alpha-amylase enzyme with starch substrate and establish the optimum temperature and pH for this reaction. The optimum temperature and pH for the reaction of alpha-amylase and starch was predicted to be a temperature of 50°C and a pH of 5. The optimum temperature and pH for the reaction was determined by monitoring the reaction rate of alpha-amylase at different temperatures and pH’s by means of using a spectrophotometer to measure the disappearance (in absorbance) of the substrate starch. As a result
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the rate in which Amylase breaks down starch into maltose. In this reaction starch is the substrate and maltose is the product. Amylase is an enzyme‚ Enzymes‚ also called catalysts‚ are in living things and there are thousand of them. Enzymes break down food by the active site on the Enzyme forming a chemical bond with a substrate and then water attacks the substrate until it is hydrolysed (split in 2). Equipment: Boiling tubes Timers/ stopwatch Starch Solution of Amylase colourless Thermometer
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Defn. of Digestion: the process whereby a biological entity processes a substance‚ in order to chemically convert the substance into nutrients. It usually involves mechanical manipulation and chemical action. Mechanical Digestion What is it? This type of digestion involves the mixing‚ grinding or crushing of large pieces of food into smaller places. Why does it take place? So that the food we consume is broken down into smaller pieces before we swallow it which in turn makes process of
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Biology Form Four Digestion Digestion is the process by which food is broken down from complex insoluble substances into simple soluble substances. There are two types of digestion – chemical digestion and mechanical digestion. Mechanical digestion involves breaking up large pieces of food into smaller pieces. This provides a large surface area for the action of enzymes on food. There is no change to the chemical composition of food during mechanical digestion. Mechanical digestion occurs in the
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ruminants like cattle enables them to efficiently use high roughage feedstuffs‚ including forages. Their digestive system is composed of the mouth‚ tongue‚ gall bladder‚ pancreas‚ the four compartment stomach (rumen‚ reticulum‚ omasum‚ and abomasum)‚ salivary glands‚ the small intestine (duodenum‚ jejunum‚ and ileum)‚ esophagus and the large intestine (cecum‚ colon‚ and rectum) (Brooker‚ 5). Mouth and Teeth A ruminant animal uses its mouth and tongue to harvest forages and consume feedstuffs during
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There was a controlled and an experimental substance. The controlled substance was the one with starch in the dialysis bag‚ and the experimental substance was the one with starch and amylase in the dialysis bag. Both had the same solvent outside of the bag (Lugols and Distilled Water). The color change differed from inside and outside the bag as time went on‚ and at the end of the 45 minutes‚ the two bags had changed colors. The solute in the controlled substance had a darker color to it‚ with it
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PROCESS OF DIGESTION 1 Process of Digestion Amy Wallace EN 101 February 27‚ 2013 Miller-Motte Technical College PROCESS OF DIGESTION 2 Digestion is an important process needed for maintaining and providing energy to living organisms. Throughout the digestion process‚ food is broken down into small molecules‚ where it can be absorbed easily. There are many organs that aid
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Chemical and Physical Processes of Digestion NAME: LAB TIME/DATE: Carbohydrate Digestion The following questions refer to Activity 1: Assessing Starch Digestion by Salivary Amylase. 1. At what pH did you see the highest activity of salivary amylase? Why? 2. How do you know that the amylase did not have any contaminating maltose? 3. What effect did boiling have on enzyme activity? Why? 4. Describe the substrate and the subunit product of amylase. The following questions refer
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“Is Starch Permeable in Cells? Abstract This report presents the weights of 3 samples of water with various amounts of solute after the use of osmosis. Three different tests were performed‚ each with a beaker of water containing varying amounts of starch from 30% concentration (12.5 grams of water) to 15% concentration (9.5 grams of water)‚ and then 0% (10.5 grams of water). To start this experiment‚ we put water into a make-shift dialysis tube‚ a type of semi-permeable membrane tubing made
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Digestion/ Absorption and Homeostasis In order to survive‚ our bodies must bring in energy and nutrients for the cells of the body through eating and digestion of food. To be carried to the cells of the body by the blood stream‚ food must be broken down to molecules. This breaking down of food into molecules‚ small enough to be absorbed into and carried through the blood stream‚ is carried out by the digestive system through the process of digestion and absorption. Digestion is the process of
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