Potato Enzyme Lab INTRODUCTION An enzyme is a protein that speeds up or slows down a specific chemical reaction in an organism. A good rule of thumb is to remember that enzyme names end in “-ase”. This will help in identifying enzymes in further readings. Generally enzymes are catalysts. Hydrogen peroxide is a toxic chemical that is produced in many organisms during metabolism. Organisms must get rid of this toxin to survive. One reaction turns the hydrogen peroxide into water and oxygen. The
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Factors Affecting The rate of Enzyme Activity Prediction: As the temperature increases the rate of enzyme activity will also increase‚ thus increasing the rate of reaction. However‚ if the temperature is too high the enzyme will denature. Materials: 4 test tubes 2 small beakers A dozen filter paper disks Test tube rack Hydrogen peroxide (H2O2) Potato extract Forceps Thermometer Hot plate Large beaker Ice cubes Graduated cylinder Stopwatch Procedure: Step 1 Place 10 mL of potato
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Catalase Lab Introduction: Catalase is an enzyme normally found in many plant and animal tissues. Its purpose is to destroy toxic substances which may be introduced into cells. Also‚ some cells use catalase to destroy cellular debris or worn out organelles. In this lab‚ we will use a catalase solution from potatoes and determine the effect of temperature and pH on the action of this enzyme. The substrate of the enzyme will be 3% hydrogen peroxide (H2O2). Catalase works by the following mechanism:
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THE RATE OF ENZYME ACTIVITY. To investigate the effect that temperature has on enzyme activity I am going to use the enzyme amylase‚ which is used as a biological catalyst to break down starch‚ which cannot pass through the gut wall due to the size of the molecules‚ into smaller ones. Amylase is a carbohydrase‚ which converts starch to simple sugars in the Salivary Glands. Three features of all enzymes are: They are always proteins. They are specific in their actions and each enzyme controls one
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Experiment 2: Estimation of protein concentration Introduction Protein assays are designed to measure the total protein in a solution. Protein assays are quantitative if the protein to be assayed is available in sufficient quantity such that one is able to use it to create a standard curve. If this cannot be achieved‚ then a standard protein‚ such as albumin‚ may be used for a standard curve with the understanding that the results on the unknown protein are semi-quantitative. Since most proteins
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Acetylcholinesterase and Butyrylcholinesterase substrate selectivity and various acting cholinesterase inhibitors Introduction Cholinesterases are a group of enzymes present in mammals which breakdown certain neurotransmitters by hydrolyzing the ester bonds within a molecule (Rang & Dale‚ 2007). There are two major types of enzymes‚ acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). Though similar in structure‚ they differ in distribution‚ function and substrate specificity. AChE is found in red
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Hand-out 3: Market Concentration Specification Market concentration. Definition “’Market concentration’ is the degree to which the output of an industry is dominated by its largest producers.” In other words‚ how many of the sales in the market are accounted for by the biggest firms in that market. Firm Sales (£m) % Market Share A 56 B 43 C 22 D 12 E 3 F 1 Total 100% Calculate the 3 firm concentration ratio of this market. 3 firm concentration ratio = __________________________________________________________
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process to anabolic hormones of the human body. Intrinsically they have like outcomes on the human body to anabolic steroids‚ triggering fast weight and force advances‚ but of a minor scale cause of the frequency limiting consequence produced by the enzyme adaptation. However‚ this theoretical explanation is thought-out somewhat outdated due to the expansions which have been done in supplement science. Epistane is amongst the hottest upscale steroids obtainable today‚ and it is achieving attention very
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What is the Molar Concentration (Solute concentration) of a Potato? ------------------------------------------------- ------------------------------------------------- ------------------------------------------------- Design ------------------------------------------------- ------------------------------------------------- 1. What is the dependent variable for this experiment? ------------------------------------------------- The dependent variable is the concentration (solute) of the
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The Affects of pH on Peroxidase Enzyme Claire Bell Biology 1130 Jazmen Myers Section 36 Abstract Peroxidase is a turnip enzyme; it is used in the oxidation of hydrogen peroxide to lower activation energy‚ speeding up the reaction. The activity of peroxidase is highly dependent on its environment and most importantly the pH level. Peroxidase has been the focus of many recent studies and is believed to possibly reduce swelling among other things. We conducted an experiment testing
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