Abstract The main goal of the enzyme kinetics experiment was to see how the phosphatase-catalyzed hydrolysis of p-nitrophenyl produced p-nitrophenol in the presence of phosphate and fluoride ion inhibitors of various concentrations. The calculated Km constant was found to be 0.22 for all reactions. The Vmax values for each inhibition ion were 0.00986 for the phosphate ion and 0.00436 for the fluoride ion. The inhibitor constant‚ Ki‚ was determined to be 0.0967 for the phosphate ion. The inhibitor
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discuss how concentration ratios are used to determine total market shares within four specific industries. I will also discuss the levels of competition within those industries and how oligopolies can benefit society. Case‚ Fare‚ and Oster defines concentration ratio as the share of industry output in sales or employment accounted for by the top firms (2009). They are used to measure the total output produced by a certain number of firms within an industry. Four-firm concentration ratios
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____________________ Immobilizing an enzyme provides various analytical benefits‚ and can be done in a myriad of ways‚ with the most common being entrapment. For this study peroxidase (from horseradish)‚ an enzyme that catalyzes the cleavage of hydrogen peroxide into water‚ was entrapped within a polyacrylamide gel matrix. The gel matrix was formed by the addition of methylene bis-acrylamide (a cross linking agent) to acrylamide. The immobilized enzyme was then tested via spectrophotometric assay
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In this report‚ the concentration of total protein and albumin were calculated with the interpretation of a standard curve. To determine the concentration of total protein in human serum‚ a total protein assay was performed by using the Biuret reagent. Proteins react to this compound because it contains two amide bonds. When the Biuret reagents is introduced to a sample of protein‚ it forms a blue or violet complex. The concentration of albumin in human serum is determined by using the Bromocresol
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Runninghead: IP 1 Individual Project Unit 3 BUS305-0804A-07 Concentration Ratio Economists use concentration ratio to measure the degree of concentration in a market‚ computed as the percentage of the market output produced by the largest firms (O’Sullivan‚ Sheffrin‚ & Perez. 2008). One of predominantly concentration ratio used is the Four Firm Concentration Ratio. Four Firm Concentration Ratio isthe percentage of total output in a market produced by the four largest firms. In considering
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Biology Digestive Enzyme Experiment “Design an experiment in which you will investigate a digestive enzyme’s effect on digestion” Research Question: To determine the effect of enzyme concentration on the rate of digestion. This will be done by increasing the concentration of the enzyme diastase and investigate its effect on the rate of starch digestion. The rate will be determined by the amount of time it takes to completely digest the powdered starch‚ the complete digestion will be indicated by
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THE EFFECT OF CONCENTRATION ON REACTION RATE INTRODUCTION • Concentration is one factor which has an effect o the rate of a chemical reaction. In chemistry we describe concentration in terms of the number of moles of a substance there are in every cubic decimetre (litre) of solution‚ written as moles.dm-3 of M for short. • In this experiment you will find out how increasing the concentration of the acid solution changes the rate of the reaction between dilute hydrochloric acid and magnesium
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Purpose: To find how pH and temperature levels affect the enzyme Background: Enzymes are a type of protein vital to sustaining life‚ it works with vitamins and minerals as a biological catalyst‚ which lowers the activation energy for a reaction to occur. Each individual type of enzyme does a specific job‚ and they do not die‚ they are reused. For example‚ catalase is an enzyme found in almost all living cells that will break down hydrogen peroxide and turn it into water and oxygen. The
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environmental factors affect the enzyme activity rate. For the first experiment‚ where we tested the increase in concentration of enzyme with the substrate‚ we found that higher concentration of enzyme increases the rate of reaction of the enzyme. This is because more enzyme molecules are present‚ which allow more substrate molecules to get into the active sites of the enzyme (Sattler W& Esterbauer H). When calculating the absorbance of different enzyme concentration‚ it was noticeable that the absorbance
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Acid –Base Titration: A volume of 128 mL of 0.650 M Ba(OH)2 was required to completely neutralize 50.0 mL of nitric acid solution (HNO3). What is the concentration of the acid solution? Gravimetric Titration: Calculate the molar concentration of Ba2+ ions in a 500.0 mL sample of an unknown aqueous solution if 2.47 g BaSO4 is formed upon the addition of excess Na2SO4? Stoichiometry of Acid –Base Reactions: What volume of 0.800 M HCl is required to completely neutralize 35.0 g of Ca(OH)2
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