2.4.4. Direct determination of saliva proteins Protein contaminated with nucleic acids absorbed the light at wavelength 280 nm and it absorbs much strongly at wavelength 205 nm when it is free from nucleic acids. The UV-visible spectrophotometer was used in determination of saliva proteins (Figure 2.2). Cold trichloroacetic acid (10 % w/v ) was added to the sample‚ centrifuged for 10 minutes to precipitate protein. The absorbance of a known volume
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Introduction This practical experiment was performed in an attempt to observe how enzymes (starch phosphorylase in particular) are affected by varying its temperature before introducing it to the substrate it will be reacting with. A catalyst (enzyme) is a substance that changes the rate of a reaction; for a reaction to take place at all‚ the enzyme must first come into contact with the substrate. Enzymes are subject to a number of factors which effect how fast they can cause a reaction with a substrate;
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sizes of molecules and how the chemical reactions take place. Therefore‚ the experiment was conducted using glucose and starch solution inside the dialysis tube. The starch and glucose that was put inside the dialysis tube help identify which of the two will reacted with potassium iodide inside the breaker‚ as the latter passed from the beaker into the tube‚ the glucose/starch solution’s change of color showed that the potassium iodide was small enough that it able to pass through from the solution
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Vol.3‚ No.6 (2009) 366–375 Available online at www.expresspolymlett.com DOI: 10.3144/expresspolymlett.2009.46 Starch-based completely biodegradable polymer materials D. R. Lu‚ C. M. Xiao*‚ S. J. Xu College of Material Science and Engineering of Huaqiao University‚ Quanzhou‚ 362021‚ P. R. China Received 21 February 2009; accepted in revised form 30 March 2009 Abstract. Starch is a natural polymer which possesses many unique properties and some shortcoming simultaneously. Some synthetic polymers
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PLANNING COURSEWORK- STARCH AND AMYLASE AIM The aim of this coursework is to investigate the effect of temperature change‚ on the rate of hydrolysis of starch catalysed by amylase. PREDICTION I think that as the temperature increases‚ the rate of reaction also increases‚ to a point when it dramatically decreases. On graph 1‚ you will see a sketch of the graph which I expect to be the result of the experiment. SCIENCE REASONING I think my prediction is correct because the rate of
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What amount of amylase is the best to produce a better reaction with starch? A 1% solution of amylase‚ made from 0.15g of amylase added to 15ml of water‚ a 2% solution of amylase made of 0.3g of amylase added to 15ml of water‚ and a 0.5% solution of amylase made of 0.75g of amylase to 15ml of water were created. A 0.25% starch solution was also created‚ and 3ml was added to each of the 4 test tubes. 1 drop of iodine was added to each test tube. 4ml of the 1% solution was added to the first test
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BIO 219 Group 1 Section 66 October 19‚ 2012 The extraction of purified DNA from A. fischeri by restriction digestion using Sal I enzyme and pGEM for shotgun cloning Introduction: The ultimate goal of this experiment is to isolate the lux operon‚ a targeted piece of DNA that causes bioluminescence‚ from Aliivibrio fischeri and insert it into the DNA of Escherichia coli in order to make it glow. A. fischeri is a gram-negative bacteria which participates in a symbiotic relationship with
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Title : Investigation of Action of Saliva and 3M HCl in Two Carbohydrates Solutions. Objective : To investigate the action of saliva and 3M hydrochloric acid in two carbohydrate solution. Result : Table 1 : The presence of starch and reducing sugar in the solution Observation Conclusion Solution A Benedict’s test : The transparent blue solution turns into opaque brick-red precipitate Iodine’s test : The clear colourless solution turns into transparent yellowish-brown
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The first half of module seven discussed protein digestion‚ absorption‚ and metabolism; while the second half focused on pathological stress and kidney disease. Protein digestion occurs in the stomach and small intestine. When a protein reaches the stomach it is denatured by hydrochloric acid. Once the protein is digested pepsin breaks apart the denatured protein into peptides. Peptides move into the small intestine where they encounter an enzyme that digest the peptides into di- or tripeptides‚
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Human Digestion Summary SCI 241 August 8‚ 2013 Human Digestion Summary We all love to eat. I remember my parents used to tell me that in order for my food to digest properly‚ I would have to chew it one hundred times on both side to make it small as possible. They said that if the food was smaller it could digest quicker and easier. But most people do not know what happens to their food after they have chewed it up and swallowed it. Some may think that right after the food is swallowed‚ it
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