2. Steps for Southern Blotting Digest the DNA with an appropriate restriction enzyme. Run the digest on an agarose gel. Denature the DNA‚ which would separate double-stranded DNA into single-stranded DNA. Transfer the denatured DNA to the membrane. Probe the membrane. Visualize your radioactively labeled target sequence. If you used a radiolabeled 32P probe‚ then you would visualize by autoradiograph. 3. a) She placed the nucleotide as she did because the EcoR I cuts in one
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Abstract……………………………………………………………………………………………2 Introduction………………………………………………………………………………………..2 Background………………………………………………………………………………..2 Objectives…………………………………………………………………………………2 Scope………………………………………………………………………………………3 Theory review……………………………………………………………………………………..3 Design of report…………………………………………………………………………………...5 Procedures…………………………………………………………………………………………5 Results……………………………………………………………………………………………..6 Discussion…………………………………………………………………………………………6 Conclusion………………………………………………………………………………………...7 Reference………………………………………………………………………………………
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The purpose of this experiment was to acquaint the students with basic laboratory procedures‚ methods‚ and techniques; to introduce the use of basic laboratory measuring devices; to demonstrate different methods of manipulation of numerical quantities. DENSITY AND SPECIFIC GRAVITY Materials and Methods Part 1: Density of an Unknown Solid 1. We first were asked from our laboratory instructor to attain an unknown solid and were asked to note down the number of the solid. 2. Determine
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day’s production to be tested if in fact 95% of the Blu-ray players conform to specifications is 13.55%. ❷ The reliability of an electrical fuse is the probability that a fuse‚ chosen at random from production‚ will function under its designed conditions. A random sample of 1000 fuses
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LADDER DIAGRAM: FIGURE 53 LADDER PROGRAM FOR THE SHIFT AND CLOCK PROGRAM THE SIMULATION OPTION IS SELECTED: Figure 54 SIMULATING THE LADDER PROGRAM ERROR AND DEBUGGING: Once the simulation has started it checks for the errors in the ladder diagram‚ address etc. if there is an error‚ the output cannot be processed until the error is rectified. REMOVING THE DEFECTIVE PRODUCTS: Figure 55 REMOVING THE DETECTED BOTTEL 1 Figure 56 REMOVING THE DEFECTED BOTTLE 2 INPUT
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Question 2.1: (i) Calculate the number of electrons which will together weigh one gram. (ii) Calculate the mass and charge of one mole of electrons. * ------------------------------------------------- Answer (i) Mass of one electron = 9.10939 × 10–31 kg Number of electrons that weigh 9.10939 × 10–31 kg = 1 Number of electrons that will weigh 1 g = (1 × 10–3 kg) = 0.1098 × 10–3 + 31 = 0.1098 × 1028 = 1.098 × 1027 (ii) Mass of one electron = 9.10939 × 10–31 kg Mass of one mole of
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erical methPage 48 Licentiate Thesis / Nenad Glodic 6 NUMERICAL INVESTIGATIONS 6.1 Numerical Method Numerical simulations are carried out employing a commercial CFD code (ANSYS CFX v11). The solver is using a full-scale time-marching 3D viscous model. Underlying equations‚ three dimensional Navier-Stokes equations in their conservation form‚ are being solved by using a Finite Volume method‚ where equations are integrated over the finite control volumes. Thereby‚ the solution domain is subdivided
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For these equations to be equivalent there is one condition you must put on any real number a. The condition that must be put on a is that a cannot be 0. The main reason that a cannot be 0 is because when we substitute 0 for a in the second equation you must multiply x times 0. X times 0 is 0. Then when you put 0 in for a on
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Institute Physics Course 2014 - Rocket Lab Report Title: RockSim and Electronic Altimeter Measurement Techniques Name: Scarlet Henriquez Email: scarletpatricia94@aol.com LAB SESSION ___8____Assignment _____7_____ Due Date: ___7/8/14______ Objective: Launch a smaller rocket to learn how to compare RockSim predictions (computer based) to an actual instrument measurement carried by the rocket (Electronic Altimeter). This Lab shows how better the electronic measurement
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Case Analysis on Macpherson Refrigeration Limited Submitted by The role of the responder Linda Metzler the production planning manager is the main responder in this case‚ she has to come up with another optimal alternative that will have to be submitted to the plant’s General Manager. The plan has to be approved by the general manager for roll out starting next year. Key Issues In order to achieve excellent production capacity and reducing the overall costs the production
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