Enzyme Lab Introduction/ Abstract An enzyme is a substance produced by a living organism that acts as a catalyst to bring about a specific biochemical reaction. They are mainly made up of proteins and can tremendously speed up reactions. E. coli ( a bacterium) has about 1‚000 different types of enzymes floating around in its cytoplasm at any given time. Enzymes can be used to join and even break up molecules as shown in the diagram below. (1)
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The Effect of pH on the Rate of Enzyme Catalysis of Catalase Objectives: The objective of this lab was to develop a protocol to investigate the effect of an environmental variable on the catalytic function of an enzyme. More specifically‚ the objective was to perform an experiment in order to test the effect of pH on the function of the enzyme catalase. Introduction: Enzymes are proteins that act as catalysts for reactions. This simply means that enzymes lower the activation energy required
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The data from the experiment demonstrates that the catalase enzyme breaks down the hydrogen peroxide due to its harmful toxicity to the liver. In section A‚ the effect surface area has on the enzyme was tested. The results have proven that as the surface area increases‚ the reaction rate of the enzyme also increases. To illustrate‚ when the liver was ground‚ the bubbles from the reaction reached a maximum height of 150mm in five seconds less than the unground liver which merely reached a maximum
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Experiment 2: Estimation of protein concentration Introduction Protein assays are designed to measure the total protein in a solution. Protein assays are quantitative if the protein to be assayed is available in sufficient quantity such that one is able to use it to create a standard curve. If this cannot be achieved‚ then a standard protein‚ such as albumin‚ may be used for a standard curve with the understanding that the results on the unknown protein are semi-quantitative. Since most proteins
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Hand-out 3: Market Concentration Specification Market concentration. Definition “’Market concentration’ is the degree to which the output of an industry is dominated by its largest producers.” In other words‚ how many of the sales in the market are accounted for by the biggest firms in that market. Firm Sales (£m) % Market Share A 56 B 43 C 22 D 12 E 3 F 1 Total 100% Calculate the 3 firm concentration ratio of this market. 3 firm concentration ratio = __________________________________________________________
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What is the Molar Concentration (Solute concentration) of a Potato? ------------------------------------------------- ------------------------------------------------- ------------------------------------------------- Design ------------------------------------------------- ------------------------------------------------- 1. What is the dependent variable for this experiment? ------------------------------------------------- The dependent variable is the concentration (solute) of the
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Concentration of a Solution: Beer’s Law The objective of this experiment is to determine the concentration of an unknown copper sulfate solution. You will be using the Colorimeter. In this device‚ red light from the LED light source will pass through the solution and strike a photocell. A higher concentration of the coloured solution absorbs more light (and transmits less) than a solution of lower concentration. The Colorimeter monitors the light received by the photocell as either an absorbance
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BIOLOGY IA A Factor That Will Affect Enzyme Activity The Question Effects on Enzyme activity: investigating the effects of mixing Hydrogen Peroxide with Catalase extracted from potatoes at different temperatures‚ and then comparing the results with the same method‚ except to replace the Potato juice with MnO2 Hypothesis Since enzymes are a type of protein‚ they will be destroyed at high temperatures and activity rates will go down at low temperatures. Also‚ if done at low temperature
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CONCENTRATION AND DILUTION Physiology 1‚ Las Positas College Name: In science‚ concentration is a measure of the number of particles (solutes) in a given volume. If one room has 100 people in it‚ and a room of equal size has 50 people‚ one can say that the concentration of people in one room is twice that of the other. Quite simple‚ isn’t it? On a molecular level‚ consider whether you put one lump or two of sugar‚ or no sugar at all‚ in your coffee. If you use two lumps‚ you prefer twice
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Osmosis Lab: Potatoes and Elodea Background Information: Cells have a need to regulate their internal environment. They need to be able to this because cells often find themselves in environments where the concentration of dissolved solids outside the cell is different from the number of dissolved solids inside the cell. Since the solids can not move across the membrane‚ the cell responds by moving water either into or out of the cell in an attempt to balance the number dissolved particles.
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