DNA Extraction from Wheat Germ and Making an Agarose Gel AIM: To be able to make and agarose gel and perform gel electrophoresis in six different dyes. Also‚ to extract DNA from wheat germ. INTRODUCTION: Agarose gel is a substance that is used in science for gel electrophoresis and size exclusion chromatography. These processes use agarose gel to separate and analyze proteins and DNA. The medium is composed of a purified agarose powder that has been boiled in a buffer solution and then cooled
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Can you expect to extract more DNA from a strawberry or kiwi fruit? Introduction: In the experiment we were inspecting the amount of genetic materials in fruits with different numbers of chromosomes. In the experiment‚ we mashed up a strawberry/kiwi fruit (See appendix 1 figure 2) added extraction butter and ethanol to extract the DNA. A chromosome is a long thread-like cluster of genes in the nucleus. DNA (deoxyribonucleic acid)‚ a self-replicating material which is present in nearly all living
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Title Application of DNA Barcodes to Identify Various Plant Species Abstract In this experiment we applied barcodes to plants in order to identify what species they are classified under. We also compared the DNA sequences of different plant species using the ribulose-biphosphate carboxylase gene (rbcL). We took samples from a plant called Chard and performed PCR‚ DNA amplification and quantification and sequenced the DNA. During the experiment‚ we hypothesized that this year’s “nonspinach”
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investigated the amount of DNA extracted from strawberries. This was done by using the independent variable of alcohol to affect the dependant variable of the amount of DNA extracted. This was done to find out if primary or secondary alcohol would create more DNA precipitate than the other. For this the primary alcohols used were; methanol and ethanol‚ and the secondary alcohol was; isopropyl. Of this the secondary alcohol‚ isopropyl was discovered to be the most effect alcohol to make DNA precipitate‚ as it
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DNA Extraction Lab Purpose: To compare the amount of DNA extracted from two different species‚ despite using the same method. Hypothesis: I predict that the liver will produce a higher quantity of DNA than the strawberry. This is because I believe that animals have a higher DNA yield because our structure is more complex than a plant’s structure. Materials: -Sample of Strawberries -Zip lock bag -DNA extraction buffer -Cold ethanol -Glass rod -Double ply cheese cloth -Two test tubes
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Procedure 2: DNA Extraction from Cheek Cells Materials: Water‚ Clear Dish Soap‚ Table Salt‚ Isopropyl Alcohol (70%) or Ethanol‚ Food Coloring 1. To 200 Ml drinking water add two teaspoons of salt 2. Gargle the salt water for 1 minute. 3. Spit the gargled water into a beaker (or new cup). Now your cheek cells are suspended in the salt water. 4. Gently stir the salt water with one drop of soap (try to avoid air bubbles) 5. In a separate beaker (or cup)‚ mix 20 ml isopropyl alcohol and 1-3 drops
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3.1 Source of the Recombinant DNA Polymerase SK72 The recombinant E. coli BL21 (DE3) containing the pET 32b/ DNA polymerase SK72 gene was provided by the Laboratory of Enzyme and Microbial Technology‚ Institute of Bioscience‚ Universiti Putra Malaysia. 3.2 Preparation of the Lysogeny Broth (LB) Agar Plates LB agar plates were prepared by dissolving 10g tryptone‚ 5g yeast extract‚ 5g NaCl and 15g bacterial agar in 950mL of deionized water. The pH of the medium was adjusted to 7.5 using 1M NaOH before
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Pay for one‚ get another absolutely free! You won’t fall for it if you saw it but this is incredible. 8. That’s a REALLY huge strawberry. Eating one of it would be like you had a proper meal! Damn‚ where do I find these sorts of things? 9. Looks like a hedgehog mated with a strawberry and their child turned out to be like this sprouting strawberry! 10. Why did this tomato have to mate with itself when other tomatoes were dying to mate with it? 11. Looks incredibly ridiculous
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1.0 Introduction The report “Business Plan of RedBerries Bangladesh” has been prepared to outline a business plan as a partial requirement for the course Entrepreneurship‚W201 instructed by Mr. Sheikh Morshed Jahan‚ Assistant Professor‚ IBA ‚ University of Dhaka. This report focuses on outlining an entrepreneurship venture into the strawberry cultivation industry of our country. This is a very new industry in our country‚ and while the growth has slowed down in the last years‚ this industry still
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Wild Strawberries What is the matter with Isak Borg and what can he do to change himself? Wild Strawberries tells the tale of an elderly doctor‚ Isak Borg. He thinks he has lived out a full and prosperous life but in fact he has lived his life somewhat devoid of emotional attachment by choice. He admits that some of his choices has brought him loneliness. The person who is closest to him is the maid who helps take care of him. He finally realizes that his life is not as complete as it should
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