Enzymes are biological catalysts or assistants that consist of various types of proteins that work to drive the chemical reaction required for a specific action or nutrient. They can either launch a reaction or speed it up. Catalase is a common enzyme found in nearly all living organisms exposed to oxygen. It is a very important enzyme in protecting the cell from oxidative damage by reactive oxygen species (ROS). The catalase used in this experiment will come from five different sources: Spinacia
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Purpose: The Purpose of this lab is to utilize‚ demonstrate and understand the various techniques and procedures used to gravimetric labs. For this particular lab we will utilize our scientific knowledge of related to gravimetric procedures to find the chloride content in an unknown soluble salt. Theory: Using our developed knowledge of the conservation of mass‚ solubility and precipitation it is possible (with some degree of error) to know the content of chlorine in a particular salt by dissolving
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concentrations of peroxidase and its reaction rate in seconds‚ we were able to see that as the amount of enzyme increased the catalytic reaction also increased. The optimal amount of peroxidase concentration to be used in the subsequent experiments was determined to be 1.0 mL. Any amount above this would have caused the rate of absorbance to be too fast‚ making it too difficult to get accurate readings. Any amount below this would not have produced a reaction “at an appreciable rate.” (Dolphin
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of pH affect the reaction rate of the enzymes in chicken liver? Demonstrate the activity of an enzyme in living tissue‚ observe the effects of changes in temperature and pH on the activity of an enzyme‚ perform analyses for the presence of an enzyme in tissues‚ and analyzing relationships between environmental conditions and enzyme activity. Background: Cells produce proteins which are called enzymes and their job is to help reduce the amount of energy needed to start a reaction. Enzymes are catalysts
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Unknown Lab Report Dr. Nathan Cahoone Microbiology 204 December 9‚ 2010 Introduction There are many reasons for knowing the identity of microorganisms. The study and test was done by applying all of the methods that have been learned so far in the microbiology laboratory class for the identification of an unknown bacterium which I was using unknown #25. Results Unknown #25 had the following morphology on a streak plate: medium sized butyrous cream colored colony. Gram-staining was utilized
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Session 1: In this lab‚ we will achieve a simple Friedel-Crafts alkylation of anthracene. The choice of anthracene as an aromatic substrate stems from two considerations. First‚ there is a question of regioselectivity. Second‚ anthracene and its derivatives are highly visible under UV light. Session 2: In this lab‚ we will complete a partial conversion of 9-acetylanthracene using m-chloroperoxybenzoic acid (mCPBA). We will also determine by NMR‚ the regiochemistry of the reaction. B. Chemical
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both the X and Y chromosomes in humans and can be used as a way to distinguish between males and females. Gender determination of a human has important applications in forensic science‚ prenatal diagnosis‚ etc. This can be done by Polymerase Chain Reaction (PCR) followed by gel electrophoresis. The presence of two bands of DNA indicates that the sample is from a male while the presence of one band of DNA indicates that the sample is from a female (sasaki and shimokawa‚ 1995). In this experiment‚ Chelex
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An enzyme is a tertiary globular protein. The function of an enzyme is to lower the activation energy of either the creation or breaking apart of a chemical bond. By lowering the activation energy of this process‚ the reaction of bonding‚ or in this case breaking apart‚ is sped up. An enzyme breaks apart the substrate in the active site of the enzyme; this is where the magic happens. Substrate is what is being broken apart by the enzyme. In this case‚ the enzyme is catalase and the substrate is hydrogen
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Abstract: Two parts of this lab were performed involving photosynthesis and cellular respiration. The first part of the lab consisted of cutting out spinach leaf disks with a straw and then putting them into syringes containing an infiltration solution and sodium bicarbonate. Then the syringes were place under the presence of light and watched as certain disks floated. This part of the lab consisted of watching photosynthesis take place. Then for the second part of the lab we tested cellular respiration
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Titration Lab Introduction The purpose of this lab is reach and be able to calculate the equivalence point when we use titration to neutralize a base with acid. The process of the lab was determining the volume of a solution needed to react with a given mass or volume of a sample is called titration. The equivalence point is when the same number of moles of acid and moles of base has been added. Phenolphthalein is used as an indicator because it will have a color change when the equivalence point
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