DNA DIGESTION AND ELECTROPHORESIS In this experiment we will be doing a process called as DNA digestion or also known as restriction digest. A restriction digest is a procedure used in molecular biology to prepare DNA for analysis or other processing. It is sometimes termed DNA fragmentation‚ scientists Hartl and Jones describe it this way: This enzymatic technique can be used for cleaving DNA molecules at specific sites‚ ensuring that all DNA fragments that contain a particular sequence have the
Premium Molecular biology DNA
Title: Investigate the breakdown of starch at different temperature AIM This experiment has been done to investigate the action of the enzyme amylase on the breakdown of starch. MATERIALS These were the materials used: four starch/ agar plates‚ a marker pen‚ 1mm graph paper ruler‚ 8mm cork borer‚ forceps and template for cutting holes‚ 1% Amylase‚ water‚ incubator set at 5‚ 20‚ 40 and 60 degree Celsius. METHOD This was the experimental procedure carried out: the materials above were collected
Premium Enzyme
Digestion Lab Notebook Due Sunday at Midnight CST Week 6‚ Worth 100 points‚ pg. 105 Questions: 60 points Data/Results: 15 points Summarizing activities: 25 points Name: SC 245 Lab Date: Exercise 8 Chemical and Physical Processes of Digestion‚ pg. 105 Introduction: In this lab‚ I will learn how the complex digestive system breaks down substrates such as fats‚ proteins and carbohydrates. Activity 1: Assessing Starch Digestion by Salivary Amylase Data: Chart 1 – Salivary Amylase
Premium Enzyme Digestion Starch
IODIMETRIC AND IODOMETRIC METHOD SUBSTANCE TO BE ASSAY AQUEOUS/NON-AQUEOUS ALKALINITY / ACIDIMETRY DIRECT/RESIDUAL TITRATION TITRANT INDICATOR CHEMICAL REACTION Assay of Antimony potassium tartrate Direct titration 0.1 N Iodine Starch TS KOOCCHOHCHOHCOO (SbO) + I2 + H2O KOOCCHOHCHOHCOO (SbO2) + 2HI + 2HI + 2NaHCO3 2NaI + 2H2O +
Premium Chlorine Sodium Ammonia
Result Starch suspension When the yellowish-brown iodine was added into the starch suspension‚ the starch suspension was changed into dark blue. Potato slide Discussion Starch suspension When the yellowish-brown iodine was added into the starch suspension‚ the starch suspension was changed into dark blue. This is due to the yellowish-brown iodine changed into dark blue when there is a presence of starch. This shows a positive test for starch. Potato slide
Premium Protein Amino acid
This study focuses on the effect that temperature has on the enzyme amylase. Enzymes are composed of proteins and acts as a catalyst to speed up the rate of a specific chemical reaction by lowering the activation energy. Reactions do not need enzymes to occur‚ but the human body and other living organisms depend on the use of enzymes in order for biochemical reactions to happen in milliseconds. Each enzyme contains a distinct three dimensional structure which is related to its particular function
Premium Enzyme Catalysis Chemical reaction
fungus amylase and also discovers the optimum temperature for these enzymes. The amylase was mixed with starch at temperatures of 0℃‚ 37℃‚ 57℃‚ and 90℃. Iodine was added to each mixture and colour changes in each case. Bacteria amylase was found to be effective at 55 0C as the temperature dropped drastically from 4.58℃ to 2.33℃. This shows that the amylase catabolized a lot of starch hence little is left which cannot turn the iodine solution to blue-black which indicates the presence of starch. Fungal
Premium Enzyme Starch Amylase
Vol.3‚ No.6 (2009) 366–375 Available online at www.expresspolymlett.com DOI: 10.3144/expresspolymlett.2009.46 Starch-based completely biodegradable polymer materials D. R. Lu‚ C. M. Xiao*‚ S. J. Xu College of Material Science and Engineering of Huaqiao University‚ Quanzhou‚ 362021‚ P. R. China Received 21 February 2009; accepted in revised form 30 March 2009 Abstract. Starch is a natural polymer which possesses many unique properties and some shortcoming simultaneously. Some synthetic polymers
Premium Polymer Starch
In 1792 James painted a portrait called‚ “A Voluptuary in the Horrors of Digestion.” A voluptuary is someone who overindulges in pleasurable things. In this painting it shows‚ a Prince sitting on a fancy chair after eating a large meal. This Prince will someday be King. In order to be a good king they must have the following things‚ he must be ambitious‚ honest‚ and good with money. For the following reasons this Prince is not ready to be King. First‚ the king must be ambitious. Being ambitious
Premium English-language films Political philosophy Louis XIV of France
Correlation between the chemical activity of Amylase and change in temperature ABSTRACT This experiment focuses on how the change of temperature affects the rate of reaction of amylase. In the experiment there were four different environments that each contained 2 test tubes. Each test tube consisted of the same concentration and amount of starch and amylase. After having each test tube placed in these environments for several minutes a droplets of each mixture was placed onto each slot which
Premium Enzyme Chemical reaction Amylase