. The resulting para-aminophenol is reacted with acetic anhydride to produce paracetamol. The reactions are summarized as shown below: Process using Paranitrochlorobenzene as a starting raw material Paranitrochlorobenzene is converted to paranitrophenol by means of caustic fusion with caustic soda in an autoclave; paranitrophenol thus
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isomers of a typical coordination compound. INTRODUCTION: Isomerism played a central role to establish the basic concept in coordination chemistry while reaction kinetics of coordination compound is known as the area of continuing intense research activity. The mechanism of the reactions of coordination compounds relevance to enzyme reactions where the active site is being involve in coordination to a metal ion. PROCEDURE: (A) Preparation of Trans-Dichlorobis(ethylenediamine)cobalt(III) chloride
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percent yield of the final product is determined after the synthesis. Procedures: 2.0 mL of 1-bromobutane‚ 3.0 g of sodium benzoate‚ 5.0 mL of water‚ 4 drops of Aliquat 336‚ and a boiling stone were placed in a 50mL round-bottomed flask. The reaction mixture was refluxed for 1 hour and the flask was cooled in a beaker in the water of room temperature. The solid was formed in the mixture and the flask was shaken until it dissolved. The flash was rinsed with 15 mL dichloromethane and it was
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Radial Immunodiffussion (RID) Christian Crespo 18 October 2013 Immunology Lab Report Purpose of the Experiment: The objective of this experiment is to quantitatively observe the foundational reaction in our Immune system; the Antigen-Antibody interactions. The Ouchterlony procedure is what will be used in this lab to detect nature of the antibody interaction. The orientations of the band will provide more information about the interaction of antibody and antigen
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Wave Nature of Light Objective: The purpose of this lab is to investigate interference‚ otherwise known as the diffraction of light. A beam of light acts a wave‚ and we are able to use equations so calculate the wavelength of the light used. The diffraction of a straight edge demonstrates that light waves bend around straight edges‚ allowing light to enter an area of shadow. When waves are superposed‚ they reinforce each other when crests are in phase and cancel out when they are not in phase
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PEES 4200W- Physiology of Exercise Lab #2: Metabolism and Energy Expenditure 1. The Respiratory exchange ratio is the ratio of the volume of carbon dioxide produced and oxygen consumed. The ratio indicates the energy that the subject is expending for indirect calorimetry‚ how efficient the subject ’s body is at utilizing the oxygen inhaled‚ as well as the main substrate being used for energy during varying intensities of exercise. During rest the volume of carbon dioxide was 0.73L/min‚ the volume
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LSM1102 Lab Report Introduction Transformation is a process which involves plasmid DNA being bound to the cell surface and the subsequent uptake of DNA by the cell (Panja et al.‚ 2008). For artificial transformation of E. coli cells with plasmids‚ plasmid DNA has to be extracted from bacterial cells using the High-Speed Plasmid Mini Kit‚ which is then mixed with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin)
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Introduction The study of chemical reactions‚ isomerizations and physical behavior that may occur under the influence of visible and/or ultraviolet light is called Photochemistry. The fundamental principles for understanding photochemical transformations are that light must be absorbed by a compound in order for a photochemical reaction to take place‚ and that for each photon of light absorbed by a chemical system only one molecule is activated for subsequent reaction. This "photo equivalence law" was
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